Eotaxin / CCL11 antibody

Principal name

Eotaxin / CCL11 antibody

Alternative names for Eotaxin / CCL11 antibody

C-C motif chemokine 11, Small-inducible cytokine A11, Eosinophil chemotactic protein, SCYA11

SwissProt ID

P48298 (Mouse), P51671 (Human), P97545 (Rat), Q9TTS6 (Bovin)

Gene ID

6356 (CCL11), 20292 (Ccl11)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Rat, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Neutralisation (NEUT), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of Eotaxin / CCL11 antibody

Eotaxin, a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines, was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Eotaxin has been found in three species (human, mouse, and guinea pig), which share approximately 60 % sequence homology. Mouse eotaxin also shows high sequence identity to the MCP family of cytokines. Mouse eotaxin cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature mouse eotaxin. Mature mouse eotaxin has a predicted molecular mass of approximately 8.4 kDa. Eotaxin is a potent eosinophil activator and chemoattractor. As an activator, eotaxin is a mediator of inflammation by initiating actin polymerization intracellularly and inducing the production of reactive oxygen species. As a chemoattractant both in vivo and in vitro, eotaxin exhibits potent activity on eosinophils, but not mononuclear cells or neutrophils. Eotaxin has the ability to prime eosinophils for chemotaxis, direct their migration, and to activate inflammatory activity. Eotaxin mRNA is expressed in a variety of tissues. Its expression is induced in cultured endothelial cells in response to IFN-gamma. Eotaxin mRNA is also induced in response to the transplantation of IL-4- secreting tumor cells. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3). The mouse counterpart to human CCR3 has also been isolated. This receptor for mouse eotaxin binds both mouse and human eotaxin with equal affinity.

General readings

Kakinuma T,., et al. (2002) Cytokine. 20(1):1-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Eotaxin / CCL11 (C-term) antibody

Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

To detect hEotaxin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEotaxin. Goat IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

To detect hEotaxin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Eotaxin (TA328300) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin. Goat IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue.  The recommended concentration is 0.50-0.75 ug/ml with an overnight incubation at 4C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.  Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

To detect mEotaxin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEotaxin. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

To detect mEotaxin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Eotaxin (TA328510) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Eotaxin / CCL11 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCL11 (RC204726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCL11. Mouse IgG1 OTI2H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Mouse IgG1 A132D 48F9 Purified Hu E(capture)
0.5 mg / €540.00
  Acris Antibodies GmbH

Eotaxin / CCL11 (C-term) antibody

Western blot analysis using Eotaxin antibody on HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Eotaxin / CCL11 Control Peptide

Eotaxin / CCL11
50 µg / €160.00
  Acris Antibodies GmbH

Eotaxin / CCL11 antibody

Western Blot analysis of CCL11 expression in transfected 293T cell line (H00006356-T01) by CCL11 MaxPab polyclonal antibody.

Lane 1: CCL11 transfected lysate(10.67 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Western Blot analysis of CCL11 expression in transfected 293T cell line (H00006356-T01) by CCL11 MaxPab polyclonal antibody.

Lane 1: CCL11 transfected lysate(10.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Mouse IgG1 1C8-2C1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Mouse IgG1 S1 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Rabbit Ms WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Western blot analysis using Eotaxin polyclonal antibody on HeLa cell lysate containing 2.5 ng of full length recombinant eotaxin. Rabbit IgG Hu WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 antibody

Immunocytochemistry of CCL11 in 293 cells with CCL11 polyclonal antibody ( Cat # PAB12824 ) at 5 µg/ml. Rabbit Hu ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Eotaxin / CCL11 (C-term) antibody

Mouse Spleen; WB Suggested Anti-Ccl11 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Spleen; Ccl11 antibody - C-terminal region (ARP30865_P050) in Mouse Spleen cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

Eotaxin / CCL11 antibody

Eotaxin / CCL11 Rabbit IgG Aff - Purified Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

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