EP300 / P300 antibody

Principal name

EP300 / P300 antibody

Alternative names for EP300 / P300 antibody

p300 HAT, KAT3B, Histone acetyltransferase p300, E1A-associated protein p300, E1A binding protein p300

SwissProt ID

Q09472 (Human)

Gene ID

2033 (EP300)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Md (Mustelid), Mink, Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), ChIP assay (ChIP), Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot)

Background of EP300 / P300 antibody

Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

General readings

General / background references:Dallas PB et al. p300/CREB binding protein-related protein p270 is a component of mammalian SWI/SNF complexes. Mol Cell Biol 18:3596-603 (1998). PubMed PMID: 9584200
Dallas PB et al. Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP. Hybridoma 16:273-5 (1997). PubMed PMID: 9219037
Dallas PB et al. Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes. J Virol 71:1726-31 (1997). PubMed PMID: 8995708

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

CBP/P300 antibody

CBP/P300 Mouse NM11 Aff - Purified Hu, Mky, Ms, Rt C, ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

EP300 / P300 (731-831) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1D2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

EP300 / P300 antibody

Immunoperoxidase of monoclonal antibody to EP300 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG1 1D2 Purified Hu E, ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

EP300 / P300 (C-term) antibody

Human breast carcinoma stained with anti-p300 antibody (Cat# AP15704PU). Rabbit Purified Hu, Ms, Rt P
0.5 ml / €410.00
  Acris Antibodies GmbH

EP300 / P300 (C-term) antibody

Human breast carcinoma stained with anti-p300 antibody (Cat# AP15704PU). Rabbit Purified Hu, Ms, Rt P
1 ml / €580.00
  Acris Antibodies GmbH

EP300 / P300 (C-term) antibody

Human breast carcinoma stained with anti-p300 antibody (Cat# AP15704PU). Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

EP300 / P300 antibody

Detection of p300 in a HeLa nuclear extract using anti-p300 (1:250).  ECL: 15 second exposure. Mouse IgG1 RW128 Purified Hu, Ms, Prim, Rt ICC/IF, IP, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis of extracts from MDA-MB-435 cells, using p300 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis of extracts from COLO205 cells, using P300/CBP Antibody (#TA313820).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis of extracts from 293 cells, using p300 (Ab-89) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis on 293 cell lysate using p300 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis of p300 expression in HepG2 cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis of P300/CBP expression in COLO205 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EP300 / P300 antibody

Western blot analysis using EP300 mouse mAb against truncated EP300-His recombinant protein (1). Mouse IgG1 7D8A6 Ascites Hu WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EP300 / P300 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1B1 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

EP300 / P300 antibody

Western blot analysis of p300 antibody (Cat.-No.: AP06548PU-N) in extracts from Hela cells. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EP300 / P300 pSer89 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EP300 / P300 pSer1834 antibody

Dot blot analysis of Phospho-P300-Ser1834 Antibody (Cat#AP12753PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

EP300 / P300 antibody

Immunohistochemistry analysis with  EP300 / P300 antibody (Cat.-No AP20224PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EP300 / P300 antibody

EP300 / P300 Rabbit Supernatant Hu E, EMSA, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

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