EPHA2 / ECK antibody
Principal name
EPHA2 / ECK antibody
Alternative names for EPHA2 / ECK antibody
Ephrin type-A receptor 2, Epithelial cell kinase, Eph receptor A2, Tyrosine-protein kinase receptor ECK
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Flow Cytometry (F), Frozen Sections (C), Immunoprecipitation (IP)
Background of EPHA2 / ECK antibody
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.
Detection limit for recombinant GST tagged EPHA2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T01) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
EPHA2 MaxPab polyclonal antibody. Western Blot analysis of EPHA2 expression in SW-13.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody ( Cat # PAB3003 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The EPHA2 polyclonal antibody ( Cat # PAB3003 ) is used in Western blot to detect EPHA2 in CHO cell lysate ( Lane 1 ) and NIH/3T3 cell lysate ( Lane 2 ) .
Immunofluorescence of monoclonal antibody to EPHA2 on A-431 cell. [antibody concentration 10 ug/ml]
EPHA2 monoclonal antibody (M02), clone 1E3. Western Blot analysis of EPHA2 expression in Jurkat ( Cat # L017V1 ).
EPHA2 monoclonal antibody (M02), clone 1E3 Western Blot analysis of EPHA2 expression in A-431 ( Cat # L015V1 ).
EPHA2 monoclonal antibody (M02), clone 1E3. Western Blot analysis of EPHA2 expression in U-2 OS ( Cat # L022V1 ).
EPHA2 MaxPab polyclonal antibody. Western Blot analysis of EPHA2 expression in human pancreas.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T02) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Thyroid stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Testis stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry: Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 antibody (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Western blot analysis on mouse brain: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
LEFT: Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. MIDDLE: Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Flow Cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (AP14274PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Western blot analysis of EphA2 (arrow) using rabbit polyclonal EphA2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA2 gene (Lane 2)
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) using EPHA2 antibody
Paraffin embedded sections of human colon ( A ) and gastric cancer tissue ( B ) were incubated with EPHA2 monoclonal antibody, clone 3D7 ( Cat # MAB1769 ) ( 1:100 ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine ( DAB ).
The extracts of mouse brain ( 50 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with EPHA2 monoclonal antibody, clone 3D7 ( Cat # MAB1769 ) ( 1:500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T02) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(108.30 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Eph receptor A2 Antibody (8F21) [NB110-93602] - EphA2 immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.
Immunohistochemistry-Paraffin: Eph receptor A2 Antibody [NBP1-18913] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-EphA2 used at a dilution of 1:250. DAB staining using Immunohistochemistry Accessory Kit
Western Blot and Immunoprecipitation: EphA2 Antibody [NBP1-18913] -Whole cell lysate from H2009 cells. EphA2 was successfully immunoprecipitated by rabbit anti-EphA2 antibody NBP1-18914.
ELISA: Eph receptor A2 Antibody [26620002]
Figure.2: Spectral Confocal Microscopy of CHO cells using EphA2 antibody (Cat.#DM1226). CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of EphA2 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Figure.3: SDS-PAGE analysis of purified EphA2 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 µg of purified EphA2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Figure.1: FACS analysis of BOSC23 cells using EphA2 antibody (Cat.#DM1226). BOSC23 cells were transiently trans-fected with an expression vector encoding either EphA2 (Red curve) or an irrelevant protein (control transfectant). Binding of EphA2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with EphA2 transfected cells.
Western blot (WB) analysis of EphA2/3/4 antibody (Cat.-No.: AP20225PU-N) in extracts from Hela cells.
Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Skin, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis using anti-Human EphA2 monoclonal antiobdy against NIH3T3 cell lysate.
Immunohistochemistry-Paraffin: Eph receptor A2 Antibody (1B3C7) [NBP1-47400] - Immunohistochemical analysis of paraffin-embedded human skin carcinoma (left) and pancreas carcinoma (right) tissue, showing cytoplasmic localization using EphA2 mouse mAb with DAB staining.
Western Blot: Eph receptor A2 Antibody (1B3C7) [NBP1-47400] - Western blot analysis using EphA2 mouse mAb against NIH3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded human skin carcinoma (left) and pancreas carcinoma (right) tissue, showing cytoplasmic localization using EphA2 antibody Cat.-No AM06122SU-N with DAB staining.
Western blot analysis using EphA2 antibody Cat.-No AM06122SU-N against NIH/3T3 cell lysate.
Human breast carcinoma stained with anti-EPHA2 / ECK Antibody Cat.-No AM32431PU (Clone SP169).
Flow Cytometric analysis of Rabbit anti-EPHA2 / ECK Antibody Cat.-No AM32431PU (Clone SP169) in A431 (green) compare to negative control of Rabbit IgG (blue).
Western blot analysis: Antibody Cat.-No. AP32671PU-N (1/1000) can detect EPH A2/A3/A4/A5 (pTyr596/602) in A431 cell lysates; Lane 1: no stimulation with EGF at 0.1ug/ml, lane 2: stimulation with EGF (+) at 0.1ug/ml; Arrow corresponds to MW of approx. 140 kDa.
Western blot analysis using anti-human EphA2 monoclonal antiobdy against NIH3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded human skin carcinoma(left) and pancreas carcinoma(right) tissue, showing cytoplasmic localization using anti-EphA2 antibody with DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated(bs-0485R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.
Western blot analysis of extracts from JK cells (Lane 2), using EPHA2/5 (Phospho-Tyr594) Antibody AP55847PU-N. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of EPHA2 expression in A431 (A); H1299 (B) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHA2 (RC205725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHA2.
ELISA: Eph receptor A2 Antibody
Western blot analysis of EPHA2 (pY588/596) expression in A431 (A); mouse brain (B) whole cell lysates.
Immunofluorescent analysis of EPHA2 (pY588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of EPHA2 expression in HeLa (A); Jurkat (B); NIH3T3 (C) whole cell lysates.
Immunofluorescent analysis of EPHA2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| CPA4632-30ul | EPHA2 pTyr588/pTyr596 antibody |
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 |
Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
30 µl /
€210.00
|
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| Cohesion Biosciences Ltd. | |||||||
 +1 additional image |
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| CPA4632-100ul | EPHA2 pTyr588/pTyr596 antibody |
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|
Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.1 ml /
€330.00
|
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| Cohesion Biosciences Ltd. | |||||||
| +1 additional image |
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| CPA4632-200ul | EPHA2 pTyr588/pTyr596 antibody |
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Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.2 ml /
€530.00
|
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| Cohesion Biosciences Ltd. | |||||||
| +1 additional image |
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| CPA4633-30ul | EPHA2 antibody |
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 |
Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
30 µl /
€190.00
|
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| Cohesion Biosciences Ltd. | |||||||
| +1 additional image |
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| CPA4633-100ul | EPHA2 antibody |
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|
Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.1 ml /
€270.00
|
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| Cohesion Biosciences Ltd. | |||||||
| +1 additional image |
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| CPA4633-200ul | EPHA2 antibody |
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|
Rabbit | Aff - Purified | Chk, Hu, Mky, Ms, Rt | ICC/IF, WB |
0.2 ml /
€440.00
|
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| Cohesion Biosciences Ltd. | |||||||
| +1 additional image |
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| AP55721PU-N | EPHA2/3/4 pTyr588/596 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€340.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP55721PU-S | EPHA2/3/4 pTyr588/596 antibody |
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Rabbit | Aff - Purified | Hu, Ms | ICC/IF, WB |
50 µl /
€270.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AM09008PU-N | EPHA2 / ECK antibody |
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Mouse | IgG2b | 3D7 | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€310.00
|
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM09008PU-S | EPHA2 / ECK antibody |
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|
Mouse | IgG2b | 3D7 | Purified | Hu, Ms | E, P, WB |
50 µl /
€230.00
|
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| H00001969-M02 | EPHA2 / ECK antibody |
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![Immunofluorescence of monoclonal antibody to EPHA2 on A-431 cell. [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00001969-M02-4-4-1-L.jpg) |
Mouse | IgG3 | 1E3 | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM32431PU-M | EPHA2 / ECK antibody |
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Rabbit | IgG | SP169 | Purified | Hu | F, P |
0.5 ml /
€520.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| AM32431PU-N | EPHA2 / ECK antibody |
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Rabbit | IgG | SP169 | Purified | Hu | F, P |
1 ml /
€730.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| AM32431PU-S | EPHA2 / ECK antibody |
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|
Rabbit | IgG | SP169 | Purified | Hu | F, P |
0.1 ml /
€360.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
Acris recommends this product for Immunohistochemistry. |
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| TA313798 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA313799 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313801 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA347325 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu, Rt | WB |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA347931 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590631 | EPHA2 / ECK antibody |
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 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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