EPHA2 / ECK antibody

Principal name

EPHA2 / ECK antibody

Alternative names for EPHA2 / ECK antibody

Ephrin type-A receptor 2, Epithelial cell kinase, Eph receptor A2, Tyrosine-protein kinase receptor ECK

SwissProt ID

P29317 (Human), Q03145 (Mouse)

Gene ID

1969 (EPHA2), 13836 (Epha2)

Available reactivities

Hu (Human), Hst (Hamster), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), Mky (Monkey), Rt (Rat), Chk (Chicken)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Flow Cytometry (F)

Background of EPHA2 / ECK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHA2/3/4 pTyr588/596 antibody

Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

EPHA2/3/4 pTyr588/596 antibody

Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €270.00
  Acris Antibodies GmbH

EPHA2 / ECK antibody

Western Blot analysis: The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: EphA2 extracellular domain transfected 293T cell lysate. Lane 3: EphA2 cytoplasmic domain transfected 293T cell lysate. Mouse IgG2b 3D7 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

EPHA2 / ECK antibody

Western Blot analysis: The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: EphA2 extracellular domain transfected 293T cell lysate. Lane 3: EphA2 cytoplasmic domain transfected 293T cell lysate. Mouse IgG2b 3D7 Purified Hu, Ms E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EPHA2 / ECK antibody

  Rabbit polyclonal EPHA2/3/4 (Ab-588/596) antibody  
Western blot analysis of extracts from NIH/3T3 cells, using EPHA2/3/4 (Ab-588/596) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit polyclonal anti-EPHA2 antibody  
Western blot analysis of extracts from COLO205 and Jurkat cells, using EPHA2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit polyclonal EPHA2/5 (Ab-594) antibody  
Western blot analysis of extracts from JK cells, using EPHA2/5 (Ab-594) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit Polyclonal Anti-EPHA2/3/4 Antibody  
Western blot analysis on HepG2 cell lysate using EPHA2/3/4 Antibody Rabbit IgG Purified Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit Polyclonal Anti-EPHA2 Antibody  
Western blot analysis of EPHA2 expression in Jurkat cells Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit Polyclonal Anti-EPHA2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (EPHA2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit Monoclonal anti-EphA2 Antibody  
EPHA2 / ECK Rabbit IgG SP169 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

  Rabbit Polyclonal EPH Receptor A2 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHA2 (RC205725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHA2. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHA2 / ECK antibody

Immunohistochemical analysis of paraffin-embedded human skin carcinoma (left) and pancreas carcinoma (right) tissue, showing cytoplasmic localization using EphA2 antibody Cat.-No AM06122SU-N with DAB staining. Mouse IgM 1B3C7 Ascites Hu P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EPHA2 / ECK antibody

Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgM 1B3C7 Ascites Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EPHA2 / ECK (450-500) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) using EPHA2 antibody Rabbit IgG Aff - Purified Bov, Can, Eq, Hu, Mky, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

EPHA2 / ECK (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hst, Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHA2 / ECK (C-term) antibody

Western blot analysis of EphA2 (arrow) using rabbit polyclonal EphA2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA2 gene (Lane 2) Rabbit Ig Purified Hst, Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHA2 / ECK (+EPHA3/4) antibody

Western blot (WB) analysis of EphA2/3/4 antibody (Cat.-No.: AP20225PU-N) in extracts from Hela cells. Rabbit Aff - Purified Hu, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EPHA2 / ECK antibody

Figure.2: Spectral Confocal Microscopy of CHO cells using EphA2 antibody (Cat.#DM1226). 
CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of EphA2 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue). Mouse IgG1 GM5H5 Purified Hu E, E(detection), F, ICC/IF
0.1 mg / €360.00
  Acris Antibodies GmbH

EPHA2 / ECK pTyr588/596 antibody

Immunofluorescence analysis of HeLa cells, using EPHA2/3 (Phospho-Tyr588/596) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

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