EPHA2 / ECK antibody
Principal name
EPHA2 / ECK antibody
Alternative names for EPHA2 / ECK antibody
Ephrin type-A receptor 2, Epithelial cell kinase, Eph receptor A2, Tyrosine-protein kinase receptor ECK
Ncbi ID
9606, NP_004422, NP_004422.2, NM_004431.2, NM_010139, NM_004431
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Flow Cytometry (F)
Background of EPHA2 / ECK antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Detection limit for recombinant GST tagged EPHA2 is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T01) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
EPHA2 MaxPab polyclonal antibody. Western Blot analysis of EPHA2 expression in SW-13.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA2 polyclonal antibody ( Cat # PAB3003 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
The EPHA2 polyclonal antibody ( Cat # PAB3003 ) is used in Western blot to detect EPHA2 in CHO cell lysate ( Lane 1 ) and NIH/3T3 cell lysate ( Lane 2 ) .
Immunofluorescence of monoclonal antibody to EPHA2 on A-431 cell. [antibody concentration 10 ug/ml]
EPHA2 monoclonal antibody (M02), clone 1E3. Western Blot analysis of EPHA2 expression in Jurkat ( Cat # L017V1 ).
EPHA2 monoclonal antibody (M02), clone 1E3 Western Blot analysis of EPHA2 expression in A-431 ( Cat # L015V1 ).
EPHA2 monoclonal antibody (M02), clone 1E3. Western Blot analysis of EPHA2 expression in U-2 OS ( Cat # L022V1 ).
EPHA2 MaxPab polyclonal antibody. Western Blot analysis of EPHA2 expression in human pancreas.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T02) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis: The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane 2: EphA2 extracellular domain transfected 293T cell lysate. Lane 3: EphA2 cytoplasmic domain transfected 293T cell lysate.
Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Thyroid stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Testis stained with EPHA2 antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry: Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 antibody (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
LEFT: Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. MIDDLE: Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Flow Cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (AP14274PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
Western blot analysis of EphA2 (arrow) using rabbit polyclonal EphA2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA2 gene (Lane 2)
Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) using EPHA2 antibody
Paraffin embedded sections of human colon ( A ) and gastric cancer tissue ( B ) were incubated with EPHA2 monoclonal antibody, clone 3D7 ( Cat # MAB1769 ) ( 1:100 ) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine ( DAB ).
The extracts of mouse brain ( 50 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with EPHA2 monoclonal antibody, clone 3D7 ( Cat # MAB1769 ) ( 1:500 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot analysis of EPHA2 expression in transfected 293T cell line (H00001969-T02) by EPHA2 MaxPab polyclonal antibody.Lane 1: EPHA2 transfected lysate(108.30 KDa).Lane 2: Non-transfected lysate.
Figure.2: Spectral Confocal Microscopy of CHO cells using EphA2 antibody (Cat.#DM1226). CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of EphA2 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Figure.3: SDS-PAGE analysis of purified EphA2 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 µg of purified EphA2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Figure.1: FACS analysis of BOSC23 cells using EphA2 antibody (Cat.#DM1226). BOSC23 cells were transiently trans-fected with an expression vector encoding either EphA2 (Red curve) or an irrelevant protein (control transfectant). Binding of EphA2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with EphA2 transfected cells.
Western blot (WB) analysis of EphA2/3/4 antibody (Cat.-No.: AP20225PU-N) in extracts from Hela cells.
Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Skin, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis using anti-Human EphA2 monoclonal antiobdy against NIH3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded human skin carcinoma (left) and pancreas carcinoma (right) tissue, showing cytoplasmic localization using EphA2 antibody Cat.-No AM06122SU-N with DAB staining.
Western blot analysis using EphA2 antibody Cat.-No AM06122SU-N against NIH/3T3 cell lysate.
Human breast carcinoma stained with anti-EPHA2 / ECK Antibody Cat.-No AM32431PU (Clone SP169).
Flow Cytometric analysis of Rabbit anti-EPHA2 / ECK Antibody Cat.-No AM32431PU (Clone SP169) in A431 (green) compare to negative control of Rabbit IgG (blue).
Western blot analysis of extracts from HepG2 cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunofluorescence staining of methanol-fixed HeLa cells using EPHA2/3/4 (Phospho-Tyr588/596) Antibody AP55721PU-N.
Western blot analysis of extracts from JK cells (Lane 2), using EPHA2/5 (Phospho-Tyr594) Antibody AP55847PU-N. The lane on the left is treated with antigen-specific peptide.
Western blot analysis of EPHA2 expression in A431 (A); H1299 (B) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHA2 (RC205725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHA2.
ELISA: Eph receptor A2 Antibody
Western blot analysis of EPHA2 (pY588/596) expression in A431 (A); mouse brain (B) whole cell lysates.
Immunofluorescent analysis of EPHA2 (pY588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of EPHA2 expression in HeLa (A); Jurkat (B); NIH3T3 (C) whole cell lysates.
Immunofluorescent analysis of EPHA2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ��C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of extracts from NIH/3T3 cells, using EPHA2/3/4 (Ab-588/596) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using EPHA2/3/4 (Ab-588/596) Antibody. The lane on the left is treated with synthesized peptide.
Immunofluorescence analysis of A549 cells, using EPHA2/3/4 (Ab-588/596) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from COLO205 and Jurkat cells, using EPHA2 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells, using EPHA2/5 (Ab-594) Antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2), using EPHA2/5 (Ab-594) Antibody. The lane on the left is treated with synthesized peptide.
Western blot analysis on HepG2 cell lysate using EPHA2/3/4 Antibody
Western blot analysis of EPHA2 expression in Jurkat cells
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (EPHA2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
