EPHB1 antibody

Principal name

EPHB1 antibody

Alternative names for EPHB1 antibody

EPHT2, NET, HEK6, ELK, EPH2, Ephrin type-B receptor 1, Tyrosine-protein kinase receptor EPH-2

SwissProt ID

P09759 (Rat), P54762 (Human), Q07494 (Chick), Q8CBF3 (Mouse), Q91571 (Xenla)

Gene ID

2047 (EPHB1), 270190 (Ephb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken)

Available hosts

Sheep, Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of EPHB1 antibody

The membrane bound Ephrins are ligands for Ephrin receptor tyrosine kinases that regulate several physiological functions including long-term potentiation, cell positioning, CNS development and axonal path finding and mitogenesis etc. Ephrins molecules exhibit the ability to send bidirectional signals following ligand-receptor interactions resulting from cell-cell contacts. Activation of Eph receptors are also shown to regulate transduction pathways important in cell cycle control as well as cell death. There are at least 4 different kinds of Ephrin B receptors are cloned (Ephrin B1R-Ephrin B4R). EphB receptor tyrosine kinases and ephrin-B ligands also regulate several types of cell-cell interactions during brain development, generally by modulating the cytoskeleton. EphB/ephrinB genes are expressed in the developing neural tube of early mouse embryos with distinct overlapping expression in the ventral midbrain. The positioning of cells within the stem cell niche in the intestinal epithelium is controlled by B subclass ephrins through their interaction with EphB receptors. Ephrin 3 ligands are also known as guidance molecule as they are mainly responsible for positioning and directing the cell migration along the villus-crypt axis in intestine. Ephrin 3 by interacting with ephrin B3 receptors in conjunction with Wnt proteins signals and promotes cell cycle re-entry of proginator cells and accounts for 50% of the mitogenic activity. The Ephrin B receptors are the key coordinators of the intestinal stem cell niche (1).
Ephrin B3 ligands are highly expressed in the mossy fibers axons and terminals and the Ephrins B3 receptors are expressed on post-synaptic neurons. Ephrin-B3 promotes adhesion of hippocampus neurons to the ligand-expressing substrates in vitro, and the loss of the receptor or EphB2 abrogates the effects of ephrin-B3 (2). The function of Ephrins in the immune system has not been well studied, although some Ephrins and Ephrin receptors are expressed at high levels on certain leukocytes. EFNB3 and its cognate receptors are expressed in peripheral T cells and monocytes/macrophages. The role of EphR2 and EphR4 in angiogenesis and formation of arteries and veins come form findings that EphR2 marks arteries but not veins and conversely, the EphR4 labels exclusively the veins (3).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHB1 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHB1/2/3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB1 antibody

Western blot analysis of extracts from 3T3 cells, treated with heat shock, using EPHB1/2/3/4 (Ab-600/602/614/596) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB1 antibody

Western blot analysis of extracts from HepG2 cells, using EPHB1/2 (Ab-594/604) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB1 antibody

Western blot analysis of extracts from COS7 cells, using EPHB1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB1 antibody

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 1-2. Anti-EphB1 (extracellular) antibody, (1:800). 3-4. Anti-EphB1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

EPHB1 antibody

Western blot analysis of EPHB1 expression in COS7 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB1 antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and colon cancer (right) showing cytoplasmic localization with DAB staining using EphB1 antibody Cat.-No AM06214SU-N Mouse IgG1 5F10A4 Ascites Hu E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

EPHB1 (EXT Region) antibody

EPHB1 Sheep IgG Purified Hu IP
0.25 mg / €360.00
  Acris Antibodies GmbH

EPHB1 (SAM Region) antibody

EPHB1 Sheep IgG Purified Hu IP
0.25 mg / €360.00
  Acris Antibodies GmbH

EPHB1 (Cytopl. Dom.) antibody

EPHB1 Sheep IgG Purified Hu IP
0.25 mg / €360.00
  Acris Antibodies GmbH

EPHB1 Control Peptide

EPHB1
0.25 mg / €250.00
  Acris Antibodies GmbH

EPHB1 antibody

EPHB1 Rabbit Aff - Purified Hu E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

EPHB1 (C-term) antibody

(TOP)Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells. (BUTTOM)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB1 antibody

Western blot analysis of EphB1 (arrow) using rabbit polyclonal EphB1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 gene (Lane 2) Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB1 (+EphB2) antibody

Immunohistochemistry analyzes of EphB1/2 antibody (Cat.-No.: AP20300PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EPHB1 (+EphB2/3/4) antibody

Western blot (WB) analysis of EphB1/2/3/4 antibody (Cat.-No.: AP21112PU-N) in extracts from 3T3 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EPHB1 antibody

Western Blot analysis of EPHB1 expression in transfected 293T cell line (H00002047-T01) by EPHB1 MaxPab polyclonal antibody.

Lane 1: EPHB1 transfected lysate(108.24 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

EPHB1 antibody

Western Blot analysis of EPHB1 expression in transfected 293T cell line (H00002047-T01) by EPHB1 MaxPab polyclonal antibody.

Lane 1: EPHB1 transfected lysate(108.24 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

EPHB1 antibody

EPHB1 Mouse IgG2a 4G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EPHB1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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