EPHB2 antibody

Principal name

EPHB2 antibody

Alternative names for EPHB2 antibody

Ephrin type-B receptor 2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, NY-REN-47, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, Tyrosine-protein kinase TYRO5, Renal carcinoma antigen NY-REN-47

SwissProt ID

P28693 (Chick), P29323 (Human), P54763 (Mouse)

Gene ID

2048 (EPHB2)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Hst (Hamster), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Selected product citations ‐ of AM11064PU-N

Sara Poirier Culleton. Submitted. REGULATION OF PAPILLOMAVIRUS E2 PROTEIN BY POST-TRANSLATIONAL MODIFICATION, Thesis 2015.
https://scholarworks.iupui.edu/bitstream/handle/1805/7346/Culleton_iupui_0104D_10043.pdf?sequence=1&isAllowed=y

Background of EPHB2 antibody

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

General readings

Cramer KS, Cerretti DP, Siddiqui SA.
EphB2 regulates axonal growth at the midline in the developing auditory brainstem. Dev Biol. 2006 Apr 13
PMID: 16626680

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHB2 (17-200) antibody

Tonsil, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 2D12C6 Ascites Hu E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EPHB2 antibody

  Goat Polyclonal Antibody against EPHB2  
TA303150 (1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

  Rabbit polyclonal anti-EPHB2 antibody  
Western blot analysis of extracts from Jurkat cells, using EPHB2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

  Rabbit Polyclonal Anti-EPHB2 Antibody (C-Terminus)  
Anti-EPHB2 / EPH Receptor B2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €400.00
  OriGene Technologies, Inc.

EPHB2 antibody

  Rabbit Polyclonal Anti-EPHB2 Antibody (Internal)  
Anti-EPHB2 / EPH Receptor B2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Chk, Hst, Hu, Por
50 µg / €400.00
  OriGene Technologies, Inc.

EPHB2 antibody

  Rabbit Polyclonal Anti-EPHB2 Antibody  
Western blot analysis of EPHB2 expression in Jurkat cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 antibody Cat.-No AM06225SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG2b 2D12C6 Ascites Hu E, ICC/IF, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

EPHB2 antibody

Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 Monoclonal Antibody (Cat.#AM11064PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse IgG1 48CT12.6.4 Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

EPHB2 (C-term) antibody

(TOP)Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EphB2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 antibody

Western blot analysis of extracts from Jurkat cells, using EPHB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

EPHB2 antibody

Western blot analysis of extracts from Jurkat cells, using EPHB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

EPHB2 - human gene knockout kit via CRISPR

EPHB2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EPHB2 - human gene knockout kit via CRISPR

EPHB2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPHB2 - human gene knockout kit via CRISPR

EPHB2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EPHB2 - human gene knockout kit via CRISPR

EPHB2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EPHB2 (C-term DDK/His tag, transcript variant 1)

  Purified recombinant protein of human EPH receptor B2 (EPHB2), transcript variant 1, with C-terminal DDK/His tag, expressed in human cells, 20 µg  
EPHB2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EPHB2 (mGFP-tagged)-Human EPH receptor B2 (EPHB2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, EPHB2 (mGFP-tagged)-Human EPH receptor B2 (EPHB2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,020.00
  OriGene Technologies, Inc.

Lenti ORF particles, EPHB2 (mGFP-tagged)-Human EPH receptor B2 (EPHB2), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, EPHB2 (mGFP-tagged)-Human EPH receptor B2 (EPHB2), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €2,020.00
  OriGene Technologies, Inc.

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