EPHB2 antibody

Principal name

EPHB2 antibody

Alternative names for EPHB2 antibody

Ephrin type-B receptor 2, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, NY-REN-47, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, Tyrosine-protein kinase TYRO5, Renal carcinoma antigen NY-REN-47

SwissProt ID

P28693 (Chick), P29323 (Human), P54763 (Mouse)

Gene ID

2048 (EPHB2), 396513

Available reactivities

Hu (Human), Ms (Mouse), Ze (Zebrafish), Rt (Rat), Can (Canine), Chk (Chicken), Hst (Hamster), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Selected product citations ‐ of AM11064PU-N

Sara Poirier Culleton. Submitted. REGULATION OF PAPILLOMAVIRUS E2 PROTEIN BY POST-TRANSLATIONAL MODIFICATION, Thesis 2015.
https://scholarworks.iupui.edu/bitstream/handle/1805/7346/Culleton_iupui_0104D_10043.pdf?sequence=1&isAllowed=y

Background of EPHB2 antibody

EphB2, also known as Cek5, Hek5, Tyro5, Se3, Nuk, Erk, Qek2, and Drt, is a member of the Eph receptor family, which binds members of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. The recombinant mouse EphB2 used for the testing of this antibody consists of the extracellular domain of mouse EphB2 (amino acids 1-548)1 fused by means of a polypeptide linker to the Fc portion of human IgG1 that is histidine-tagged at the C-terminus. N-terminal sequencing indicates that recombinant mouse EphB2 has Val 27 at the amino terminus. The calculated molecular mass of the reduced protein is approximately 85.3 kDa, but as a result of glycosylation, recombinantEphB2/Fc migrates as a 100-110 kDa protein under reducing conditions in SDS-PAGE. EphB2 binds to ephrin-B1, ephrin-B2, and ephrin-B3. Human and mouse EphB3 extracellular domains share approximately 99% homology. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.

General readings

Cramer KS, Cerretti DP, Siddiqui SA. EphB2 regulates axonal growth at the midline in the developing auditory brainstem. Dev Biol. 2006 Apr 13; [Epub ahead of print] PMID: 16626680

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHB2 (17-200) antibody

Tonsil, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2b 2D12C6 Ascites Hu E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EPHB2 antibody

TA303150 (1µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

Western blot analysis of extracts from Jurkat cells, using EPHB2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

Anti-EPHB2 / EPH Receptor B2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

EPHB2 antibody

Anti-EPHB2 / EPH Receptor B2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Chk, Hst, Hu, Por
50 µg / €379.00
  OriGene Technologies, Inc.

EPHB2 antibody

Western blot analysis of EPHB2 expression in Jurkat cells Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB2 antibody

Immunofluorescence analysis of Hela (left) and HepG2 (right) cells using EphB2 antibody Cat.-No AM06225SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG2b 2D12C6 Ascites Hu E, ICC/IF, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

EPHB2 antibody

Figure 2. Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 Monoclonal Antibody (Cat.#AM11064PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse IgG1 48CT12.6.4 Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

EPHB2 (C-term) antibody

(TOP)Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EphB2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (BUTTOM)Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EphB2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EphB2 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB2 antibody

EPHB2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

EPHB2 antibody

EPHB2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

EPHB2 antibody

EPHB2 Mouse IgM 1F10 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

EPHB2 antibody

EPHB2 Mouse IgG2a 3B3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EPHB2 antibody

EPHB2 monoclonal antibody (M03), clone 4D1 Western Blot analysis of EPHB2 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 4D1 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EPHB2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB2 polyclonal antibody ( Cat # PAB3019 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

EPHB2 antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EPHB2 polyclonal antibody ( Cat # PAB3020 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

EPHB2 antibody

EPHB2 polyclonal antibody ( Cat # PAB7121 ) ( 1 µg/ml ) staining of human lung lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

EPHB2 antibody

Western blot analysis of extracts from Jurkat cells, using EPHB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

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