EPHB4 antibody

Principal name

EPHB4 antibody

Alternative names for EPHB4 antibody

Ephrin type-B receptor 4, HTK, TYRO11, Tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11

SwissProt ID

P54760 (Human), P54761 (Mouse)

Gene ID

2050 (EPHB4), 13846

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Goat, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Flow Cytometry (F)

Background of EPHB4 antibody

The monoclonal antibody VEB4-7E4 recognizes mouse Ephrin type-B receptor 4 (EphB4), an ~110 kD protein. Erythropoietin-producing human hepatocellular carcinoma (Eph) receptors and ephrins are membrane proteins. They are classified into 2 broad subclasses, namely A and B, according to structural homologies and binding specificities. Eph receptors are tyrosine kinases, which autophosphorylate upon binding to their cognate ephrin ligands. Eph receptors and ephrins are frequently expressed in reciprocal patterns that correlate with cellular boundaries during embryonic development. The interaction between EphB4 and its ligand, Ephrin-B2, plays an important role in cell-cell, cell-extracellular matrix interactions as well as in cell migration, adhesion and proliferation. During the early stages of vascular development, EphB4 is specifically expressed in venous endothelium, whereas Ephrin-B2 is expressed in arterial endothelium. In mouse embryo?s, EphB4 and Ephrin-B2 are essential for embryonic heart development and angiogenesis. In adult microcirculation, EphB4 is not a ubiquitous marker of arterial/venous polarity, but is expressed along both venules and arterioles. Furthermore, EphB4 is upregulated by endothelial cells along blind-ended capillary sprouts versus connecting capillaries. As such, EphB4 is thought to play a role in the patterning of new vessels during angiogenesis. EphB4 is also expressed in a variety of tumor cells, like gastrointestinal, prostate, bladder, breast, liver, lung and ovarian cancers, as well as leukemia, mesothelioma, malignant breast tumors and melanoma. Reduction of EphB4 activity accelerated tumorigenesis in colon and rectum. In head and neck squamous cell carcinoma and endometrial carcinoma, overexpression of EphB4 is inversely related to a poor prognosis. However, in mesothelioma, up-regulation of EphB4 resulted in growth of the tumor. Besides the essential expression of EphB4, coexpression of other EphB4 family members or EphB-ligands may affect tumor cell viability and proliferation as well.

General readings

1. Doleman JF  et al. Identification of the Eph receptor pathway as a novel target for eicosapentaenoic acid (EPA) modification of gene expression in human colon adenocarcinoma cells (HT-29). Nutr Metab (Lond) 7:56 (2010). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EPHB4 (Extracell. Dom.) antibody

Colon, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 5B5 Ascites Hu E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EPHB4 antibody

Immunohistochemistry analysis of EphB4 Antibody (Cat.-No AP20388PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EPHB4 antibody

Western blot analysis of extracts from 293 cells and Jurkat cells, using EPHB4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EPHB4 antibody

Western blot analysis of EPHB4 expression in 293 cell extract Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EPHB4 antibody

Immunohistochemical analysis of paraffin-embedded Human pancreas carcinoma (left) and breast carcinoma (right) tissue, showing membrane and cytoplasmic (pancreas carcinoma) localization, membrane (breast carcinoma) localization using EphB4 antibody Cat.-No AM06124SU-N with DAB staining. Mouse IgG2a 5B8F7 Ascites Hu E, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EPHB4 antibody

Western blot analysis using EphB4 mouse mAb against Jurkat (1) and HEK293 (2) cell lysate. Mouse IgG1 7H4A6 Ascites Hu WB
0.1 ml / €360.00
  Acris Antibodies GmbH

EPHB4 antibody

Confocal Immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 antibody Cat.-No AM06328SU-N (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 5B5 Ascites Hu ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EPHB4 antibody

Figure 1.Western blot analysis of anti-EPHB4 Monoclonal Antibody (Cat.#AM11066PU-N) by EPHB4 recombinant protein(Fragment). EPHB4 protein (arrow) was detected using the purified Mab. Mouse IgG1 67CT23.5.8 Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB4 antibody

EPHB4 Rat IgG2a VEB4-7E4 Purified Ms C, E, ICC/IF, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

EPHB4 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with the EphB4 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EPHB4 antibody

Detection limit for recombinant GST tagged EPHB4 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1D1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

EPHB4 antibody

Detection limit for recombinant GST tagged EPHB4 is approximately 3ng/ml as a capture antibody. Mouse IgG2b 4G11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EPHB4 antibody

EPHB4 Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

EPHB4 antibody

Western blot analysis of extracts from Jurkat/293 cells, using EPHB4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

EPHB4 antibody

Western blot analysis of extracts from Jurkat/293 cells, using EPHB4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

EPHB4 antibody

Western blot analysis of EPHB4 expression in A549 (A); Jurkat (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EPHB4 antibody

Western blot analysis of EPHB4 expression in A549 (A); Jurkat (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EPHB4 antibody

Western blot analysis of EPHB4 expression in A549 (A); Jurkat (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EPHB4 antibody

EPHB4 Rabbit Hu E, P, WB
50 µg / €400.00
  Novus Biologicals Inc.

EPHB4 antibody

Immunohistochemistry: EPH receptor B4 Antibody (6A14) [NB110-93607] - EphB4 immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals. Rat IgG1 6A14 Purified Ms WB
0.1 mg / €450.00
  Novus Biologicals Inc.

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