Ephrin-B1 antibody

Principal name

Ephrin-B1 antibody

Alternative names for Ephrin-B1 antibody

EFL-3, EFNB1, EFL3, EPLG2, LERK2, ELK-L, LERK-2, CEK5-L, EPH-related receptor tyrosine kinase ligand 2, CEK5 LIgand

SwissProt ID

O13097 (Xenla), O73612 (Chick), P52795 (Mouse), P52796 (Rat), P98172 (Human)

Gene ID

394292 (efnb1), 395896 (EFNB1), 1947, 13641 (Efnb1)

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Frog, Can (Canine), Xen (Xenopus), Ze (Zebrafish), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Rat

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of Ephrin-B1 antibody

Ephrin-B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). Ephrin-B molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin-B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin-B is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific Ephrin-B residue (Tyr298) plays a key role in Ephrin-B signaling (Kalo, et al., 2001).

General readings

Nagashima K., et al. (2002) Mol Biol Cell. 13:4231-4242.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Ephrin-B1 pTyr317 antibody

Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr317 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr317 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Ephrin-B1 antibody

Western blot analysis of EFNB1/2 expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Ephrin-B1 antibody

WB Suggested Anti-EFNB1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human kidney Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Ephrin-B1 (+Ephrin-B2) antibody

Western blot analysis of Ephrin-B antibody (Cat.-No. AP06387PU-N) in extracts from 293 cells treated with EGF 200ng/ml 5' or 293 cells treated with TNF-a 20ng/ml 30'. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Ephrin-B1 pTyr298 antibody

Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Ephrin-B1 pTyr331 antibody

Figure 1. Western blot of rat testes lysate showing specific immunolabeling of the ~46k Ephrin-B phosphorylated at Tyr331 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr331 Ephrin-B. The immunolabeling of the Ephrin-B band is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, Rt, Xen, Ze WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Ephrin-B1 antibody

Western blot analysis of Ephrin-B1 antibody (AP20226PU-N) in extracts from COS7 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Ephrin-B1 pTyr317 antibody

Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr317 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y317) polyclonal antibody ( Cat # PAB9611 ). The immunolabeling of the EphrinB is completely eliminated by treatment with l-Ptase. Rabbit IgG Bov, Chk, Hu, Ms, Rt
0.1 ml / €510.00
  Abnova Taiwan Corp.

Ephrin-B1 pTyr331 antibody

Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr331 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y331) polyclonal antibody ( Cat # PAB9612 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit IgG Bov, Chk, Hu, Ms, Rt
0.1 ml / €510.00
  Abnova Taiwan Corp.

Ephrin-B1 pTyr298 antibody

Western blot of rat testis lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the EphrinB (phospho Y298) polyclonal antibody ( Cat # PAB9690 ). The immunolabeling of the EphrinB band is completely eliminated by treatment with l-Ptase. Rabbit IgG Bov, Chk, Frog, Hu, Ms, Rt
0.1 ml / €510.00
  Abnova Taiwan Corp.

Ephrin-B1 pTyr317 antibody

Western blot analysis of extracts from mouse brain, using EFNB1 (Phospho-Tyr317) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Ephrin-B1 pTyr317 antibody

Western blot analysis of extracts from mouse brain, using EFNB1 (Phospho-Tyr317) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Ephrin-B1 pTyr329 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2 (Phospho-Tyr329) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

Ephrin-B1 pTyr329 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2 (Phospho-Tyr329) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

Ephrin-B1 pTyr324 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Ephrin-B1 pTyr324 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Ephrin-B1 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using EFNB1/2 (Ab-330) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Ephrin-B1 antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using EFNB1/2 (Ab-330) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Ephrin-B1 antibody

Western blot analysis of extracts from COS7 cells, using EFNB1 (Ab-317) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Ephrin-B1 antibody

Western blot analysis of extracts from COS7 cells, using EFNB1 (Ab-317) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

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