Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Can (Canine)
Western Blot analysis of EPHX1 expression in transfected 293T cell line (H00002052-T02) by EPHX1 MaxPab polyclonal antibody.Lane 1: EPHX1 transfected lysate(50.05 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EPHX1 expression in transfected 293T cell line (H00002052-T01) by EPHX1 MaxPab polyclonal antibody.Lane 1: EPHX1 transfected lysate(52.90 KDa).Lane 2: Non-transfected lysate.
The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: The extracts of mouse liver (30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The extracts of mouse liver ( 30 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with EPHX1 monoclonal antibody, clone AT2E5 ( Cat # MAB3588 ; 1 : 1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Human Liver; WB Suggested Anti-EPHX1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; EPHX1 antibody - middle region (ARP54286_P050) in Human Liver cells using Western Blot
Human Jurkat; WB Suggested Anti-EPHX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; EPHX1 antibody - N-terminal region (ARP54285_P050) in Human Jurkat cells using Western Blot
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800395)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
HEK293T cells transfected with either RC200621 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EPHX1 antibody(TA800408), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800409)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800409)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800439)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800439)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(TA308516) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(TA308517) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/10-25 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)