Epoxide hydrolase 1 / EPHX1 antibody
Principal name
Epoxide hydrolase 1 / EPHX1 antibody
Alternative names for Epoxide hydrolase 1 / EPHX1 antibody
EPHX, EPOX, Epoxide hydratase, Microsomal epoxide hydrolase
SwissProt ID
P04068 (Rabit), P07099 (Human), P07687 (Rat), P79381 (Pig), Q9D379 (Mouse), Q9NQV0 (Human)
Ncbi ID
9606, NP_000111, 9986, NM_000120, NP_000111.1, NP_001129490, NM_001136018
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Luminex (LMNX), Paraffin Sections (P), Flow Cytometry (F)
Background of Epoxide hydrolase 1 / EPHX1 antibody
- Kit Component:
- - KN200621G1, EPHX1 gRNA vector 1 in pCas-Guide vector
- KN200621G2, EPHX1 gRNA vector 2 in pCas-Guide vector
- KN200621D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Bertvan Loo, et al., (2006) Appl Environ Microbiol 72(4):2905-2917.
Janet K.C, et al., (2001) The Histochemical Journal 33:329-336.
Detection limit for recombinant GST tagged EPHX1 is approximately 3ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to EPHX1 on HepG2 cell. [antibody concentration 10 ug/ml]
EPHX1 MaxPab polyclonal antibody. Western Blot analysis of EPHX1 expression in human liver.
Western Blot analysis of EPHX1 expression in transfected 293T cell line (H00002052-T02) by EPHX1 MaxPab polyclonal antibody.Lane 1: EPHX1 transfected lysate(50.05 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of EPHX1 expression in transfected 293T cell line (H00002052-T01) by EPHX1 MaxPab polyclonal antibody.Lane 1: EPHX1 transfected lysate(52.90 KDa).Lane 2: Non-transfected lysate.
The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis: The extracts of mouse liver (30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The extracts of mouse liver ( 30 µg ) were resolved by SDS-PAGE, transferred to NC membrane and probed with EPHX1 monoclonal antibody, clone AT2E5 ( Cat # MAB3588 ; 1 : 1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Human Liver; WB Suggested Anti-EPHX1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; EPHX1 antibody - middle region (ARP54286_P050) in Human Liver cells using Western Blot
Human Jurkat; WB Suggested Anti-EPHX1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; EPHX1 antibody - N-terminal region (ARP54285_P050) in Human Jurkat cells using Western Blot
EPHX1 ELISA with 2C1 Capture (TA600435) and 4G4 Detection (TA700435) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 2C1 Capture (TA600435) and 4G4 Detection (TA700435) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 2D8 Capture (TA600436) and 3F10 Detection (TA700436) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 2D8 Capture (TA600436) and 3F10 Detection (TA700436) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3A10 Capture (TA600437) and 2C1 Detection (TA700437) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3A10 Capture (TA600437) and 2C1 Detection (TA700437) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3F10 Capture (TA600438) and 3F11 Detection (TA700438) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3F10 Capture (TA600438) and 3F11 Detection (TA700438) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3F11 Capture (TA600439) and 2D8 Detection (TA700439) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3F11 Capture (TA600439) and 2D8 Detection (TA700439) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 2C1 Capture (TA600435) and 4G4 Detection (TA700435) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 2C1 Capture (TA600435) and 4G4 Detection (TA700435) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 2D8 Capture (TA600436) and 3F10 Detection (TA700436) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 2D8 Capture (TA600436) and 3F10 Detection (TA700436) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3A10 Capture (TA600437) and 2C1 Detection (TA700437) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3A10 Capture (TA600437) and 2C1 Detection (TA700437) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3F10 Capture (TA600438) and 3F11 Detection (TA700438) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3F10 Capture (TA600438) and 3F11 Detection (TA700438) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 ELISA with 3F11 Capture (TA600439) and 2D8 Detection (TA700439) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
EPHX1 Luminex ELISA with 3F11 Capture (TA600439) and 2D8 Detection (TA700439) Antibodies. Substrate used: Recombinant Human EPHX1 (TP300621)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800395)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800407)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
HEK293T cells transfected with either RC200621 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EPHX1 antibody(TA800408), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800409)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800409)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800418)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800439)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800439)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (RC200621, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . 10% SDS PAGE. TA308516 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1(TA308516) antibody at 1:500 dilution.
EPHX1 antibody detects EPHX1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. EPHX1 antibody (TA308516) dilution: 1:500.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(TA308516) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate). A: H1299. B: Hela. 10% SDS PAGE. TA308517 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded SG xenograft, using Epoxide hydrolase 1(TA308517) antibody at 1:100 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using EPHX1(TA308517) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/10-25 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification:x 200)
Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/25-50 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using EPHX1 antibody at dilution 1/20, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of extracts of various cell lines, using EPHX1 antibody.
Immunohistochemical of paraffin-embedded human esophageal cancer using EPHX1 antibody at dilution of 1:100 (400x lens).
Immunohistochemical of paraffin-embedded human esophageal cancer using EPHX1 antibody at dilution of 1:100 (200x lens).
- Quick-Filters:
- Highly Validated (5)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308516 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Rabbit Polyclonal antibody to Epoxide hydrolase 1 (epoxide hydrolase 1, microsomal (xenobiotic)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA800407 | Epoxide hydrolase 1 / EPHX1 antibody |
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| EPHX1 (Epoxide Hydrolase 1) mouse monoclonal antibody, clone OTI3F10 (formerly 3F10) | |||||||
 |
Mouse | IgG2b | OTI3F10 | Purified | Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA800409 | Epoxide hydrolase 1 / EPHX1 antibody |
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| EPHX1 (Epoxide Hydrolase 1) mouse monoclonal antibody, clone OTI4G4 (formerly 4G4) | |||||||
 |
Mouse | IgG2a | OTI4G4 | Purified | Can, Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA800418 | Epoxide hydrolase 1 / EPHX1 antibody |
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| EPHX1 (Epoxide Hydrolase 1) mouse monoclonal antibody, clone OTI3F11 (formerly 3F11) | |||||||
 |
Mouse | IgG2b | OTI3F11 | Purified | Can, Hu, Mky, Ms | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA800439 | Epoxide hydrolase 1 / EPHX1 antibody |
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| EPHX1 (Epoxide Hydrolase 1) mouse monoclonal antibody, clone OTI2D8 (formerly 2D8) | |||||||
 |
Mouse | IgG2b | OTI2D8 | Purified | Can, Hu, Mky | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA308517 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Rabbit Polyclonal antibody to Epoxide hydrolase 1 (epoxide hydrolase 1, microsomal (xenobiotic)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA321060 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Anti-EPHX1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321061 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Anti-EPHX1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA327254 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Rabbit anti-EPHX1 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA600435 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Luminex and ELISA validated EPHX1 mouse monoclonal antibody (clone 2C1, clone OTI2C1 (formerly 2C1) | |||||||
 |
Mouse | IgG1 | OTI2C1 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA600436 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex and ELISA validated EPHX1 mouse monoclonal antibody (clone 2D8, clone OTI2D8 (formerly 2D8) | |||||||
 |
Mouse | IgG1 | OTI2D8 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA600437 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex and ELISA validated EPHX1 mouse monoclonal antibody (clone 3A1, clone OTI3A10 (formerly 3A10) | |||||||
 |
Mouse | IgG1 | OTI3A10 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA600438 | Epoxide hydrolase 1 / EPHX1 antibody |
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| Luminex and ELISA validated EPHX1 mouse monoclonal antibody (clone 3F1, clone OTI3F10 (formerly 3F10) | |||||||
 |
Mouse | IgG1 | OTI3F10 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA600439 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex and ELISA validated EPHX1 mouse monoclonal antibody (clone 3F1, clone OTI3F11 (formerly 3F11) | |||||||
 |
Mouse | IgG1 | OTI3F11 | Purified | Hu | E, LMNX |
0.1 mg /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700435 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex validated biotinylated EPHX1 mouse monoclonal antibody (clone 4G4), clone OTI4G4 (formerly 4G4) | |||||||
 |
Mouse | IgG1 | OTI4G4 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700436 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex validated biotinylated EPHX1 mouse monoclonal antibody (clone 3F10), clone OTI3F10 (formerly 3F10) | |||||||
 |
Mouse | IgG1 | OTI3F10 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700437 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex validated biotinylated EPHX1 mouse monoclonal antibody (clone 2C1), clone OTI2C1 (formerly 2C1) | |||||||
 |
Mouse | IgG1 | OTI2C1 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700438 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex validated biotinylated EPHX1 mouse monoclonal antibody (clone 3F11), clone OTI3F11 (formerly 3F11) | |||||||
 |
Mouse | IgG1 | OTI3F11 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA700439 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| Luminex validated biotinylated EPHX1 mouse monoclonal antibody (clone 2D8), clone OTI2D8 (formerly 2D8) | |||||||
 |
Mouse | IgG1 | OTI2D8 | Purified | Hu | E, LMNX |
50 µg /
€345.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA800395 | Epoxide hydrolase 1 / EPHX1 antibody |
||||||
| EPHX1 (Epoxide Hydrolase 1) mouse monoclonal antibody, clone OTI3A10 (formerly 3A10) | |||||||
 |
Mouse | IgG1 | OTI3A10 | Purified | Hu, Mky | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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