ERAS / HRAS2 antibody

Principal name

ERAS / HRAS2 antibody

Alternative names for ERAS / HRAS2 antibody

GTPase ERas, E-Ras, HRASP, Embryonic stem cell-expressed Ras

SwissProt ID

Q7TN89 (Mouse), Q7Z444 (Human)

Gene ID

3266 (ERAS), 353283 (Eras)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ERAS / HRAS2 antibody

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERAS / HRAS2 antibody

  Rabbit polyclonal anti-ERAS antibody  
Western blot analysis of extracts from Jurkat cells, using ERAS antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 (N-term) antibody

  Rabbit polyclonal ERAS Antibody (N-term) (F66)  
Western blot analysis of ERAS Antibody (N-term) (F66) (Cat. #TA324562) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

  Rabbit Polyclonal Anti-Eras Antibody  
WB Suggested Anti-Eras Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

  Rabbit Polyclonal Anti-ERAS Antibody  
WB Suggested Anti-ERAS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

  Rabbit Polyclonal Anti-ERAS Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse heart tissue, Primary antibody: (ERAS Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

  ERAS mouse monoclonal antibody, clone OTI2C6 (formerly 2C6)  
Anti-ERAS mouse monoclonal antibody (TA503689) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAS(RC210965). Mouse IgG2b OTI2C6 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ERAS / HRAS2 antibody

  ERAS mouse monoclonal antibody, clone OTI3A3 (formerly 3A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAS (RC210965, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAS. Mouse IgG1 OTI3A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERAS / HRAS2 antibody

Western blot (WB) analysis of E-Ras antibody (Cat.-No.: AP01406PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ERAS / HRAS2 (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (AP11463PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ERAS / HRAS2 antibody

Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

ERAS / HRAS2 antibody

Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ERAS - human gene knockout kit via CRISPR

ERAS - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ERAS - human gene knockout kit via CRISPR

ERAS - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ERAS - human gene knockout kit via CRISPR

ERAS - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eras - mouse gene knockout kit via CRISPR

Eras - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Eras - mouse gene knockout kit via CRISPR

Eras - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eras - mouse gene knockout kit via CRISPR

Eras - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eras - mouse gene knockout kit via CRISPR

Eras - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ERAS / HRAS2

  Recombinant protein of human ES cell expressed Ras (ERAS)  
ERAS / HRAS2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ERAS overexpression lysate

ERAS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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