ERAS / HRAS2 antibody

Principal name

ERAS / HRAS2 antibody

Alternative names for ERAS / HRAS2 antibody

GTPase ERas, E-Ras, HRASP, Embryonic stem cell-expressed Ras

SwissProt ID

Q7TN89 (Mouse), Q7Z444 (Human)

Gene ID

3266 (ERAS), 353283 (Eras)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ERAS / HRAS2 antibody

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

Items 1 to 20 of 28 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERAS / HRAS2 antibody

Western blot analysis of extracts from Jurkat cells, using ERAS antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 (N-term) antibody

Western blot analysis of ERAS Antibody (N-term) (F66) (Cat. #TA324562) in HL60 cell line lysates (35ug/lane). ERAS (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

WB Suggested Anti-Eras Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

WB Suggested Anti-ERAS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ERAS / HRAS2 antibody

Anti-ERAS mouse monoclonal antibody (TA503689) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERAS(RC210965). Mouse IgG2b OTI2C6 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ERAS / HRAS2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERAS (RC210965, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERAS. Mouse IgG1 OTI3A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERAS / HRAS2 antibody

Western blot (WB) analysis of E-Ras antibody (Cat.-No.: AP01406PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ERAS / HRAS2 (N-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (N-term) (AP11463PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ERAS / HRAS2 antibody

Western Blot analysis of ERAS expression in transfected 293T cell line (H00003266-T01) by ERAS MaxPab polyclonal antibody.

Lane 1: ERAS transfected lysate(25.63 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ERAS / HRAS2 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody ( Cat # PAB3129 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

ERAS / HRAS2 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS polyclonal antibody ( Cat # PAB3130 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

ERAS / HRAS2 antibody

Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

ERAS / HRAS2 antibody

Immunofluorescence analysis of A549 cells, using ERAS Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

ERAS / HRAS2 (N-term) antibody

Mouse Heart; WB Suggested Anti-Eras Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Eras antibody - N-terminal region (ARP55793_P050) in Mouse Heart cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ERAS / HRAS2 antibody

Human Lung; WB Suggested Anti-ERAS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; ERAS antibody - middle region (ARP55794_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ERAS / HRAS2 antibody

Immunofluorescent analysis of ERAS staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ERAS / HRAS2 antibody

Immunofluorescent analysis of ERAS staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ERAS / HRAS2 antibody

Immunofluorescent analysis of ERAS staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ERAS / HRAS2 antibody

Western Blot: E-Ras Antibody [NBP1-00813] - Western blot (WB) analysis of E-Ras (C226) antibody in extracts from Jurkat cells Rabbit Aff - Purified Hu ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ERAS / HRAS2 antibody

Western Blot: ERAS Antibody [NBP1-58322] - This Anti-ERAS antibody was used in Western Blot of Fetal Lung   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Items 1 to 20 of 28 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn