ERBB4 / HER4 antibody

Principal name

ERBB4 / HER4 antibody

Alternative names for ERBB4 / HER4 antibody

p180erbB4, c-ErbB-4, HER-4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4

SwissProt ID

Q15303 (Human), Q61527 (Mouse), Q62956 (Rat)

Gene ID

2066 (ERBB4), 59 (ACTA2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bt (Bat), Eq (Equine), Mky (Monkey), Bov (Bovine), Can (Canine), Hst (Hamster)

Available hosts

Mouse, Rabbit

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C)

Background of ERBB4 / HER4 antibody

The tyrosine kinase receptor erbB4, also known in humans as Her4, is a member of the epidermal growth factor receptor (EGFR or erbB1) family, which also includes erbB2 and erbB3 that are gene products of proto-oncogenes. Protocogenes play an important role in normal proliferation of cells. The interaction of proto-oncogenes with other tumor suppressor proteins (P53, P14ARF etc.) determine the normal or abnormal course of cell cycle and cell division leading to uncontrolled cell proliferation (1). Her 2 is found on chromosome 17 and codes for a protein that serves as a cell surface receptor. Her protein family of receptor has 4 structurally similar members of membrane receptors that have intrinsic tyrosine kinase activity. These Her receptors working in concert, determine the regulation of cell growth and differentiation in a complex manner. Her-2 receptor is still considered to be an orphan receptor with no identifiable ligand. Her family receptors can form homo or hetero dimers with other compounds. The activation of these homo-heterodimmeric complexes by ligand binding led to an activation of intrinsic tyrosine kinase activity that triggers a cascade of signal transduction activity in cell. Some of these signals are transduced across the cell membranes in to cytoplasm and triggers the gene activation. A better understanding of these receptors and identification of their respective ligands will greatly enhance our understanding of cell cycle and uncontrolled cell division as seen in cancerous tissues. Several steps in these pathways are currently being targeted for therapeutic intervention, including tyrosine kinase inhibitors. There are at least 2 humanized recombinant monoclonal antibodies (Trastuzumab and pertuzumab) that target extra cellular regions of the Her 2 tyrosine kinase receptor and have been used in human trials for the treatment of breast cancer (2).
Herceptin-4 is a 1308 amino acid protein (approximate MW 170-172 kDa) when analyzed on reduced SDS-PAGE gels. Her4 is over expressed in cells of many cancer types. It’s over expression is nicely correlated with a poor prognosis of breast and ovarian cancer in patients. ErbB receptors bind to a family of growth factors, termed neuregulins/heregulin (NRG/HRG), which comprise NRG-1, -2, -3, and -4 and include multiple variants. ErbB4/Her4 is an orphan receptor that does not bind ligand alone but heterodimerizes with the other erbB receptors for NRG signaling. Her 4 is a receptor tyrosine kinase with intrinsic tyrosine kinase domain.

General readings

General / background references:Tang CK et al. Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo. Cancer Res 59:5315-22 (1999). PubMed PMID: 10537315
Chen X et al. An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4. J Biol Chem 271:7620-9 (1996). PubMed PMID: 8631797

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERBB4 / HER4 (C-term) antibody

Human breast carcinoma stained with anti-c-erbB-4 antibody Rabbit Purified Hu P, WB
1 ml / €490.00
  Acris Antibodies GmbH

ERBB4 / HER4 (C-term) antibody

Human breast carcinoma stained with anti-c-erbB-4 antibody Rabbit Purified Hu P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

ERBB4 / HER4 antibody

ERBB4 / HER4 Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERBB4 / HER4 antibody

Anti-ERBB4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu
50 µg / €379.00
  OriGene Technologies, Inc.

ERBB4 / HER4 (Internal Region) antibody

Anti-ERBB4 antibody IHC of human glomerulus and renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bt, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

ERBB4 / HER4 (Internal Region) antibody

Anti-ERBB4 antibody IHC of human glomerulus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bt, Chk, Eq, Hst, Hu, Mky, Ms, Op, Por, Prim, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

ERBB4 / HER4 (C-term) antibody

Anti-ERBB4 antibody IHC of human glomerulus and renal tubular epithelium in cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

ERBB4 / HER4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (RC220449, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4. Mouse IgG2b OTI1D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERBB4 / HER4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (RC220449, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4. Mouse IgG1 OTI3A10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERBB4 / HER4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB4 (RC220500, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB4. Mouse IgG1 OTI1E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERBB4 / HER4 (1230-1250) (incl. pos. control) antibody

Detection of endogenous erbB4: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB4-6C5 (0.5 µg/ ml) for 1h at 15-22°C and developed by ECL (exp. time: 30 sec). 
Lane 1: MDA-MB 231 
Lane 2: MDA-MB 468 
Lane 3: MCF-7 
Lane 4: T47D 
Lane 5: SW480 
Lane 6: SW620 Mouse IgG1 6C5 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

ERBB4 / HER4 pTyr1242 (incl. pos. Control) antibody

erbB4 activation Serum starved MDA-MB468 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB4 - 4C6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF; lane 6: 2h EGF lane 7: 4h EGF; lane 8: 8h EGF Mouse IgG1 4C6 Purified Hu WB
0.1 mg / €430.00
  Acris Antibodies GmbH

ERBB4 / HER4 Control Peptide

ERBB4 / HER4
0.25 mg / €250.00
  Acris Antibodies GmbH

ERBB4 / HER4 antibody

ERBB4 / HER4 Rabbit Aff - Purified Hu, Ms, Rt IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ERBB4 / HER4 pTyr1162 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ERBB4 / HER4 pTyr1188 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ERBB4 / HER4 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ERBB4 / HER4 antibody

Immunohistochemistry analyzes of ErbB-4 antibody (Cat.-No.: AP20335PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 mg / €350.00
  Acris Antibodies GmbH

ERBB4 / HER4 pTyr1162 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ERBB4 (phospho Y1162) polyclonal antibody ( Cat # PAB0486 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

ERBB4 / HER4 pTyr1188 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ERBB4 (phospho Y1188) polyclonal antibody ( Cat # PAB0487 ) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu P, WB
0.4 ml / €400.00
  Abnova Taiwan Corp.

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