ERK2 antibody

Principal name

ERK2 antibody

Alternative names for ERK2 antibody

Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1, Extracellular signal-regulated kinase 2

SwissProt ID

P28482 (Human), P46196 (Bovin), P63085 (Mouse), P63086 (Rat)

Gene ID

5594 (MAPK1), 116590 (Mapk1), 26413

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Ye (Yeast), Rb (Rabbit), Bov (Bovine), Mky (Monkey), Chk (Chicken), Sh (Sheep), Gt (Goat), Por (Porcine), Ze (Zebrafish), Chimp (Chimpanzee), Xen (Xenopus)

Available hosts

Rabbit, Chicken, Mouse, Goat, Sheep

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Dot blot (Dot), Flow Cytometry (F), Luminex (LMNX)

Background of ERK2 antibody

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.

General readings

Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT. EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietin-promoted differentiation of astrocytes. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1087-95. PMID: 15249201

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERK1 / ERK2 pTyr205/222 antibody

Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody AP55841PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

ERK1 / ERK2 pTyr205/222 antibody

Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody AP55841PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

ERK2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500468) Mouse IgG2a OTI6F8 Purified Can, Hu, Mky, Rt F, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 (N-term) (incl. pos. control) antibody

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with  MAPK2 antibody (0.5 μg/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431 
Lane 2:SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA231 
Lane 7: T47D Mouse IgG1 6H3 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ERK2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500467) Mouse IgG1 OTI9E9 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500472, Dilution 1:50) Mouse IgG1 OTI6E5 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Anti-MAPK1 mouse monoclonal antibody (TA500473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703). Mouse IgG2a OTI7C9 Purified Can, Hu, Mky, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500475, Dilution 1:50) Mouse IgG2a OTI9H1 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500485) Mouse IgG1 OTI4C2 Purified Can, Hu, Mky, Rt ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Anti-MAPK1 mouse monoclonal antibody (TA500486) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703). Mouse IgG1 OTI7E7 Purified Can, Hu, Mky, Rt ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ERK2 antibody

Detection limit for recombinant GST tagged MAPK1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 1D1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERK2 antibody

Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MAPK1mouse monoclonal antibody. (Dilution 1:50) Mouse 6E5 Aff - Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

ERK2 antibody

Human breast carcinoma stained with anti-ERK-2 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

ERK2 antibody

Human breast carcinoma stained with anti-ERK-2 antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

ERK2 antibody

Human breast carcinoma stained with anti-ERK-2 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

ERK2 antibody

TA302982 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Chimp, Chk, Hu, Ms, Xen WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERK2 antibody

MAPK1 Antibody (Center) (Cat. #TA324692) western blot analysis in MCF-7,HepG2,NCI-H292,Hela,mouse NIH/3T3 and Neuro-2a,rat C6 cell line lysates (35ug/lane).This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow). Mouse IgG1 814CT5.4.2 Purified Bov, Hu, Ms, Rt, Xen WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERK2 (Center) antibody

Western blot analysis of anti-MAPK1 Antibody (Center) Pab (RB11609) in Hela, 293, and HepG2 cell line lysates. MAPK1 Antibody (Center)(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

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