ERK2 antibody

Principal name

ERK2 antibody

Alternative names for ERK2 antibody

Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1, Extracellular signal-regulated kinase 2

SwissProt ID

P28482 (Human), P46196 (Bovin), P63085 (Mouse), P63086 (Rat)

Gene ID

5594 (MAPK1), 116590 (Mapk1), 26413

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ye (Yeast), Rb (Rabbit), Bov (Bovine), Mky (Monkey), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Chimp (Chimpanzee), Xen (Xenopus)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX)

Background of ERK2 antibody

Cell proliferation is regulated in several contexts, for example during development, tissue differentiation, wound healing and immune responses. In mammalian cells, proliferative signals lead to the activation of a protein kinase cascade, resulting in the phosphorylation of two closely related Mitogen-Activated Protein Kinases (MAPK’s) ERK1 and ERK2 of 44 kDa and 42 kDa, respectively. When activated, ERK’s form dimers that translocate to the nucleus where they phosphorylate several classes of transcription factors which are involved in the upregulation of immediate early genes. As such, ERK1 and ERK2 represent a paradigm for a growing family of proline-directed protein kinases that mediate entry, progression and exit from the cell cycle in diverse eukaryotic cells. These enzymes function within highly conserved cascade of sequentially activating protein kinases that transduce signals from diverse extracellular stimuli. Alternative splice transcript variants encoding different protein isoforms have been described. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2. In vertebrates the mitogeninduced sequential activation of the kinases Raf1->Mek1->Erk2->Rsk occurs via the G-protein Ras.

General readings

Lee SM, Nguyen TH, Park MH, Kim KS, Cho KJ, Moon DC, Kim HY, Yoon do Y, Hong JT. EPO receptor-mediated ERK kinase and NF-kappaB activation in erythropoietinpromoted differentiation of astrocytes. Biochem Biophys Res Commun. 2004 Aug 6;320(4):1087-95

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERK1 / ERK2 pTyr205/222 antibody

Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody AP55841PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

ERK1 / ERK2 pTyr205/222 antibody

Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody AP55841PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI6F8 (formerly 6F8)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500468) Mouse IgG2a OTI6F8 Purified Can, Hu, Mky, Rt F, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 (N-term) (incl. pos. control) antibody

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with  MAPK2 antibody (0.5 μg/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431 
Lane 2:SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA231 
Lane 7: T47D Mouse IgG1 6H3 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI9E9 (formerly 9E9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500467) Mouse IgG1 OTI9E9 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)  
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500472, Dilution 1:50) Mouse IgG1 OTI6E5 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7C9 (formerly 7C9)  
Anti-MAPK1 mouse monoclonal antibody (TA500473) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703). Mouse IgG2a OTI7C9 Purified Can, Hu, Mky, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI9H1 (formerly 9H1)  
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500475, Dilution 1:50) Mouse IgG2a OTI9H1 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI4C2 (formerly 4C2)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500485) Mouse IgG1 OTI4C2 Purified Can, Hu, Mky, Rt ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

  anti-MAPK1 (ERK2) mouse monoclonal antibody, clone OTI7E7 (formerly 7E7)  
Anti-MAPK1 mouse monoclonal antibody (TA500486) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK1(RC204703). Mouse IgG1 OTI7E7 Purified Can, Hu, Mky, Rt ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

ERK2 antibody

Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT1A4 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

ERK2 antibody

Detection limit for recombinant GST tagged MAPK1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 1D1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERK2 antibody

  Goat Polyclonal Antibody against MAPK1  
TA302982 (0.5µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Chimp, Chk, Hu, Ms, Xen WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERK2 antibody

  Rabbit Anti-ERK/MAPK (Thr202/Tyr204) Antibody (Phospho-Specific)  
Western blot of human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with λ-Ptase. Rabbit IgG Purified Hu, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERK2 antibody

  Mouse monoclonal Erk2 Antibody  
MAPK1 Antibody (Center) (Cat. #TA324692) western blot analysis in MCF-7,HepG2,NCI-H292,Hela,mouse NIH/3T3 and Neuro-2a,rat C6 cell line lysates (35ug/lane).This demonstrates the MAPK1 antibody detected the MAPK1 protein (arrow). Mouse IgG1 814CT5.4.2 Purified Bov, Hu, Ms, Rt, Xen WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERK2 (Center) antibody

  Rabbit polyclonal MAPK1 Antibody (Center)  
Western blot analysis of anti-MAPK1 Antibody (Center) Pab (RB11609) in Hela, 293, and HepG2 cell line lysates. MAPK1 Antibody (Center)(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERK2 (C-term) antibody

  Rabbit polyclonal MAPK1 Antibody (C-term)  
Western blot analysis of anti-ERK2 C-term Pab (Cat. #TA324942) in UV-treated HL-60 cell lysate. ERK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ERK2 antibody

  Rabbit polyclonal p42 MAPK antibody  
Western blot analysis of extracts from Jurkat cells and 293 cells, using p42 MAPK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERK2 antibody

  Rabbit polyclonal MAPK1/3 (Ab-205/222) antibody  
Western blot analysis of extracts from NIH-3T3 cells, using MAPK1/3 (Ab-205/222) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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