ERN1 / IRE1 antibody

Principal name

ERN1 / IRE1 antibody

Alternative names for ERN1 / IRE1 antibody

Inositol-requiring protein 1, IRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1

SwissProt ID

O75460 (Human), Q9EQY0 (Mouse)

Gene ID

2081 (ERN1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Por (Porcine), Prim (Primate), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)

Background of ERN1 / IRE1 antibody

Unfolded protein response (UPR) sigling, mechanism used by eukaryotic cells to cope ER stress, is initiated by three ER-localized protein sensors: PERK (PKR-like ER kise), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptiol activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of sigling to increase production of molecular chaperones involved in protein folding; and activation of sigling that leads to targeting of misfolded proteins in ER for ubiquitition and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kise as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-termil domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mR to generate a new C-terminus, converting it into a potent UPR transcriptiol activator. IRE1 alpha ictivation in mice has been shown to result in widespread developmental defects, leading to intra-gestatiol embryonic lethality and ER stress mediated cell death is responsible for several pathologies.

General readings

Yokouchi M, Hiramatsu N, Hayakawa K, Okamura M, Du S, Kasai A, Takano Y, Shitamura A, Shimada T, Yao J, Kitamura M.
Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response.
J Biol Chem. 2007 Dec 17
PMID: 18086661

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERN1 / IRE1 (282-433) antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 antibody Cat.-No AM06424SU-N with DAB staining. Mouse IgG1 9F2 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 8D12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERN1 / IRE1 antibody

TA305857 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 (C-term) antibody

Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 (N-term) antibody

Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 pSer274 antibody

Western Blot: IRE1 alpha [p Ser724] Antibody [TA336286] - Detection of phosphorylated Ire1 alpha in Min6 cells, treated with glucose for 3 hours, using NB 100-2323. Rabbit Purified Hu, Ms, Rb, Rt C, E, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

Western Blot: IRE1 alpha Antibody [TA336287] - Detection of Ire1 alpha in transfected Min6 cells. Rabbit Purified Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

Western Blot: IRE1 alpha Antibody [TA336536] - Detection of IRE-1 alpha using TA336536. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

Human Prostate, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3D1 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

Western blot analysis of whole cell lysate from A-20 mouse B cell lymphoma cells probed with Rabbit anti Human IRE1p (AP05555PU-N) at 0.5(A), 1(B) and 2(C) µg/ml Rabbit IgG Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

Western blot analysis of whole cell lysate from A-20 mouse B cell lymphoma cells probed with Rabbit anti Human IRE1p (AP05555PU-N) at 0.5(A), 1(B) and 2(C) µg/ml Rabbit IgG Purified Hu, Ms, Rt C, ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH

ERN1 / IRE1 Control Peptide

ERN1 / IRE1
50 µg / €160.00
  Acris Antibodies GmbH

ERN1 / IRE1 Control Peptide

ERN1 / IRE1
50 µg / €160.00
  Acris Antibodies GmbH

ERN1 / IRE1 Control Peptide

ERN1 / IRE1
50 µg / €160.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

ERN1 / IRE1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ERN1 / IRE1 antibody

ERN1 monoclonal antibody (M01), clone 4D12 Western Blot analysis of ERN1 expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 4D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERN1 / IRE1 antibody

Detection limit for recombinant GST tagged ERN1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1F12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERN1 / IRE1 antibody

ERN1 monoclonal antibody (M05), clone 4E1 Western Blot analysis of ERN1 expression in HeLa ( Cat # L013V1 ). Mouse IgG1 4E1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERN1 / IRE1 antibody

Immunoperoxidase of monoclonal antibody to ERN1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1.5 ug/ml] Mouse IgG1 3D1 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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