ERN1 / IRE1 antibody

Principal name

ERN1 / IRE1 antibody

Alternative names for ERN1 / IRE1 antibody

Inositol-requiring protein 1, IRE1p, Ire1-alpha, IRE1a, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1

SwissProt ID

O75460 (Human), Q9EQY0 (Mouse)

Gene ID

2081 (ERN1), 78943 (Ern1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Rb (Rabbit), Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of ERN1 / IRE1 antibody

Unfolded protein response (UPR) sigling, mechanism used by eukaryotic cells to cope ER stress, is initiated by three ER-localized protein sensors: PERK (PKR-like ER kise), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptiol activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of sigling to increase production of molecular chaperones involved in protein folding; and activation of sigling that leads to targeting of misfolded proteins in ER for ubiquitition and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kise as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-termil domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mR to generate a new C-terminus, converting it into a potent UPR transcriptiol activator. IRE1 alpha ictivation in mice has been shown to result in widespread developmental defects, leading to intra-gestatiol embryonic lethality and ER stress mediated cell death is responsible for several pathologies.

General readings

Yokouchi M, Hiramatsu N, Hayakawa K, Okamura M, Du S, Kasai A, Takano Y, Shitamura A, Shimada T, Yao J, Kitamura M.
Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response.
J Biol Chem. 2007 Dec 17
PMID: 18086661

  1. Quick-Filters:
  2. Highly Validated (1)

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERN1 / IRE1 (282-433) antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 antibody Cat.-No AM06424SU-N with DAB staining. Mouse IgG1 9F2 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

  Goat Anti-ERN1 / IRE1a Antibody  
TA305857 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 (C-term) antibody

  Rabbit Polyclonal IRE1p Antibody  
Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 (N-term) antibody

  Rabbit Polyclonal IRE1p Antibody  
Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

  Rabbit Polyclonal IRE1p Antibody  
Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1 and (B) 2 µg/ml. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 pSer274 antibody

  Rabbit Polyclonal IRE1 alpha [p Ser724] Antibody  
Western Blot: IRE1 alpha [p Ser724] Antibody [TA336286] - Detection of phosphorylated Ire1 alpha in Min6 cells, treated with glucose for 3 hours, using NB 100-2323. Rabbit Purified Hu, Ms, Rb, Rt C, E, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

  Rabbit Polyclonal IRE1 alpha Antibody  
Western Blot: IRE1 alpha Antibody [TA336287] - Detection of Ire1 alpha in transfected Min6 cells. Rabbit Purified Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

  Rabbit Polyclonal IRE1 alpha Antibody  
Western Blot: IRE1 alpha Antibody [TA336536] - Detection of IRE-1 alpha using TA336536. Lane 1: COS-7 untransfected Lane 2: COS-7 expressing wild-type IRE1 alpha Lane 3: COS-7 expressing kinase-dead IRE-1 alpha Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERN1 / IRE1 antibody

Human Prostate, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3D1 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

Western blot analysis of whole cell lysate from A-20 mouse B cell lymphoma cells probed with Rabbit anti Human IRE1p (AP05555PU-N) at 0.5(A), 1(B) and 2(C) µg/ml Rabbit IgG Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ERN1 / IRE1 antibody

Western blot analysis of whole cell lysate from A-20 mouse B cell lymphoma cells probed with Rabbit anti Human IRE1p (AP05555PU-N) at 0.5(A), 1(B) and 2(C) µg/ml Rabbit IgG Purified Hu, Ms, Rt C, ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Ern1 - mouse gene knockout kit via CRISPR

Ern1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ern1 - mouse gene knockout kit via CRISPR

Ern1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ern1 - mouse gene knockout kit via CRISPR

Ern1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ern1 - mouse gene knockout kit via CRISPR

Ern1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ERN1 / IRE1

  Recombinant protein of human endoplasmic reticulum to nucleus signaling 1 (ERN1)  
ERN1 / IRE1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ERN1 overexpression lysate

ERN1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ERN1 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1), 200 uL, >10^7 TU/mL

Lenti ORF particles, ERN1 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ern1 (GFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ern1 (GFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), 200 uL, >10^7 TU/mL  
200 µl / €1,740.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ern1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ern1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), 200 uL, >10^7 TU/mL  
200 µl / €1,570.00
  OriGene Technologies, Inc.

20 Item(s)

per page
  • LinkedIn