ERO1L antibody

Principal name

ERO1L antibody

Alternative names for ERO1L antibody

ERO1-like protein alpha, ERO1-L-alpha, ERO1-L, Oxidoreductin-1-L-alpha, Endoplasmic oxidoreductin-1-like protein

SwissProt ID

A5PJN2 (Bovin), B6CVD7 (Pig), Q6DD71 (Xenla), Q8R180 (Mouse), Q8R4A1 (Rat), Q96HE7 (Human)

Gene ID

30001 (ERO1L)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of ERO1L antibody

Perhaps the most distinctive feature of protein folding in the ER is the abundance of disulfide bonds that must form during maturation of proteins traveling along the secretory pathway. Formation of disulfide bonds is a redox reaction. Thus, to match the flux of disulfide bonds that exit from the ER by virtue of protein secretion, a flux of oxidizing equivalents into the ER is required. In eukaryotic cells, the essential protein relay supporting this flux, and hence disulfide bond formation, involves endoplasmic reticulum oxidoreductin 1 (Ero1) and protein disulfide isomerase (PDI). The temporal pattern of hypoxic ERO1-L alpha induction is very similar to that of genes triggered by the hypoxia inducible transcription factor (HIF-1) and is characteristically mimicked by cobalt and by deferoxamine, but is absent in cells with a defective aryl hydrocarbon receptor translocator (ARNT, HIF-1 alpha). We speculate from these findings that the expression of ERO1-L alpha is probably regulated via the HIF-pathway and thus belongs to the family of classic oxygen regulated genes.

General readings

Otsu M., et al. (2006) Antioxid. Redox Signal. 8: 274-282.Gess B., et al. (2003) J Biochem. 270: 2228-2235.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ERO1L antibody

Western Blot: ERO1L Antibody - Western blot analysis of ERO1L in 293 cell lysates using anti-ERO1L. Rabbit Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ERO1L antibody

ERO1L polyclonal antibody (A01), Lot # FAK0060317QCS1. Western Blot analysis of ERO1L expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ERO1L antibody

Immunoperoxidase of monoclonal antibody to ERO1L on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml] Mouse IgG2b 4G3 Purified Hu, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ERO1L antibody

(0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ERO1L antibody

Western Blot analysis of ERO1L expression in transfected 293T cell line (H00030001-T01) by ERO1L MaxPab polyclonal antibody.

Lane 1: ERO1L transfected lysate(51.48 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ERO1L antibody

Western Blot analysis of ERO1L expression in transfected 293T cell line (H00030001-T02) by ERO1L MaxPab polyclonal antibody.

Lane 1: ERO1L transfected lysate(54.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ERO1L antibody

Western blot analysis of ERO1L in an endogenous 293T cell lysate using ERO1L polyclonal antibody ( Cat # PAB12432 ). Rabbit Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ERO1L antibody

ERO1L Rabbit Biotin Hu, Ms, Rt WB
0.1 ml / €360.00
  Novus Biologicals Inc.

ERO1L antibody

ERO1L Rabbit DyLight488 Hu, Ms, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

ERO1L antibody

ERO1L Rabbit HRP Hu, Ms, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

ERO1L antibody

ERO1L Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
25 µl / €160.00
  Novus Biologicals Inc.

ERO1L antibody

Immunohistochemistry-Paraffin: ERO1L Antibody [NBP1-84800] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ERO1L

ERO1L Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ERO1L (24-468, His-tag)

ERO1L Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

ERO1L (24-468, His-tag)

ERO1L Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

ERO1L

ERO1L Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ERO1L

ERO1L Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ERO1L

ERO1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ERO1L

ERO1L Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ERO1L 293T Cell Transient Overexpression Lysate(Denatured)

ERO1L 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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