Erythropoietin / EPO antibody

Principal name

Erythropoietin / EPO antibody

Alternative names for Erythropoietin / EPO antibody

Epoetin

SwissProt ID

P01588 (Human), P07321 (Mouse), P29676 (Rat), P33707 (Canfa), P33708 (Felca), P33709 (Sheep), P48617 (Bovin), P49157 (Pig), Q9GKA2 (Rabit)

Gene ID

2056 (EPO), 13856 (Epo), 24335

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Chicken, Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Functional assay (FN), Paraffin Sections (P), ELISA (capture) (E(capture)), ELISA capture and detection (E(caption)/E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Neutralisation (NEUT)

Background of Erythropoietin / EPO antibody

Erythropoietin (EPO) is a hormone produced primarily by the kidney and is the main regulator of red blood cell production. Its major functions are to promote the differentiation and development of red blood cells and to initiate the production of hemoglobin. EPO acts by binding to a specific erythropoietin receptor (EPOR) present on target cells, the red cell precursors in the bone marrow, stimulating them to transform into mature erythrocytes. Human EPO cDNA encodes a 193 amino acid residue precursor protein that is processed to yield a 165 amino acid residue mature protein. EPO contains one O-linked and three Nlinked glycosylation sites. Glycosylation of EPO is required for EPO biological activities in vivo. Recombinant EPO has been approved for the treatment of anemia associated with chronic renal failure, anemia secondary to AZT treatment of AIDS, and anemia associated with cancer. There is recent evidence that recombinant EPO may be useful for cardioprotection and neuroprotection. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Kristensen, P.L., et al. Eur. J. Endocrinol. 163(3):391-398(2010)
Kim, M.H., et al. Mol. Biol. (Mosk.) 44(4):656-663(2010)
Carraway, M.S., et al. Circ. Res. 106(11):1722-1730(2010)
Shi, Z., et al. Mol. Cancer Res. 8(4):615-626(2010).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 Epo2 Purified Hu E, WB
1 mg / €550.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Western blot analysis of extracts of various cell lines, using EPO antibody. Rabbit IgG Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Erythropoietin / EPO antibody

WB Suggested Anti-EPO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate;EPO is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Erythropoietin / EPO antibody

Western blot analysis of EPO expression in HepG2 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 Epo1 Purified Hu E, WB
1 mg / €550.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Immunofluorescence analysis of GC7901 cells using EPO antibody Cat.-No AM06504SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4F11 Ascites Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Detect EPO antigen (100ng/well) using Mouse anti-Human EPO Antibody Cat.-No AM33423PU-N (clone 85) at 1/1000 dilution (1 µg/ml). Mouse IgG1 85 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG2b 13A180 (1C8) FITC Hu F
100 Tests / €710.00
  Acris Antibodies GmbH

Erythropoietin / EPO (N-term) antibody

Western blot analysis of Erythropoietin / EPO Antibody (N-term) Cat.-No AP51461PU-N in mouse L929 cell line lysates (35ug/lane). This demonstrates the Erythropoietin antibody detected the Erythropoietin protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG2a AE7A5 Purified Hu, Rt WB
0.25 mg / €610.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 6B8 Purified Hu E
0.2 mg / €430.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 BGN/6C2 Purified Hu E
0.2 mg / €430.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 6C7 Purified Hu E
0.2 mg / €430.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Erythropoietin / EPO Rabbit Purified Hu E, FN, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Erythropoietin / EPO antibody

Western Blot analysis of EPO expression in transfected 293T cell line (H00002056-T01) by EPO MaxPab polyclonal antibody.

Lane 1: EPO transfected lysate(21.23 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Erythropoietin / EPO antibody

Western Blot analysis of EPO expression in transfected 293T cell line (H00002056-T01) by EPO MaxPab polyclonal antibody.

Lane 1: EPO transfected lysate(21.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Erythropoietin / EPO antibody

Western Blot analysis of EPO expression in transfected 293T cell line by EPO monoclonal antibody (M01), clone 4G7.

Lane 1: EPO transfected lysate(21.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 4G7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG2a 4G7 Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 1B12 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Erythropoietin / EPO antibody

Erythropoietin / EPO Mouse IgG1 Epo1 E, WB
0.2 mg / €470.00
  Abnova Taiwan Corp.

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