Erythropoietin receptor antibody

Principal name

Erythropoietin receptor antibody

Alternative names for Erythropoietin receptor antibody

EPOR, EPO-R

SwissProt ID

P14753 (Mouse), P19235 (Human), Q07303 (Rat), Q2KL21 (Canfa), Q4W815 (Xenla), Q9MYZ9 (Pig)

Gene ID

2057 (EPOR), 13857 (Epor)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Sh (Sheep), Eq (Equine), GP (Guinea Pig), Bov (Bovine), Rb (Rabbit)

Available hosts

Mouse, Goat, Sheep, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of Erythropoietin receptor antibody

Recombinant Human Erythropoietin Receptor (EPO R) is a type I membrane-spanning protein, expressed in a mouse myeloma cell line, NSO. This product is produced from a cDNA sequence encoding the extracellular domain of the EPO receptor. The 225 amino acid residue mature recombinant soluble EPO receptor, generated after cleavage of a 25 amino acid residue signal peptide, has predicted molecular mass of approximately 25 kDa. From glycosylation, recombinant human EPO receptor migrates as an approximately 32 kDa protein in SDS-PAGE.Erythropoietin (EPO), a glycoprotein produced primarily by the kidney and at lower levels by the liver, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. The biological effects of EPO are mediated by the erythropoietin receptor (EPO R). The EPO receptor belongs to the cytokine receptor superfamily, which includes the receptors for granulocyte-macrophage colony-stimulating factor (GMCSF), granulocyte colony-stimulating factor (G-CSF), IL2, IL3, IL4, IL5, IL6, IL7, LIF, and others.These receptors contain four conserved cysteines and a Trp-Ser-X-Trp-Ser (WSXWS) motif in their extracellular domains, where X represents any amino acid. The EPO receptor differs from the other members of the cytokine receptor superfamily in that the EPO receptor can be stimulated to signal cell growth by binding either EPO or gp55. Human and mouse EPO receptor share 82 % identity in their amino acid sequence. Truncated and soluble forms of the EPO receptor result from alternative splicing of the EPO receptor gene. The gene for human EPO receptor has been mapped to the p region of chromosome 19. Recombinant EPO receptor binds EPO with high affinity and is a potent EPO antagonist. When EPO is present at low concentrations, the EPO receptor initiates prolongation of G1 of the cell cycle and sends a differentiation signal; whereas at high EPO concentrations, a proliferation signal is generated and the G1 is shortened. EPO binding induces the stimulation of Jak2 tyrosine kinase, which leads to tyrosine phosphorylation of several proteins, including the EPO receptor, resulting in the activation of intracellular pathways (Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors). Recombinant Human Erythropoietin Receptor is expressed in megakaryocytes, erythoid progenitors, endothelial cells, and, possibly, neurons. It has also been found in human sera. Expression of the EPO receptor in normally IL-3-dependent cell lines such as Ba/F3 (pro-B lymphocyte cell line) or 32D (myeloid progenitor cell line) allows cell proliferation in the presence of either EPO or IL3, indicating that the EPO receptor is capable of generating a proliferative signal in these cells.

General readings

Nakatsuji T. Long-term survival of a piece of rat bone marrow graft with the bone in syngeneic rat spleen: new roles of bone marrow mast cells and host spleen megakaryocytes secreting vascular endothelial growth factor (VEGF). Haema 2005; 8(3):450-457
Fairchild Benyo D, Conrad KP. Expression of the erythropoietin receptor by trophoblast cellsin the human placenta. Biol Reprod. 1999 Apr;60(4):861-70
Harris KW, Mitchell RA, Winkelmann JC. Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli. J Biol Chem. 1992 Jul 25;267(21):15205-9

  1. Quick-Filters:
  2. Highly Validated (1)

Items 1 to 20 of 44 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Erythropoietin receptor antibody

Western Blot analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.

Lane 1: EPOR transfected lysate(55.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 3D10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Erythropoietin receptor antibody

Western blot analysis of extracts from JurKat cells, using Epo-R (Ab-426) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Western blot analysis of A549 cell using TA323735(EPOR Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA323736(EPOR Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Western blot analysis of Epo-R expression in EPO treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Western blot analysis of extracts of various cell lines, using EPOR antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

WB Suggested Anti-EPOR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

WB Suggested Anti-Epor Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Liver Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Western Blot: EPO Receptor Antibody [TA336737] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml. Rabbit Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Erythropoietin receptor antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EPOR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804627) Mouse IgG2a OTI4F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Erythropoietin receptor antibody

CGE analysis of purified VP-2E8 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 μg of purified VP-2E8-B6 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).(240 kDa / 7 kDa / 4,5 kDa). Mouse IgG1 VP-2E8 Purified Hu E, F, IP
0.1 mg / €330.00
  Acris Antibodies GmbH

Erythropoietin receptor (Cytoplasm. Dom.) antibody

Mass spectroscopic analysis of EpoR immuno - precipitated from UT7 cells by AM26787PU-N or other antibody that were further analysed on denaturing SDS - PAGE gels (identified in Western blot with AM26787PU-N). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5. Rat IgG2b BCO-3H2 Purified Hu E, F, WB
0.1 mg / €330.00
  Acris Antibodies GmbH

Erythropoietin receptor antibody

Western blot analysis of EpoR antibody (AP20227PU-N) in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Erythropoietin receptor (Center) antibody

Western blot analysis of Erythropoietin receptor Antibody Cat.-No AP51446PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the EPOR antibody detected the EPOR protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Erythropoietin receptor pTyr368 antibody

Western blot analysis of extracts from K562 tissue using Epo-R (Phospho-Tyr368) antibody AP55943PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Erythropoietin receptor pTyr368 antibody

Western blot analysis of extracts from K562 tissue using Epo-R (Phospho-Tyr368) antibody AP55943PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

Erythropoietin receptor antibody

Erythropoietin receptor Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Erythropoietin receptor antibody

Western Blot analysis of EPOR expression in transfected 293T cell line (H00002057-T01) by EPOR MaxPab polyclonal antibody.

Lane 1: EPOR transfected lysate(55.88 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Erythropoietin receptor antibody

EPOR MaxPab rabbit polyclonal antibody. Western Blot analysis of EPOR expression in human liver. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Erythropoietin receptor antibody

EPOR monoclonal antibody (M02), clone 3F6. Western Blot analysis of EPOR expression in HeLa ( Cat # L013V1 ). Mouse IgG2b 3F6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Items 1 to 20 of 44 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn