ETF1 / ERF1 antibody

Principal name

ETF1 / ERF1 antibody

Alternative names for ETF1 / ERF1 antibody

Eukaryotic release factor 1, TB3-1, Protein Cl1, RF1, SUP45L1, Eukaryotic peptide chain release factor subunit 1

SwissProt ID

P35615 (Xenla), P62495 (Human), P62496 (Mesau), Q0VCX5 (Bovin), Q8BWY3 (Mouse)

Gene ID

2107 (ETF1), 4921078 (IPR001365)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Flow Cytometry (F)

Background of ETF1 / ERF1 antibody

Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the “GTP state,” it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the “GDP state,” it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ETF1 / ERF1 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ETF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200) Mouse IgG2a OTI2E3 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ETF1 / ERF1 antibody

WB Suggested Anti-ETF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ETF1 / ERF1 antibody

ETF1 monoclonal antibody (M02), clone 2H4. Western Blot analysis of ETF1 expression in HepG2 ( Cat # L019V1 ). Mouse IgG2b 2H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ETF1 / ERF1 antibody

ETF1 / ERF1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ETF1 / ERF1 antibody

ELISA: ERF1 Antibody [27010002] Rabbit Hu E, P, WB
50 µg / €440.00
  Novus Biologicals Inc.

ETF1 / ERF1 antibody

Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using eRF1(NBP1-31113) antibody at 1:100 dilution. Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ETF1 / ERF1 antibody

Western Blot: eRF1 Antibody [NBP1-31144] - Western blot analysis of eRF1 Antibody on: A: Raji 10% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ETF1 / ERF1 antibody

ETF1 / ERF1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ETF1 / ERF1

ETF1 / ERF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ETF1 overexpression lysate

ETF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

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