ETF1 / ERF1 antibody

Principal name

ETF1 / ERF1 antibody

Alternative names for ETF1 / ERF1 antibody

Eukaryotic release factor 1, TB3-1, Protein Cl1, RF1, SUP45L1, Eukaryotic peptide chain release factor subunit 1

SwissProt ID

P35615 (Xenla), P62495 (Human), P62496 (Mesau), Q0VCX5 (Bovin), Q8BWY3 (Mouse)

Gene ID

2107 (ETF1), 225363 (Etf1)

Available reactivities

Hu (Human), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rt (Rat), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F)

Background of ETF1 / ERF1 antibody

Termition of protein biosynthesis and release of the scent polypeptide chain are sigled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termition is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tR, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functiol counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).

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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ETF1 / ERF1 antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ETF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200) Mouse IgG2a OTI2E3 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

ETF1 / ERF1 antibody

WB Suggested Anti-ETF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ETF1 / ERF1 antibody

ETF1 monoclonal antibody (M02), clone 2H4. Western Blot analysis of ETF1 expression in HepG2 ( Cat # L019V1 ). Mouse IgG2b 2H4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ETF1 / ERF1 antibody

ETF1 / ERF1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Etf1 - mouse gene knockout kit via CRISPR

Etf1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Etf1 - mouse gene knockout kit via CRISPR

Etf1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Etf1 - mouse gene knockout kit via CRISPR

Etf1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Etf1 - mouse gene knockout kit via CRISPR

Etf1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ETF1 / ERF1

ETF1 / ERF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ETF1 / ERF1

ETF1 / ERF1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ETF1 overexpression lysate

ETF1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ETF1 (mGFP-tagged) - Human eukaryotic translation termination factor 1 (ETF1), 200 uL, >10^7 TU/mL

Lenti ORF particles, ETF1 (mGFP-tagged) - Human eukaryotic translation termination factor 1 (ETF1), 200 uL, >10^7 TU/mL  
200 µl / €1,040.00
  OriGene Technologies, Inc.

Lenti ORF particles, ETF1 (Myc-DDK tagged) - Human eukaryotic translation termination factor 1 (ETF1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, ETF1 (Myc-DDK tagged) - Human eukaryotic translation termination factor 1 (ETF1) , 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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