ETFA antibody

Principal name

ETFA antibody

Alternative names for ETFA antibody

Alpha-ETF, Electron transfer flavoprotein subunit alpha

SwissProt ID

P13803 (Rat), P13804 (Human), Q2KJE4 (Bovin), Q99LC5 (Mouse)

Gene ID

2108 (ETFA), 110842 (Etfa), 300726

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Dros (Drosophila), Ms (Mouse), Por (Porcine), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Rb (Rabbit), Eq (Equine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)

Background of ETFA antibody

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

General readings

Ohkuma, A., et al. Muscle Nerve 39(3):333-342(2009)
Chiong, M.A., et al. Mol. Genet. Metab. 92 (1-2), 109-114 (2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Schiff, M., et al. Mol. Genet. Metab. 88(2):153-158(2006).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ETFA antibody

  Rabbit Polyclonal Anti-ETFA Antibody  
WB Suggested Anti-ETFA Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ETFA (C-term) antibody

AP21285PU-N ETFA antibody
staining of Human Heart lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ETFA (139-152) antibody

  Goat Anti-ETFA (aa139-152) Antibody  
(0.1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ETFA antibody

  Rabbit Polyclonal Anti-ETFA Antibody  
WB Suggested Anti-ETFA Antibody Titration: 0.2-1 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

ETFA antibody

  ETFA mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETFA (RC202147, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETFA.(1:2000) Mouse IgG1 OTI5C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ETFA (C-term) antibody

Formalin fixed and paraffin embedded human skeletal muscle reacted with ETFA Antibody (C-term) Cat.-No AP51468PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ETFA - human gene knockout kit via CRISPR

ETFA - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ETFA - human gene knockout kit via CRISPR

ETFA - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ETFA - human gene knockout kit via CRISPR

ETFA - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Etfa - mouse gene knockout kit via CRISPR

Etfa - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Etfa - mouse gene knockout kit via CRISPR

Etfa - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Etfa - mouse gene knockout kit via CRISPR

Etfa - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Etfa - mouse gene knockout kit via CRISPR

Etfa - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ETFA (full length, N-term HIS tag, transcript variant 1)

  Recombinant protein of human electron-transfer-flavoprotein, alpha polypeptide (ETFA), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
ETFA Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ETFA overexpression lysate

ETFA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ETFA overexpression lysate

ETFA overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ETFA (mGFP-tagged) - Human electron-transfer-flavoprotein, alpha polypeptide (ETFA), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ETFA (mGFP-tagged) - Human electron-transfer-flavoprotein, alpha polypeptide (ETFA), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, ETFA (Myc-DDK tagged) - Human electron-transfer-flavoprotein, alpha polypeptide (ETFA), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ETFA (Myc-DDK tagged) - Human electron-transfer-flavoprotein, alpha polypeptide (ETFA), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Etfa (GFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, Etfa (GFP-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL

Lenti ORF particles, Etfa (Myc-DDK-tagged) - Mouse electron transferring flavoprotein, alpha polypeptide (Etfa), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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