ETV4 / PEA3 antibody

Principal name

ETV4 / PEA3 antibody

Alternative names for ETV4 / PEA3 antibody

E1AF, E1A-F, ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, Polyomavirus enhancer activator 3 homolog

SwissProt ID

P28322 (Mouse), P43268 (Human)

Gene ID

2118 (ETV4), 4211802 (accD)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ETV4 / PEA3 antibody

Ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-α. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears thatall of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of whichis expressed primarily in cells of hematopoietic origin.

General readings

Span,P.N., et al., (2003) Breast Cancer Res. Treat. 79 (1), 129-131

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ETV4 / PEA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ETV4 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ETV4 / PEA3 antibody

WB Suggested Anti-ETV4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Small Intestine Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ETV4 / PEA3 antibody

Western blot analysis of extracts of various cell lines, using ETV4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ETV4 / PEA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ETV4 (RC215093, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ETV4. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ETV4 / PEA3 antibody

Western blot analysis using ETV4 antibody Cat.-No AM06250SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY ETV4 cDNA (Lane 2). Mouse IgG1 1A2G3 Ascites Hu WB
0.1 ml / €440.00
  Acris Antibodies GmbH

ETV4 / PEA3 (50-109) antibody

Placenta, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1A2G3 Ascites Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ETV4 / PEA3 (C-term) antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ETV4 / PEA3 antibody

Western Blot analysis of ETV4 expression in transfected 293T cell line by ETV4 monoclonal antibody (M01), clone 3G9-1B9.

Lane 1: ETV4 transfected lysate(54 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 3G9-1B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ETV4 / PEA3 antibody

ETV4 / PEA3 Mouse IgG2a 1A3-1D3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ETV4 / PEA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ETV4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
50 µg / €205.00
  Assay Biotech

ETV4 / PEA3 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ETV4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P
0.1 mg / €310.00
  Assay Biotech

ETV4 / PEA3 antibody

Human Small Intestine; WB Suggested Anti-ETV4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Small Intestine; ETV4 antibody - middle region (ARP32263_P050) in Human Small Intestine cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ETV4 / PEA3 antibody

ETV4 / PEA3 Rabbit Hu E, IP, WB
50 µg / €470.00
  Novus Biologicals Inc.

ETV4 / PEA3 antibody

Western blot : Pea3 Antibody (1A2G3)[NBP1-28856]- analysis using anti-ETV4 mAb against K562 cell lysate (1). Mouse IgG1 1A2G3 Ascites Hu E, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ETV4 / PEA3 antibody

Immunohistochemistry-Paraffin: Pea3 Antibody [NBP1-87960] - Immunohistochemical staining of human cerebral cortex shows strong nuclear(nucleolar) positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ETV4 / PEA3 (full length, N-term HIS tag, transcript variant 2)

ETV4 / PEA3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

ETV4 / PEA3

ETV4 / PEA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ETV4 / PEA3

ETV4 / PEA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ETV4 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)

ETV4 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)  
0.1 mg / €270.00
  Abnova Taiwan Corp.

ETV4 overexpression lysate

ETV4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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