EWSR1 antibody
Principal name
EWSR1 antibody
Alternative names for EWSR1 antibody
EWSR-1, EWS, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein
Ncbi ID
9606, AAK11227, NP_005234, NP_001156757, NP_001156757.1, NM_005243, NM_001163286, NM_013986, NM_007968
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of EWSR1 antibody
This gene encodes a multifunctiol protein that is involved in various cellular processes, including gene expression, cell sigling, and R processing and transport. The protein includes an N-termil transcriptiol activation domain and a C-termil R-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-termil transcriptiol activation domain of this protein fused to the C-termil D-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Altertive splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
General readings
Toretsky JA, Erkizan V, Levenson A, Abaan OD, Parvin JD, Cripe TP, Rice AM, Lee SB, Uren A.
Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A.
Cancer Res. 2006 Jun 1;66(11):5574-81.
PMID: 16740692
Immunoperoxidase of monoclonal antibody to EWSR1 on formalin-fixed paraffin-embedded human urinary bladder. [antibody concentration 3 ug/ml]
Western Blot analysis of EWSR1 expression in transfected 293T cell line by EWSR1 monoclonal antibody (M01), clone 5C10.Lane 1: EWSR1 transfected lysate(68.5 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged EWSR1 is approximately 0.03ng/ml as a capture antibody.
EWSR1 polyclonal antibody (A01), Lot # 050921JC01 Western Blot analysis of EWSR1 expression in HeLa ( Cat # L013V1 ).
Human Jurkat; WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; EWSR1 antibody - N-terminal region (ARP40750_P050) in Human Jurkat cells using Western Blot
EWSR1 polyclonal antibody ( Cat # PAB11611 ) ( 0.1 µg/ml ) staining of HeLa nuclear extract ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
Human HeLa; . Lanes: Lane 1: 50ug Hela Lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: EWSR1. Submitted by: Archa Fox, University of Western Australia. EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; EWSR1 antibody - middle region (ARP38594_P050) in Human HeLa cells using Western Blot
Human Jurkat; WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate.. EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.; EWSR1 antibody - middle region (ARP38594_P050) in Human Jurkat cells using Western Blot
Human Heart; Rabbit Anti-EWSR1 antibody. Catalog Number: ARP40749 . Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; EWSR1 antibody - middle region (ARP40749_P050) in Human Heart using Immunohistochemistry
Human HeLa; . Lanes: Lane 1: 50ug Hela Lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit-HRP. Secondary Antibody Dilution: 1:10,000. Gene Name: EWSR1. Submitted by: Archa Fox, University of Western Australia. EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; EWSR1 antibody - middle region (ARP40749_P050) in Human HeLa cells using Western Blot
Human; . Lanes: 1. 30 ug MiaPaca-2 cell lysate. 2. 30 ug Panc-1 cell lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit HRP. Secondary Antibody Dilution: 1:4000. Gene Name: EWSR1. Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ.; EWSR1 antibody - middle region (ARP40749_P050) in Human cells using Western Blot
Human HEK293; Sample Type: HEK cells transfected with pFLAG EWSR1-ATF1Primary Dilution: 1:1000. EWSR1 is supported by BioGPS gene expression data to be expressed in HEK293.; EWSR1 antibody - middle region (ARP40749_P050) in Human HEK293 cells using Western Blot
Human HepG2; WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; EWSR1 antibody - middle region (ARP40749_P050) in Human HepG2 cells using Western Blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of EWSR1 expression in transfected 293T cell line by EWSR1 antibody.
Detection limit for recombinant GST tagged EWSR1 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of EWS expression in HepG2 (A); Jurkat (B); HeLa (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EWSR1 (RC203709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EWSR1.
ELISA: EWSR1 Antibody
TA305656 (3.8 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of extracts from HeLa cells, Jurkat cells and K562 cells, using EWSR1 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 68 kDa . Positive control: 293 and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: 293 and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
EWSR1 Antibody (C-term) (Cat. #TA324555) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow).
IF image of Hela cell stained with EWSR1 Antibody (C-term)(Cat#TA324555).Hela cells were incubated with EWSR1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .EWSR1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of EWSR1 Antibody (C-term) (Cat. #TA325075) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab.
IF image of U251 cell stained with EWSR1 Antibody (C-term)(Cat#TA325075).U251 cells were incubated with EWSR1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .EWSR1 immunoreactivity is localized to Nucleus significantly.
EWSR1 Antibody (C-term) (Cat. #TA325075) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate.EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat
Lanes: Lane 1: 50ug Hela Lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 10, 000; Gene Name: EWSR1; Submitted by: Archa Fox, University of Western Australia; EWSR1 is strongly supported by B
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate
Lanes: 1. 30 ug MiaPaca-2 cell lysate; 2. 30 ug Panc-1 cell lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Goat anti-Rabbit HRP; Secondary Antibody Dilution: 1: 4000; Gene Name: EWSR1; Submitted by: Dr. Crissy Dudgeon, Ph.D., CINJ;
Lanes: Lane 1: 50ug Hela Lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit-HRP; Secondary Antibody Dilution: 1: 10, 000; Gene Name: EWSR1; Submitted by: Archa Fox, University of Western Australia; EWSR1 is strongly supported by B
Sample Type: HEK cells transfected with pFLAG EWSR1-ATF1Primary Dilution: 1: 1000EWSR1 is supported by BioGPS gene expression data to be expressed in HEK293
Rabbit Anti-EWSR1 antibody; Paraffin Embedded Tissue: Human Heart cell Cellular Data: cardiac cell of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateEWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA343795 | EWSR1 antibody |
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| Rabbit Polyclonal Anti-EWSR1 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA305656 | EWSR1 antibody |
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| Goat Anti-EWS / EWSR1 Antibody | |||||||
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Goat | Aff - Purified | Bov, Hu, Ms, Por, Rt | E, P, WB |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA312205 | EWSR1 antibody |
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| Rabbit polyclonal anti-EWSR1 antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324001 | EWSR1 antibody |
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| Anti-EWSR1 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324555 | EWSR1 antibody |
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| Rabbit polyclonal EWSR1 Antibody (C-term) | |||||||
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Rabbit | IgG | Purified | Hu, Ms | ICC/IF, WB |
0.4 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325075 | EWSR1 (C-term) antibody |
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| Rabbit polyclonal EWSR1 Antibody (C-term) | |||||||
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Rabbit | Hu | WB |
0.4 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA343522 | EWSR1 antibody |
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| Rabbit Polyclonal Anti-EWSR1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA343796 | EWSR1 antibody |
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| Rabbit Polyclonal Anti-EWSR1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA590199 | EWSR1 antibody |
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| Rabbit Polyclonal EWSR1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| AM31782PU-N | EWSR1 antibody |
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Mouse | IgG2a | 5C10 | Purified | Hu | E, P, WB |
50 µg /
€530.00
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| Acris Antibodies GmbH | |||||||
| +2 additional images |
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN203709 | EWSR1 - human gene knockout kit via CRISPR |
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Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN203709BN | EWSR1 - human gene knockout kit via CRISPR |
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Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203709LP | EWSR1 - human gene knockout kit via CRISPR |
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Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN203709RB | EWSR1 - human gene knockout kit via CRISPR |
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Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303709 | EWSR1 (transcript variant EWS) |
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| Recombinant protein of human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant EWS | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401610 | EWSR1 overexpression lysate |
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0.1 mg /
€280.00
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| OriGene Technologies, Inc. | ||
| LY431514 | EWSR1 overexpression lysate |
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0.1 mg /
€280.00
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| OriGene Technologies, Inc. | ||
| LY432342 | EWSR1 overexpression lysate |
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0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC223794L2V | Lenti ORF particles, EWSR1 (mGFP-tagged) - Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,330.00
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| OriGene Technologies, Inc. | ||
| RC228486L2V | Lenti ORF particles, EWSR1 (mGFP-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 4, 200 uL, >10^7 TU/mL |
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200 µl /
€1,250.00
|
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| OriGene Technologies, Inc. | ||
