EWSR1 antibody

Principal name

EWSR1 antibody

Alternative names for EWSR1 antibody

EWSR-1, EWS, RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein

SwissProt ID

Q01844 (Human), Q61545 (Mouse)

Gene ID

2130 (EWSR1), 14030 (Ewsr1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of EWSR1 antibody

This gene encodes a multifunctiol protein that is involved in various cellular processes, including gene expression, cell sigling, and R processing and transport. The protein includes an N-termil transcriptiol activation domain and a C-termil R-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-termil transcriptiol activation domain of this protein fused to the C-termil D-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Altertive splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

General readings

Toretsky JA, Erkizan V, Levenson A, Abaan OD, Parvin JD, Cripe TP, Rice AM, Lee SB, Uren A.
Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A.
Cancer Res. 2006 Jun 1;66(11):5574-81.
PMID: 16740692

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EWSR1 antibody

  Rabbit Polyclonal Anti-EWSR1 Antibody  
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Goat Anti-EWS / EWSR1 Antibody  
TA305656 (3.8 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Rabbit polyclonal anti-EWSR1 antibody  
Western blot analysis of extracts from HeLa cells, Jurkat cells and K562 cells, using EWSR1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Anti-EWSR1 Rabbit Polyclonal Antibody  
Predicted band size: 68 kDa . Positive control: 293 and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: 293 and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Rabbit polyclonal EWSR1 Antibody (C-term)  
EWSR1 Antibody (C-term) (Cat. #TA324555) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EWSR1 antibody detected the EWSR1 protein (arrow). Rabbit IgG Purified Hu, Ms ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

EWSR1 (C-term) antibody

  Rabbit polyclonal EWSR1 Antibody (C-term)  
Western blot analysis of EWSR1 Antibody (C-term) (Cat. #TA325075) in A2058 cell line lysates (35ug/lane). EWSR1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Rabbit Polyclonal Anti-EWSR1 Antibody  
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate.EWSR1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Rabbit Polyclonal Anti-EWSR1 Antibody  
WB Suggested Anti-EWSR1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateEWSR1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

  Rabbit Polyclonal EWSR1 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EWSR1 (RC203709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EWSR1. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EWSR1 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 5C10 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

EWSR1 - human gene knockout kit via CRISPR

EWSR1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EWSR1 - human gene knockout kit via CRISPR

EWSR1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EWSR1 - human gene knockout kit via CRISPR

EWSR1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EWSR1 - human gene knockout kit via CRISPR

EWSR1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EWSR1 (transcript variant EWS)

  Recombinant protein of human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant EWS  
EWSR1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

EWSR1 overexpression lysate

EWSR1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

EWSR1 overexpression lysate

EWSR1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

EWSR1 overexpression lysate

EWSR1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EWSR1 (mGFP-tagged) - Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, EWSR1 (mGFP-tagged) - Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,330.00
  OriGene Technologies, Inc.

Lenti ORF particles, EWSR1 (mGFP-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, EWSR1 (mGFP-tagged)-Human Ewing sarcoma breakpoint region 1 (EWSR1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,250.00
  OriGene Technologies, Inc.

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