EYA2 / EAB1 antibody

Principal name

EYA2 / EAB1 antibody

Alternative names for EYA2 / EAB1 antibody

Eyes absent homolog 2

SwissProt ID

O00167 (Human), O08575 (Mouse), Q58DB6 (Bovin), Q9H4S9 (Human)

Gene ID

2139 (EYA2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of EYA2 / EAB1 antibody

A gene on chromosome 20q13.1 encodes Eya2 (eyes absent). EYA2 is one of four members of the eyes absent family. A 271 amino acid domain at the carboxy-terminal is highly conserved amongst the members of the eyes absent family, while the PST (proline-serive-threonin)-rich amino-terminal is highly divergent. EYA2 is expressed relatively late in development in the cytoplasm of extensor tendons and ligaments of the phalangeal elements of the limb, cranial placodes, branchial arches, central nervous system, and the developing eye. Pax3 induces the expression of Eya2 in a cascade that is necessary and sufficient for myogenesis. EYA2, like EYA1, acts as a transcriptional activator in connective tissue patterning through its PST domain, which functions as a transactivation domain. EYA2 is translocated to the nucleus by Six proteins, which interact through their domain and homeodomain with EYA2. EYA2 carboxy-terminal interacts with the G Alpha z and G Alphai 2 proteins. This interaction prevents Six proteins from translocating EYA2 to the nucleus.

General readings

Krishnan N., et al. (2009) J. Biol. Chem. 284:16066-16070Fan X., et al. (2000) J. Biol. Chem. 275:32129-32134

10 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EYA2 / EAB1 antibody

Western Blot analysis of EYA2 expression in transfected 293T cell line (H00002139-T01) by EYA2 MaxPab polyclonal antibody.

Lane 1: EYA2 transfected lysate(59.18 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

EYA2 / EAB1 antibody

Western Blot analysis of EYA2 expression in transfected 293T cell line (H00002139-T01) by EYA2 MaxPab polyclonal antibody.

Lane 1: EYA2 transfected lysate(59.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EYA2 / EAB1 antibody

Immunofluorescence of monoclonal antibody to EYA2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2F8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EYA2 / EAB1 antibody

Western Blot: EYA2 Antibody [NBP1-46816] - Detection of Human EYA2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody used for IP at 3 mcg/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated EYA2, was used at 1 mcg/ml, and the Reagents and Procedures  were incorporated. Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit Aff - Purified Hu IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

EYA2 / EAB1 antibody

Western Blot: EYA2 Antibody [NBP1-46817] - Detection of Human EYA2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody A302-688A used for WB at 0.2 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody A302-687A, which recognizes an upstream epitope. For blotting immunoprecipitated EYA2, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

EYA2 / EAB1 antibody

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-84026] - Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EYA2 / EAB1 (244-514, His-tag)

EYA2 / EAB1 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

EYA2 / EAB1 (244-514, His-tag)

EYA2 / EAB1 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

EYA2 / EAB1

EYA2 / EAB1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

EYA2 293T Cell Transient Overexpression Lysate(Denatured)

EYA2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

10 Item(s)

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