EYA3 antibody

Principal name

EYA3 antibody

Alternative names for EYA3 antibody

Eyes absent homolog 3

SwissProt ID

P97480 (Mouse), Q99504 (Human), Q9YH99 (Chick)

Gene ID

2140 (EYA3)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of EYA3 antibody

EYA3 is a tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. This protein promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. EYA3 coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity.

General readings

Borsani,G., et al., (1999) Hum.Mol.Genet.8(1),38679

8 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EYA3 antibody

WB Suggested Anti-EYA3 Antibody Titration: 0.05-1.0ug/ml; Positive Control: HepG2 cell lysate Rabbit Aff - Purified Can, Hu, Ms, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EYA3 antibody

Host: Rabbit; Target Name: EYA3; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

EYA3 antibody

EYA3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

EYA3 antibody

Human Skeletal Muscle; EYA3 antibody - middle region (ARP32331_P050) in Human Skeletal Muscle cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

EYA3 antibody

Western Blot: EYA3 Antibody [NBP1-46818] - Detection of Human EYA3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- EYA3 antibody A302-689A used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody A302-690A, which recognizes a downstream epitope. For blotting immunoprecipitated EYA3, the ReliaBLOT® Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B). Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

EYA3 antibody

Immunoprecipitation: EYA3 Antibody [NBP1-46819] - Detection of Human EYA3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA3 antibody used for IP at 3 mcg/mg lysate. EYA3 was also immunoprecipitated by rabbit anti-EYA3 antibody, which recognizes an upstream epitope. For blotting immunoprecipitated EYA3, was used at 0.4 mcg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EYA3

EYA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

EYA3

EYA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

8 Item(s)

per page
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