EZH1 antibody

Principal name

EZH1 antibody

Alternative names for EZH1 antibody

ENX-2, KIAA0388, Histone-lysine N-methyltransferase EZH1, Enhancer of zeste homolog 1

SwissProt ID

A7E2Z2 (Bovin), P70351 (Mouse), Q92800 (Human)

Gene ID

2145 (EZH1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of EZH1 antibody

Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression.(2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).

General readings

Abel KJ, Brody LC, Valdes JM, Erdos MR, McKinley DR, Castilla LH, Merajver SD, Couch FJ, Friedman LS, Ostermeyer EA, Lynch ED, King MC, Welcsh PL, Osborne-Lawrence S, Spillman M, Bowcock AM, Collins FS, Weber BL.
Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.
Genomics. 1996 Oct 15;37(2):161-71.
PMID: 8921387

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EZH1 (N-term) antibody

TA302843 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Western blot analysis of extracts from HT-29 cells, LOVO cells, A549 cells, NIH-3T3 cells, RAW264.7 cells and COS-7 cells, using EZH1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EZH1 (N-term) antibody

Western blot analysis of EZH1 in mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 ug/mL Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Western blot analysis of extracts of various cell lines, using EZH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysateThere is BioGPS gene expression data showing that EZH1 is expressed in COLO205 Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Western Blot: EZH1 Antibody [TA336341] - 15 ug of HeLa nuclear extract on PVDF membrane. Image provided by verified customer. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Western blot analysis of EZH1 expression in A549 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Immunohistochemistry (IHC) analyzes of ENX-2 antibody (Cat.-No.: AP06748PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EZH1 (Center) antibody

(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. RIGHT) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with EZH1 Antibody (Center) (Cat.#AP12228PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EZH1 control peptide

EZH1
50 µg / €160.00
  Acris Antibodies GmbH

EZH1 antibody

Western blot detection of EZH1 in mouse ( lane 1 ) and human ( lane 2 ) spleen lysate using EZH1 polyclonal antibody ( Cat # PAB0192 ) at 2 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

EZH1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with EZH1 polyclonal antibody ( Cat # PAB4353 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma . Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

EZH1 antibody

EZH1 polyclonal antibody ( Cat # PAB6289 ) staining ( 2 µg/ml ) of MOLT 4 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

EZH1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using EZH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

EZH1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using EZH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

EZH1 antibody

Human Brain; WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; EZH1 antibody - N-terminal region (ARP34235_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

EZH1 antibody

Human COLO205; WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate. There is BioGPS gene expression data showing that EZH1 is expressed in COLO205.; EZH1 antibody - N-terminal region (ARP38092_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EZH1 antibody

Immunohistochemical analysis of EZH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EZH1 antibody

Immunohistochemical analysis of EZH1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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