EZH1 antibody

Principal name

EZH1 antibody

Alternative names for EZH1 antibody

ENX-2, KIAA0388, Histone-lysine N-methyltransferase EZH1, Enhancer of zeste homolog 1

SwissProt ID

A7E2Z2 (Bovin), P70351 (Mouse), Q92800 (Human)

Gene ID

2145 (EZH1), 14055 (Ezh1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Can (Canine), Chk (Chicken), Dros (Drosophila), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of EZH1 antibody

In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG) proteins, which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. ENX-2 is another mammalian homolog of the Drosophila gene enhancer of zeste and contains one SET domain. The gene for human ENX-2 maps to chromosome 17q21. ENX-2 expression is ubiquitous in adult and fetal tissue, where it may aid in maintaining heterochromatin stability.

General readings

Abel KJ, Brody LC, Valdes JM, Erdos MR, McKinley DR, Castilla LH, Merajver SD, Couch FJ, Friedman LS, Ostermeyer EA, Lynch ED, King MC, Welcsh PL, Osborne-Lawrence S, Spillman M, Bowcock AM, Collins FS, Weber BL.
Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1.
Genomics. 1996 Oct 15;37(2):161-71.
PMID: 8921387

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EZH1 (N-term) antibody

  Goat Polyclonal Antibody against EZH1  
TA302843 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit polyclonal anti-EZH1 antibody  
Western blot analysis of extracts from HT-29 cells, LOVO cells, A549 cells, NIH-3T3 cells, RAW264.7 cells and COS-7 cells, using EZH1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EZH1 (N-term) antibody

  Rabbit Polyclonal EZH1 Antibody  
Western blot analysis of EZH1 in mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 ug/mL Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit anti-EZH1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using EZH1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit Polyclonal Anti-EZH1 Antibody  
WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysateThere is BioGPS gene expression data showing that EZH1 is expressed in COLO205 Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit Polyclonal Anti-EZH1 Antibody  
WB Suggested Anti-EZH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit Polyclonal EZH1 Antibody  
Western Blot: EZH1 Antibody [TA336341] - 15 ug of HeLa nuclear extract on PVDF membrane. Image provided by verified customer. Rabbit Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

  Rabbit Polyclonal Anti-EZH1 Antibody  
Western blot analysis of EZH1 expression in A549 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EZH1 antibody

Immunohistochemistry (IHC) analyzes of ENX-2 antibody (Cat.-No.: AP06748PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EZH1 (Center) antibody

(LEFT) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. RIGHT) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with EZH1 Antibody (Center) (Cat.#AP12228PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EZH1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using EZH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

EZH1 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using EZH1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

EZH1 - human gene knockout kit via CRISPR

EZH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EZH1 - human gene knockout kit via CRISPR

EZH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EZH1 - human gene knockout kit via CRISPR

EZH1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EZH1 - human gene knockout kit via CRISPR

EZH1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ezh1 - mouse gene knockout kit via CRISPR

Ezh1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ezh1 - mouse gene knockout kit via CRISPR

Ezh1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ezh1 - mouse gene knockout kit via CRISPR

Ezh1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ezh1 - mouse gene knockout kit via CRISPR

Ezh1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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