FAF1 antibody

Principal name

FAF1 antibody

Alternative names for FAF1 antibody

UBXD12, UBXN3A, CGI-03, FAS-associated factor 1,UBX domain-containing protein 3A, UBX domain-containing protein 12

SwissProt ID

P54731 (Mouse), Q924K2 (Rat), Q9UNN5 (Human)

Gene ID

11124 (FAF1), 140657 (Faf1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP)

Background of FAF1 antibody

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis (1). FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain (1,2). Overexpression of FAF1 also decreases the basal level of NF-kappaB activity in transfected 293 cells, inhibits NF-kappaB activity induced by TNF-alpha, IL-1beta and lipopolysaccharide, and prevents NF-kappaB translocation to the nucleus (3), suggesting that another role of FAF1 is to negatively regulate the activity of NF-kappaB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway (4). Multiple differentially spliced isoforms of FAF1 are known to exist.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI10D1 (formerly 10D1)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150 Mouse IgG1 OTI10D1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 (C-term) antibody

  Rabbit Polyclonal FAF1 Antibody  
Western blot analysis of FAF1 in Jurkat cell lysate with FAF1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FAF1 antibody

  Rabbit polyclonal anti-FAF1 antibody  
Western blot analysis of FAF1 in rat kidney tissue extract. Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FAF1 antibody

  Anti-FAF1 Rabbit Polyclonal Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using FAF1 Antibody at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAF1 antibody

  Anti-FAF1 Rabbit Polyclonal Antibody  
Predicted band size: 74 kDa . Positive control: Mouse testis tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAF1 antibody

  Rabbit polyclonal FAF1 antibody  
Western blot analysis of extracts from HepG2 cells and K562 cells, using FAF1 antibody. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI5G9 (formerly 5G9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Mouse IgG1 OTI5G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Mouse IgG2a OTI3E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  Purified FAF1 mouse monoclonal antibody, clone OTI9D12 (formerly 9D12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Mouse IgG2b OTI9D12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI11G10 (formerly 11G10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804144) Mouse IgG2b OTI11G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI10G6 (formerly 10G6)  
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804153) Mouse IgG2b OTI10G6 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  Purified FAF1 mouse monoclonal antibody, clone OTI11H10 (formerly 11H10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Mouse IgG2b OTI11H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAF1 (RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1. Mouse IgG1 OTI10H5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAF1 antibody

  FAF1 mouse monoclonal antibody, clone OTI2F9 (formerly 2F9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FAF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804160) Mouse IgG1 OTI2F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

FAF1 - human gene knockout kit via CRISPR

FAF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FAF1 - human gene knockout kit via CRISPR

FAF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FAF1 - human gene knockout kit via CRISPR

FAF1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FAF1 - human gene knockout kit via CRISPR

FAF1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FAF1

  Recombinant protein of human Fas (TNFRSF6) associated factor 1 (FAF1)  
FAF1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

FAF1 overexpression lysate

FAF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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