FAIM antibody

Principal name

FAIM antibody

Alternative names for FAIM antibody

FAIM1, FAIM-1, Fas Apoptotic Inhibitory Molecule 1

SwissProt ID

Q0IIF6 (Bovin), Q8R5H8 (Rat), Q9NVQ4 (Human), Q9WUD8 (Mouse)

Gene ID

55179 (FAIM), 100362113 (LOC100362113)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), GP (Guinea Pig)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FAIM antibody

FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis. Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types. FAIM is expressed in germil center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism. FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression. Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes. An altertively spliced form of FAIM, termed FAIM-L, is found predomintly in the brain. In the nervous system, the origilly identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-?B pathways. In contrast, FAIM-L does inhibit neurol cell death triggered by death receptors.

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Li, G., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (PT 8), 935-937 (2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Kaku, H., et al. J. Immunol. 183(3):1667-1674(2009)
Segura, M.F., et al. J. Neurosci. 27(42):11228-11241(2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FAIM (Long form) (3-18) antibody

Immunofluorescence localization (arrows) of FAIML proteins in cortical layer V at P5 (3). After blocking, sections were incubated overnight with BCN 4777 (1:600), and was visualized using secondary Alexa Fluorconjugated antibodies. Sections were counterstained with Bisbenzimide, mounted onto slides, and viewed under confocal microscopy. Rabbit Serum Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €250.00
  Antibody BCN

FAIM antibody

TA303191 (0.5µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FAIM antibody

Western blot analysis of FAIM in human spleen tissue lysate with FAIM antibody at (A) 5 and (B) 10 µg/ml. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FAIM antibody

Predicted band size: 20 kDa . Positive control: 293 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAIM antibody

WB Suggested Anti-FAIM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FAIM antibody

WB Suggested Anti-FAIM Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: OVCAR-3 cell lysate. FAIM is supported by BioGPS gene expression data to be expressed in OVCAR3 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

FAIM (121-132) antibody

AP08817PU-N staining FAIM in paraffin-embedded Human Placenta by Immunohistochemistry. Goat IgG Aff - Purified Bear, Bt, Can, Chk, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FAIM Control Peptide

FAIM
50 µg / €160.00
  Acris Antibodies GmbH

FAIM (Center) antibody

Western blot analysis of FAIM Antibody (Center) Cat.-No AP51510PU-N in HL-60 cell line lysates (35ug/lane). This demonstrates the FAIM antibody detected the FAIM protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FAIM antibody

FAIM polyclonal antibody ( Cat # PAB7205 ) ( 0.5 µg/ml ) staining of human lymph node lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

FAIM (Short form) antibody

The Western Blot signal is reduced in extracts from cells with FAIMS gene silencing driven by shRNA interference. PC12 cells infected with a lentivirus carrying a vector for FAIMS shRNAi. Rabbit Serum Hu, Ms, Rt WB
0.1 ml / €300.00
  Antibody BCN

FAIM antibody

Human Jurkat; WB Suggested Anti-FAIM Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; FAIM antibody - N-terminal region (ARP56237_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

FAIM antibody

Human OVCAR-3; WB Suggested Anti-FAIM Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: OVCAR-3 cell lysate. FAIM is supported by BioGPS gene expression data to be expressed in OVCAR3.; FAIM antibody - middle region (ARP56238_P050) in Human OVCAR-3 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FAIM antibody

Immunohistochemistry:FAIM1 Antibody [NB100-77331]-(2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FAIM antibody

Western Blot: FAIM1 Antibody [NBP1-32987] - Western blot analysis of FAIM1 on 293T lysates using NBP1-32987. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

FAIM antibody

Western Blot: FAIM1 Antibody [NBP1-55209] - This Anti-FAIM antibody was used in Western Blot of Jurkat     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FAIM (transcript variant 3)

FAIM Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FAIM (1-213, His-tag)

FAIM Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

FAIM (1-213, His-tag)

FAIM Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

FAIM

FAIM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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