FAK1 / PTK2 antibody

Principal name

FAK1 / PTK2 antibody

Alternative names for FAK1 / PTK2 antibody

FAK, Focal adhesion kinase 1, FADK1, pp125FAK, Protein-tyrosine kinase 2

SwissProt ID

O35346 (Rat), P34152 (Mouse), Q00944 (Chick), Q05397 (Human), Q91738 (Xenla)

Gene ID

5747 (PTK2), 14083 (Ptk2), 2185 (PTK2B)

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Amph (Amphibian), Frog, Rt (Rat), Can (Canine), Xen (Xenopus), Rb (Rabbit), Ze (Zebrafish), Mky (Monkey), Bt (Bat), Bov (Bovine), Eq (Equine)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), ELISA (detection) (E(detection))

Background of FAK1 / PTK2 antibody

Non-receptor protein-tyrosine kise that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neurol cell migration, axon branching and sypse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin sigl transduction, but also in sigling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit sigling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additiol tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous sigling pathways. Promotes activation of phosphatidylinositol 3-kise and the AKT1 sigling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kise sigling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Sigling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitition and proteasomal degradation. Phosphorylates SRC; this increases SRC kise activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kise that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kise domain and inhibits PTK2/FAK1 phosphorylation and sigling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

General readings

1. Cao, Y., et al. (2005) Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase. Biochem. Biophys. Res. Com. 328:560 - 566.
2. Dahmani, S., et al. (2004) Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices. Eur. J. Pharmacol. 489:55 - 58.
3. Dunty, J., et al. (2004) FERM domain interaction promotes FAK signaling. Mol. Cell. Biol. 24(12):5353 - 5368.
4. Ilic, D., et al. (2004) FAK promotes organization of fibronectin matrix and fibrillar adhesions. J. Cell Sci. 117:177 - 187.
5. Lim, Y., et al. (2004) Phosphorylation of focal adhesion kinase at tyrosine 861 is crucial for Ras transformation of fibroblasts. J. Biol. Chem. 279(28):29060 - 29065.
6. Lunn, J.A. and E. Rozengurt (2004) Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. J. Biol. Chem. 279 (43):45266 - 45278.
7. Romanova, L., et al. (2004) Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive. J. Cell Sci. 117:3759 - 3768.
8. Hanks, S.K. and T.R. Polte (1997) Signaling through focal adhesion kinase. BioEssays 19(2):137 - 145.
Ilic, D., C.H. Damsky (1997) Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110:401 - 407.
9. Otey, C.A. (1996) pp125FAK in focal adhesion. Internat. Rev. Cytol. 167:161 - 183.
10. Schlaepfer, D.D. and T. Hunter (1996) Signal transduction from the extracellular matrix—a role for the focal adhesion protein-tyrosine kinase FAK. Cell Struct. Funct. 21(5):445 - 450.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FAK1 / PTK2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506161) Mouse IgG1 OTI4A8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAK1 / PTK2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506174) Mouse IgG1 OTI4D11 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

FAK1 / PTK2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506182) Mouse IgG1 OTI4G7 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FAK1 / PTK2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and PLCG1. Mahlavu cells were stained with anti-PTK2 rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FAK1 / PTK2 antibody

Western blot analysis of extracts from HUVEC cells and MCF-7 cells, using FAK (Ab-843) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK1 / PTK2 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using FAK antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK1 / PTK2 antibody

Western blot analysis of extracts from A549 cells, using FAK (Ab-722) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK1 / PTK2 antibody

Western blot analysis of FAK expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK1 / PTK2 antibody

Western blot analysis of lysate from HUVEC cell line,using PTK2 Antibody (N-term)(Cat. #TA328127).TA328127 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Mouse IgG1 1297CT261.163.145 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FAK1 / PTK2 (Kinase Dom.) antibody

Anti-FAK antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Bt, Can, Chk, Elephant, Fi, Hst, Hu, L, Mky, Ms, Platypus, Prim, Rb, Rt, Tr, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

FAK1 / PTK2 (C-term) antibody

Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

FAK1 / PTK2 (Internal) antibody

Anti-FAK antibody LS-A3392 IHC of human ductal and lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

FAK1 / PTK2 pTyr397 (incl. pos. control) antibody

Detection of endogenous FAK: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab AM00059PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Lane 1: HeLa 
Lane 2: HepG2 
Lane 3: HEK293 
Lane 4: SH-SY5Y 
Lane 5: MDCK 
Lane 6: PC12 
Lane 7: CMT 93 
Lane 8: Neuro 2A
Lane 9: 3T3 Mouse IgG1 2D11 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

FAK1 / PTK2 antibody

HeLa cells (lane 1), mouse L929 cells (lane 2), and rat PC12 cells (lane 3) were resolved by SDS-PAGE and transferred to PVDF. The membranes were incubated with this FAK monoclonal antibody (clone 34Q36) at a concentration of 1 μg/mL for two hours at room temperature. After washing, the membranes were incubated with a goat F(ab’)2 anti-mouse IgG alkaline phosphatase conjugated antibody at a 1:2000 dilution. Bands were detected with CDP-substrate using the WesternStarTM method (Tropix) and Kodak BioMax film. Mouse IgG2b 34Q36 Purified Hu, Ms, Rt WB
0.1 mg / €540.00
  Acris Antibodies GmbH

FAK1 / PTK2 (1-423) antibody

Tonsil: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG1 4.47 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FAK1 / PTK2 antibody

Western blot analysis using FAK antibody Cat.-No AM06120SU-N against truncated FAK recombinant protein. Mouse IgG 4A9D6 Ascites Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

FAK1 / PTK2 antibody

Western blot analysis using FAK mAb against FAK(AA: 354-533)-hIgGFc transfected HEK293 cell lysate. Mouse IgG1 10H7A6 Ascites Hu E, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

FAK1 / PTK2 antibody

Immunofluorescence analysis of A549 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 10H7E9 Ascites Hu E, F, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

FAK1 / PTK2 antibody

Immunohistochemical analysis of paraffin-embedded cervices tumour using FAK antibody Cat.-No AM06508SU-N with DAB staining Mouse IgG1 10H7 Ascites Hu, Ms E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

FAK1 / PTK2 (706-1052) antibody

FAK1 / PTK2 Mouse IgG1 12G4 Azide Free Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

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