FAK2 / PTK2B antibody

Principal name

FAK2 / PTK2B antibody

Alternative names for FAK2 / PTK2B antibody

PYK2, RAFTK, Protein tyrosine kinase 2 beta, Focal adhesion kinase 2, CAK-beta, CADTK, Proline-rich tyrosine kinase 2, Cell adhesion kinase beta, Calcium-dependent tyrosine kinase, Related adhesion focal tyrosine kinase

SwissProt ID

P70600 (Rat), Q14289 (Human), Q9QVP9 (Mouse)

Gene ID

2185 (PTK2B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C)

Background of FAK2 / PTK2B antibody

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels.

General readings

1. Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.; "Identification of a new Pyk2 target protein with Arf-GAP activity."; Mol. Cell. Biol. 19:2338-2350(1999).
2. Schlessinger J., 1995. Protein tyrosine kinase PYK2 involved in calcium-induced regulation of ion channel and MAP kinase functions. Nature376:737-745
3. Candice K. Klingbeil, Christof R. Hauck, Datsun A. Hsia, K.C. Jones, Shannon R. Reider and David D. Schlaepferaaaaaa
Targeting Pyk2 to ß1-Integrin–containing Focal Contacts Rescues Fibronectin-stimulated Signaling and Haptotactic Motility Defects of Focal Adhesion Kinase–null Cells
Journal of Cell Biology 152:97-110 (2001)
4. Avraham H., Park S.Y., Schinkmann K., Avraham S., 2000. RAFTK/Pyk2-mediated cellular signalling. Cell. Signal12:123-133

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FAK2 / PTK2B antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2B and YES1 Huh7 cells were stained with anti-PTK2B rabbit purified polyclonal 1:1200 and anti-YES1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FAK2 / PTK2B antibody

Western blot analysis of extracts from 3T3 cells, using PYK2 (Ab-579) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK2 / PTK2B antibody

Predicted band size: 116 kDa . Positive control: 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells lysate Lane2: Treated with the peptide Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAK2 / PTK2B pTyr402 antibody

Transactivation of PYK2 Whole cell lysates of serum starved HepG2 tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab PYK2-14F6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1:Co; lane 2: serum; lane 3: EGF; lane 4: H2O2;lane 5: Anisomycin; lane 6: Sorbit; lane 7: Arsen; lane 8:Ceramide. Mouse IgG1 14F6 Purified Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using PYK2 antibody Cat.-No AM06233SU-N Mouse IgG2a 5E2D5 Ascites Hu E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

FAK2 / PTK2B pTyr402 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 pTyr402 antibody AP02475PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

FAK2 / PTK2B pTyr402 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 pTyr402 antibody AP02475PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 antibody AP02719PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Pyk2 antibody AP02719PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Immunohistochemistry (IHC) analyzes of PYK2 antibody (Cat.-No.: AP06298PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Immunohistochemistry (IHC) analyzes of PYK2 antibody (Cat.-No.: AP06408PU-N) in paraffin-embedded human brain colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FAK2 / PTK2B (N-term) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µl / €470.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

Human Colon, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

FAK2 / PTK2B pTyr579 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody AP55794PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

FAK2 / PTK2B pTyr579 antibody

Immunohistochemical analysis of paraffin-embedded human brain tissue using PYK2 (Phospho-Tyr579) antibody AP55794PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €270.00
  Acris Antibodies GmbH

FAK2 / PTK2B antibody

PTK2B MaxPab polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

FAK2 / PTK2B antibody

PTK2B MaxPab polyclonal antibody. Western Blot analysis of PTK2B expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

FAK2 / PTK2B antibody

PTK2B monoclonal antibody (M01), clone 1F9 Western Blot analysis of PTK2B expression in K-562 ( Cat # L009V1 ). Mouse IgG2b 1F9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FAK2 / PTK2B antibody

FAK2 / PTK2B Mouse IgG2a X1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FAK2 / PTK2B antibody

FAK2 / PTK2B Mouse IgG2a 3G14 Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

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