FAM119A antibody
Principal name
FAM119A antibody
Alternative names for FAM119A antibody
HCA557B, Hepatocellular carcinoma-associated antigen 557b
Ncbi ID
9606, NP_660323, NP_001120867.1, NM_145280, NM_001127395, NP_001120867, NP_080240.1, NM_025964
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of FAM119A antibody
- Kit Component:
- - KN310000G1, 2310038H17Rik gRNA vector 1 in pCas-Guide vector
- KN310000G2, 2310038H17Rik gRNA vector 2 in pCas-Guide vector
- KN310000D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kagaya A, Shimada H, et al. (2011). Proteome Sci. 9(1):31.
Human Liver; WB Suggested Anti-FAM119A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; FAM119A antibody - middle region (ARP52844_P050) in Human Liver cells using Western Blot
Human Brain; WB Suggested Anti-FAM119A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain; FAM119A antibody - N-terminal region (ARP52843_P050) in Human Brain cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM119A (RC207629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM119A.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FAM119A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503704)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FAM119A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503704)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM119A (RC207629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM119A.
HEK293T cells transfected with either RC207629 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FAM119A antibody(TA503704), and then analyzed by flow cytometry.
Anti-FAM119A mouse monoclonal antibody (TA503803) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FAM119A(RC207629).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM119A (RC207629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM119A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM119A (RC207629, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM119A.
WB Suggested Anti-METTL21A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human brain
WB Suggested Anti-METTL21A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Liver
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503704 | FAM119A antibody |
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| FAM119A mouse monoclonal antibody, clone OTI2G7 (formerly 2G7) | |||||||
 |
Mouse | IgG2b | OTI2G7 | Purified | Hu | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA340404 | FAM119A antibody |
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| Rabbit Polyclonal Anti-METTL21A Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA340405 | FAM119A antibody |
||||||
| Rabbit Polyclonal Anti-METTL21A Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA503703 | FAM119A antibody |
||||||
| FAM119A mouse monoclonal antibody, clone OTI2D6 (formerly 2D6) | |||||||
 |
Mouse | IgG1 | OTI2D6 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA503803 | FAM119A antibody |
||||||
| FAM119A mouse monoclonal antibody, clone OTI2D3 (formerly 2D3) | |||||||
 |
Mouse | IgG2b | OTI2D3 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA503923 | FAM119A antibody |
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| FAM119A mouse monoclonal antibody, clone OTI1C8 (formerly 1C8) | |||||||
 |
Mouse | IgG2a | OTI1C8 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN207629 | METTL21A - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN207629BN | METTL21A - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN207629LP | METTL21A - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN207629RB | METTL21A - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310000 | Mettl21a - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310000BN | Mettl21a - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310000LP | Mettl21a - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN310000RB | Mettl21a - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307629 | FAM119A (transcript variant 1) |
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| Recombinant protein of human family with sequence similarity 119, member A (FAM119A), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
|
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| TP760710 | FAM119A (full length, N-term HIS tag, transcript variant 2) |
||||
| Purified recombinant protein of Human methyltransferase like 21A (METTL21A), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€199.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50987PU-N | FAM119A (93-218, His-tag) |
||||
 |
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.1 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50987PU-S | FAM119A (93-218, His-tag) |
||||
|
Human | Purified | > 85 % by SDS - PAGE | E. coli |
20 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY408004 | METTL21A overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY426777 | METTL21A overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
