FANCC antibody

Principal name

FANCC antibody

Alternative names for FANCC antibody

Fanconi anemia group C protein, FAC, FACC

SwissProt ID

O19104 (Bovin), O35870 (Rat), P50652 (Mouse), Q00597 (Human)

Gene ID

2176 (FANCC), 6217652 (atpA)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Chimp (Chimpanzee), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of FANCC antibody

Fanconi anemia (FA) is an autosomal recessive disorder characterized by bone marrow failure, birth defects and chromsomal instability (1,2). The FA Group C complementation group gene encodes the protein FANCC, which is located in both cytoplasmic and nuclear compartments. FANCC is expressed in a cell cycle-dependent manner, with the lowest levels at the G1/S boundary and the highest levels in the M-phase. The FANCC protein interacts with other FA complementation group proteins as well as non-FA proteins (3). A human a spectrin II (designated aSpIIs) acts as a scaffold to enhance interactions between FANCC and FANCA to form a nuclear complex (4,5). Another binding partner of FANCC is the BTB/POZ domain containing protein FAZF, which is a transcriptional repressor (6). In hematopoietic cells expressing mutant FANCC, PKR is constitutively phosphorylated and has increased binding affinity for double-stranded RNA (7,8), which suggests that FANCC indirectly suppresses the activity of PKR. These cells are also apoptotic and are hypersensitive to IFNg and TNFa (8). In addition, FANCC protein is involved in the activation of STAT1 through receptors for at least three hematopoietic growth and survival factors (8).

General readings

1. Hirano,S., Yamamoto,K., Ishiai,M., Yamazoe,M., Arakawa,H., Buerstedde,J.M., Enomoto,T., Takeda,S., Thompson,L.H. and Takata,M. (2005)
Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM. EMBO J. 24 (2), 418-427.
2. Mi,J. and Kupfer,G.M. (2005) The Fanconi anemia core complex associates with chromatin during S phase. Blood 105 (2), 759-766.
3. Zhang,X., Li,J., Sejas,D.P., Rathbun,K.R., Bagby,G.C. and Pang,Q. (2004) The Fanconi anemia proteins functionally interact with the protein
kinase regulated by RNA (PKR). J. Biol. Chem. 279 (42), 43910-43919.
4. Niedzwiedz,W., Mosedale,G., Johnson,M., Ong,C.Y., Pace,P. and Patel,K.J. (2004) The Fanconi anaemia gene FANCC promotes
homologous recombination and error-prone DNA repair. Mol. Cell 15 (4), 607-620.
5. Zanier,R., Briot,D., Dugas du Villard,J.A., Sarasin,A. and Rosselli,F. (2004) Fanconi anemia C gene product regulates expression of genes
involved in differentiation and inflammation. Oncogene 23 (29), 5004-5013.
6. Brodeur,I., Goulet,I., Tremblay,C.S., Charbonneau,C., Delisle,M.C., Godin,C., Huard,C., Khandjian,E.W., Buchwald,M., Levesque,G. and
Carreau,M. (2004) Regulation of the Fanconi anemia group C protein through proteolytic modification. J. Biol. Chem. 279 (6), 4713-4720.
7. Strathdee,C.A., Duncan,A.M. and Buchwald,M. (1992) Evidence for at least four Fanconi anaemia genes including FACC on chromosome 9.
Nat. Genet. 1 (3), 196-198.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCC antibody

Sample (30 ug of whole cell lysate). . A: NIH-3T3. . 7.5% SDS PAGE. . FANCC antibody. . TA308740 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC (1-275) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

FANCC antibody

Detection of FANC C in transfected COS1 cell lysate Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Western Blot: FANCC Antibody (8F3) - Western blot on hydroxyurea (HU) synchronized 293 FANCC E2 cells. Mouse IgG2b 8F3 Purified Hu ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC (96-112) antibody

WB using Anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate. The identity of the lower molecular weight band is unknown. The primary antibody was diluted to 1:1,500. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] was used at 1:10,000. Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Predicted band size: 63 kDa . Positive control: Brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC (C-term) antibody

Western blot analysis of lysates from HepG2,HL-60 cell line (from left to right),using FANCC Antibody (C-term)(Cat. #TA325105).TA325105 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Western blot analysis of extracts of brain cell lines, using FANCC antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Western Blot: FANCC Antibody [TA336732] - Western Blot on cells overexpressing FANCC probed with 1. pre-immune serum and 2. Rabbit Purified Hu ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Western Blot: FANCC Antibody [TA336733] - Western blot analysis of FANCC expression in 1) NTera2 and 2) Cos 7 whole cell lysates. Rabbit Purified Hu, Prim F, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCC antibody

Immunohistochemical staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

FANCC (96-112) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE)on Brain, cortex. Rabbit Aff - Purified Hu, Mky E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FANCC (C-term) antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

FANCC antibody

FANCC Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FANCC antibody

Western Blot analysis of FANCC expression in transfected 293T cell line (H00002176-T01) by FANCC MaxPab polyclonal antibody.

Lane 1: FANCC transfected lysate(61.38 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

FANCC antibody

Western Blot analysis of FANCC expression in transfected 293T cell line (H00002176-T01) by FANCC MaxPab polyclonal antibody.

Lane 1: FANCC transfected lysate(61.38 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

FANCC antibody

FANCC Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

FANCC antibody

FANCC MaxPab rabbit polyclonal antibody. Western Blot analysis of FANCC expression in human kidney. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FANCC antibody

Western Blot analysis of FANCC expression in transfected 293T cell line by FANCC monoclonal antibody (M01), clone 6E7.

Lane 1: FANCC transfected lysate(63.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 6E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FANCC antibody

Western blot using FANCC polyclonal antibody ( Cat # PAB10046 ) shows detection of a band at ~63 kDa ( arrowhead ) corresponding to FANCC present in a HeLa whole cell lysate.
The identity of the lower molecular weight band is unknown.
Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to1:1,500 in PBS supplemented with 1% normal goatserum and 0.1% BLOTTO.
The membrane was washed and reacted with a 1:10,000 dilution ofIRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).
Molecular weight estimation was made bycomparison to prestained MW markers ( indicated at left ).
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

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