FANCF antibody

Principal name

FANCF antibody

Alternative names for FANCF antibody

Fanconi anemia group F protein, FACF

SwissProt ID

Q9NPI8 (Human)

Gene ID

2188 (FANCF)

Available reactivities

Hu (Human), Mky (Monkey), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FANCF antibody

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FANCF (217-228) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with AP22410PU-N 1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AP22410PU-N (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

FANCF (C-term) antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with TA311512 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1:1000) in Lane C. Mock-transfected HEK293 probed with TA311512 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FANCF antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/50-100  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using FANCF antibody at dilution 1/100, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FANCF (Internal) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Mky E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FANCF antibody

Western blot using anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

FANCF antibody

Western Blot analysis of FANCF expression in transfected 293T cell line (H00002188-T01) by FANCF MaxPab polyclonal antibody.

Lane 1: FANCF transfected lysate(41.14 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

FANCF antibody

FANCF Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

FANCF antibody

FANCF Mouse IgG1 3C4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

FANCF antibody

Western blot using FANCF polyclonal antibody ( Cat # PAB10011 ) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA ( lanes 3 and 4 ).
This band ( indicated by arrowhead ) is approximately 42.3 kDa in size.
The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein ( lanes 1 and 2 ).
Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.
No apparent difference was noted upon irradiation.
The strong band at ~60kDa appears to be non-specific.
Personal communication, N. Howlett, University of Rhode Island, Kingston, RI. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

FANCF antibody

FANCF Rabbit Hu E, WB
50 µg / €440.00
  Novus Biologicals Inc.

FANCF antibody

FANCF Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FANCF

FANCF Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

FANCF

FANCF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

FANCF

FANCF Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

FANCF 293T Cell Transient Overexpression Lysate(Denatured)

FANCF 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

15 Item(s)

per page
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