FAST kinase antibody

Principal name

FAST kinase antibody

Alternative names for FAST kinase antibody

FASTK, Fas-activated serine/threonine kinase

SwissProt ID

Q14296 (Human), Q9JIX9 (Mouse)

Gene ID

10922 (FASTK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FAST kinase antibody

The Human homolog FAST-1 and the corresponding mouse homolog, designated FAST-2, share significant sequence homology with xFAST-1, including a conserved N-terminal forkhead domain that consists of 110 amino acid residues and is essential for binding DNA and regulating transcription in embryogenesis, in tumorigenesis and in the maintenance of differentiated cell states. FAST-1 and FAST-2 also contain a distinct C-terminal Smad interaction domain that is required for the association with various Smad proteins, including Smad2, Smad3 and Smad4. Expression of FAST-1 and FAST-2 is predominantly observed during early development, with lower levels detected in adult tissues. FAST-1 and FAST-2 mediated DNA binding is attenuated by both TFGβ and activin, indicating that these FAST proteins mediate TFGβ induced signal transduction.

General readings

Simarro, M., et al. J. Immunol. 184(9):5325-5332(2010)
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Simarro, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(27):11370-11375(2007)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Izquierdo, J.M., et al. J. Biol. Chem. 282(3):1539-1543(2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FAST kinase antibody

FAST antibody detects FASTK protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lys Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FAST kinase (C-term) antibody

The anti-FASTK Pab is used in Western blot to detect FASTK in HL-60 cell lysate. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FAST kinase antibody

Western blot (WB) analysis of FAST antibody (Cat.-No.: AP20588PU-N) in extracts from pc12 cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FAST kinase (C-term) antibody

Formalin fixed and paraffin embedded human liver tissue reacted with FAST kinase (C-term) Antibody (C-term) Cat.-No followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FAST kinase antibody

FAST kinase Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FAST kinase antibody

FAST kinase Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FAST kinase antibody

Western Blot analysis of FASTK expression in transfected 293T cell line by FASTK monoclonal antibody (M02), clone 2B7.

Lane 1: FASTK transfected lysate(61.104 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FAST kinase antibody

The FASTK polyclonal antibody ( Cat # PAB4642 ) is used in Western blot to detect FASTK in HL-60 cell lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

FAST kinase antibody

Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Por, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FAST kinase antibody

Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Por, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FAST kinase antibody

Immunofluorescent analysis of FAST Kinase staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Por, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

FAST kinase antibody

Immunofluorescence: FASTK Antibody [NBP1-31323] - Immunofluorescence analysis of HeLa, using FASTK antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

FAST kinase antibody

Immunohistochemistry-Paraffin: FASTK Antibody [NBP1-84729] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FAST kinase (transcript variant 4)

FAST kinase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FAST kinase

FAST kinase Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

FAST kinase

FAST kinase Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FAST kinase

FAST kinase Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

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