Fatty acid alpha-hydroxylase antibody

Principal name

Fatty acid alpha-hydroxylase antibody

Alternative names for Fatty acid alpha-hydroxylase antibody

FA2H, FAAH, Fatty acid 2-hydroxylase

SwissProt ID

Q2LAM0 (Rat), Q5MPP0 (Mouse), Q7L5A8 (Human)

Gene ID

79152 (FA2H), 338521 (Fa2h), 307855

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Fatty acid alpha-hydroxylase antibody

2-Hydroxysphingolipids are a subset of sphingolipids containing 2-hydroxy fatty acids. The 2-hydroxylation occurs during de novo ceramide synthesis and is catalyzed by fatty acid 2-hydroxylase (also known as fatty acid alpha-hydroxylase). In mammals, 2-hydroxysphingolipids are present abundantly in brain because the major myelin lipids galactosylceramides and sulfatides contain 2-hydroxy fatty acids. Here we report identification and characterization of a human gene that encodes a fatty acid 2-hydroxylase. Data base searches revealed a human homologue of the yeast ceramide 2-hydroxylase gene (FAH1), which we named FA2H. The FA2H gene encodes a 372 - amino acid protein with 36% identity and 46% similarity to yeast Fah1p. The amino acid sequence indicates that FA2H protein contains an N-terminal cytochrome b5 domain and four potential transmembrane domains. FA2H also contains the iron-binding histidine motif conserved among membrane-bound desaturases/hydroxylases. COS7 cells expressing human FA2H contained 3-20-fold higher levels of 2-hydroxyceramides (C16, C18, C24, and C24:1) and 2-hydroxy fatty acids compared with control cells. Microsomal fractions prepared from transfected COS7 cells showed tetracosanoic acid 2-hydroxylase activities in an NADPH- and NADPH: cytochrome P-450 reductase-dependent manner. FA2H lacking the N-terminal cytochrome b5 domain had little activity.

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fatty acid alpha-hydroxylase antibody

  Rabbit Polyclonal Anti-Fa2h Antibody  
Fatty acid alpha-hydroxylase Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fatty acid alpha-hydroxylase antibody

  FA2H mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)  
Immunofluorescent staining of COS7 cells transiently transfected with pCMV6-ENTRY FA2H(RC202093)by using Anti-FA2H mouse monoclonal antibody (TA506817) at 1:100 dilutions. Mouse IgG1 OTI2E3 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Fatty acid alpha-hydroxylase antibody

  FA2H mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)  
Immunofluorescent staining of COS7 cells transiently transfected with pCMV6-ENTRY FA2H(RC202093) by using Anti-FA2H mouse monoclonal antibody (TA506818) at 1:100 dilutions. Mouse IgG2b OTI1C5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Fatty acid alpha-hydroxylase antibody

  FA2H mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)  
Immunofluorescent staining of COS7 cells, transiently transfected with pCMV6-ENTRY FA2H(RC202093) by using Anti-FA2H mouse monoclonal antibody (TA506832) at 1:100 dilution. Mouse IgG2b OTI1B5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Fatty acid alpha-hydroxylase antibody

Fatty acid alpha-hydroxylase Rabbit Purified Hu WB
0.1 mg / €490.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Fa2h - mouse gene knockout kit via CRISPR

Fa2h - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Fa2h - mouse gene knockout kit via CRISPR

Fa2h - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fa2h - mouse gene knockout kit via CRISPR

Fa2h - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fa2h - mouse gene knockout kit via CRISPR

Fa2h - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Fatty acid alpha-hydroxylase

  Recombinant protein of human fatty acid 2-hydroxylase (FA2H)  
Fatty acid alpha-hydroxylase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

FA2H overexpression lysate

FA2H overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, FA2H (mGFP-tagged) - Human fatty acid 2-hydroxylase (FA2H), 200 uL, >10^7 TU/mL

Lenti ORF particles, FA2H (mGFP-tagged) - Human fatty acid 2-hydroxylase (FA2H), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, FA2H (Myc-DDK tagged) - Human fatty acid 2-hydroxylase (FA2H) , 200 uL, >10^7 TU/mL

Lenti ORF particles, FA2H (Myc-DDK tagged) - Human fatty acid 2-hydroxylase (FA2H) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Fa2h (GFP-tagged) - Mouse fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fa2h (GFP-tagged) - Mouse fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Fa2h (Myc-DDK-tagged) - Mouse fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fa2h (Myc-DDK-tagged) - Mouse fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Fa2h (GFP-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fa2h (GFP-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Fa2h (Myc-DDK-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fa2h (Myc-DDK-tagged ORF) - Rat fatty acid 2-hydroxylase (Fa2h), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

17 Item(s)

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