FBL antibody

Principal name

FBL antibody

Alternative names for FBL antibody

FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1

SwissProt ID

P15646 (Yeast), P22087 (Human), P22232 (Xenla), P22509 (Rat), P35550 (Mouse), Q9W1V3 (Drome)

Gene ID

2091 (FBL), 851548 (NOP1), 14113 (Fbl), 292747

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Pl (Plant), Ye (Yeast), Ce (C. elegans), Dros (Drosophila), Por (Porcine), Chk (Chicken), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of FBL antibody

Fibrillarin / Nop1p, was origilly identified as a nucleolar protein of baker's yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is Fibrillarin (accession P22087), a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human Fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N termil 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the Fibrillarin domain. A Fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-termil extension but is clearly homologous to the other sequences throughout the entire Fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be Fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

General readings

Amin MA, et al. (2007) Biochem Biophys Res Commun. 360(2):320-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBL antibody

  FBL Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using FBL antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

  Mouse Monoclonal Fibrillarin Antibody (38F3)  
Western Blot: Fibrillarin Antibody (38F3) [TA336627] - Western blot analysis of Fibrillarin expression on yeast protein extracts using TA336627. Mouse IgG1 38F3 Purified Ce, Chk, Dros, Hu, Ms, Rt C, F, ICC/IF, P, WB
0.25 ml / €325.00
  OriGene Technologies, Inc.

FBL antibody


FBL antibody Cat.-No AP32019PU-N staining of HEK293 lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FBL antibody

  Goat Anti-Fibrillarin / FBL Antibody  
(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

  Anti-FBL Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: 293, Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: 293 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FBL antibody

  Rabbit Polyclonal Anti-FBL Antibody  
WB Suggested Anti-FBL Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 12500; Positive Control: Jurkat cell lysate; FBL is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

Figure 2. Human SH-SY5Y cells stained with mouse-anti-Fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Mouse IgG1 38F3 Ig Fraction Ce, Dros, Hu, Pl, Rt, S. pombe C, ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

FBL (Center) antibody

Western blot analysis of anti-FBL Antibody (Center) (Cat.#AP12421PU-N) in K562 cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FBL (Nucleolar Marker) antibody

Human SH-SY5Y cells stained with Fibrillarin/Nop1p  showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Cells are also stained with Neurofilament NF-H. Mouse IgG1 Purified Ce, Dros, Hu, Ms ICC/IF, WB
0.5 ml / €310.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

FBL - human gene knockout kit via CRISPR

FBL - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FBL - human gene knockout kit via CRISPR

FBL - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FBL - human gene knockout kit via CRISPR

FBL - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FBL - human gene knockout kit via CRISPR

FBL - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fbl - mouse gene knockout kit via CRISPR

Fbl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Fbl - mouse gene knockout kit via CRISPR

Fbl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fbl - mouse gene knockout kit via CRISPR

Fbl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fbl - mouse gene knockout kit via CRISPR

Fbl - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBL

  Recombinant protein of human fibrillarin (FBL)  
FBL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FBL (83-321, His-tag)

FBL Human Purified > 90 % by SDS – PAGE E. coli
50 µg / €750.00
  Acris Antibodies GmbH

FBL (83-321, His-tag)

FBL Human Purified > 90 % by SDS – PAGE E. coli
10 µg / €300.00
  Acris Antibodies GmbH

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