FBL antibody

Principal name

FBL antibody

Alternative names for FBL antibody

FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1

SwissProt ID

P15646 (Yeast), P22087 (Human), P22232 (Xenla), P22509 (Rat), P35550 (Mouse), Q9W1V3 (Drome)

Gene ID

2091 (FBL), 851548 (NOP1)

Available reactivities

Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Hu (Human), Ms (Mouse), Pl (Plant), Rt (Rat), Ye (Yeast), Bov (Bovine), Can (Canine), Xen (Xenopus), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of FBL antibody

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

General readings

Amin MA, et al. (2007) Biochem Biophys Res Commun. 360(2):320-6.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBL antibody

Western blot analysis of extracts of various cell lines, using FBL antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

Western Blot: Fibrillarin Antibody (38F3) [TA336627] - Western blot analysis of Fibrillarin expression on yeast protein extracts using TA336627. Mouse IgG1 38F3 Purified Ce, Chk, Dros, Hu, Ms, Rt C, F, ICC/IF, P, WB
0.25 ml / €325.00
  OriGene Technologies, Inc.

FBL antibody


FBL antibody Cat.-No AP32019PU-N staining of HEK293 lysate at 0.1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

FBL antibody

(1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

Predicted band size: 34 kDa . Positive control: 293, Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2: 293 and Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FBL antibody

WB Suggested Anti-FBL Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 12500; Positive Control: Jurkat cell lysate; FBL is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBL antibody

Figure 2. Human SH-SY5Y cells stained with mouse-anti-Fibrillarin, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Mouse IgG1 38F3 Ig Fraction Ce, Dros, Hu, Pl, Rt, S. pombe C, ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

FBL antibody

FBL Mouse IgG3 28F2 Ye ICC/IF, IP, WB
0.2 ml / €340.00
  Acris Antibodies GmbH

FBL (Center) antibody

Western blot analysis of anti-FBL Antibody (Center) (Cat.#AP12421PU-N) in K562 cell line lysates (35ug/lane). FBL (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FBL antibody

FBL Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FBL antibody

Detection limit for recombinant GST tagged FBL is approximately 3ng/ml as a capture antibody. Mouse IgG2a 4G9-E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FBL antibody

Detection limit for recombinant GST tagged FBL is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 3E10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

FBL antibody

Western Blot analysis of yeast protein extracts stained with FBL monoclonal antibody, clone 38F3 ( Cat # MAB2309 ). A single band at ~34kDa. Mouse IgG1 38F3 Supernatant Hu, Pl, Rt, Ye ICC/IF, P, WB
0.25 ml / €560.00
  Abnova Taiwan Corp.

FBL antibody

FBL Mouse IgG3 28F2 Ye ICC/IF, IP, WB
0.2 ml / €560.00
  Abnova Taiwan Corp.

FBL antibody

Human Intestine; Rabbit Anti-FBL Antibody. Catalog Number: ARP40366. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; FBL antibody - N-terminal region (ARP40366_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

FBL (Nucleolar Marker) antibody

Human SH-SY5Y cells stained with Fibrillarin/Nop1p  showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue. Cells are also stained with Neurofilament NF-H. Mouse IgG1 Purified Ce, Dros, Hu, Ms ICC/IF, WB
0.5 ml / €310.00
  Neuromics Antibodies

FBL antibody

Western Blot: Fibrillarin Antibody (28F2) [NBP1-05223] - Western Blot analysis of whole yeast protein extracts detect Fibrillarin/Nop1p.  Molecular weight detected is 34.5 kDa. Mouse 28F2 Supernatant Ye ICC/IF, IP, WB
0.5 ml / €340.00
  Novus Biologicals Inc.

FBL antibody

FBL Goat Purified Hu, Ms, Rt, Xen P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

FBL antibody

FBL Rabbit Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBL

FBL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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