FBW1B antibody

Principal name

FBW1B antibody

Alternative names for FBW1B antibody

FBXW11, BTRCP2, FBXW1B, KIAA0696, beta-TrCP2, HOS, F-box/WD repeat-containing protein 11, F-box/WD repeat-containing protein 1B, F-box and WD repeats protein beta-TrCP2, Homologous to Slimb protein

SwissProt ID

Q9UKB1 (Human)

Gene ID

23291 (FBXW11)

Available reactivities

Hu (Human), Bov (Bovine), Chimp (Chimpanzee), Chk (Chicken), Ms (Mouse), Can (Canine), Eq (Equine), Ze (Zebrafish), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of FBW1B antibody

FBXW11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class and, in addition to an F-box, contains multiple WD40 repeats. This gene contains at least 14 exons, and its alternative splicing generates 3 transcript variants diverging at the presence/absence of two alternate exons.

General readings

Peschiaroli, A., et al. Oncogene 29(9):1384-1393(2010)
Lievens, S., et al. J. Proteome Res. 8(2):877-886(2009)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Peschiaroli, A., et al. Mol. Cell 23(3):319-329(2006)
Sabile, A., et al. Mol. Cell. Biol. 26(16):5994-6004(2006).

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBW1B antibody

WB using Anti-ßTrCP2 antibody shows detection of mouse and human ßTrCP2 (arrowhead) in NIH3T3 (lane 1) and 293 (lane 2) whole cell lysates.The primary antibody was used at1:200.  Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking buffer (p/n MB-070) for 1 h at 4 ?. Rabbit IgG Aff - Purified Bov, Can, Chimp, Chk, Eq, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBW1B (Center) antibody

FBXW11 Antibody (Center) (Cat. #TA324389) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the FBXW11 antibody detected the FBXW11 protein (arrow). Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FBW1B antibody

FBW1B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBW1B antibody

WB Suggested Anti-FBXW11 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysateFBXW11 is supported by BioGPS gene expression data to be expressed in HEK293T Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBW1B Control Peptide

FBW1B Purified
50 µg / €190.00
  Acris Antibodies GmbH

FBW1B antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between WEE1 and FBXW11. HeLa cells were stained with anti-WEE1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 2E7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

FBW1B antibody

Western blot using FBXW11 polyclonal antibody ( Cat # PAB10237 ) shows detection of mouse and human FBXW11 ( arrowhead ) in NIH/3T3 ( lane 1 ) and 293 ( lane 2 ) whole cell lysates, respectively.
The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted.
The identity of faint higher molecular weight bands is not known.
The primary antibody was used at a 1 : 200 dilution incubated in 5% BLOTTO overnight at 4°C.
Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1 : 20,000 in blocking buffer for 1 h at 4°C. Bov, Chimp, Chk, Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

FBW1B antibody

Human 721_B; WB Suggested Anti-FBXW11 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. FBXW11 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; FBXW11 antibody - N-terminal region (ARP43129_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

FBW1B antibody

Human 293T; WB Suggested Anti-FBXW11 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. FBXW11 is supported by BioGPS gene expression data to be expressed in HEK293T.; FBXW11 antibody - N-terminal region (ARP43379_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Can, Chk, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

FBW1B antibody

Western  Blot:  FBXW11 Antibody [NBP1-33738] – Western blot analysis of FBXW11 on H1299 lysates using NBP1-33738 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel. Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

FBW1B antibody

Western Blot: FBXW11 Antibody [NBP1-55052] Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

FBW1B antibody

FBW1B Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

FBW1B antibody

FBW1B Rabbit Aff - Purified Hu E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBW1B (transcript variant 2)

FBW1B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FBW1B

FBW1B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FBW1B

FBW1B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

FBXW11 overexpression lysate

FBXW11 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

FBXW11 overexpression lysate

FBXW11 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

FBXW11 overexpression lysate

FBXW11 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

FBXW11 overexpression lysate

FBXW11 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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