FBXO32 antibody

Principal name

FBXO32 antibody

Alternative names for FBXO32 antibody

FBXO32, F-box only protein 32, Muscle atrophy F-box protein, MAFbx, Atrogin-1

SwissProt ID

Q1A730 (Pig), Q2KHT6 (Bovin), Q91Z62 (Rat), Q969P5 (Human), Q9CPU7 (Mouse)

Gene ID

114907 (FBXO32), 67731 (Fbxo32)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of FBXO32 antibody

Atrogin-1 (Atr1), also known as Muscle Atrophy F-box (MAFbx), is a member of SCF family of E3 ubiquitin ligases. It is expressed in heart and skeletal muscle. It is a critical component in the enhanced proteolysis leading to muscle atrophy in diseases such as diabetes, cancer and renal failure (1). Ubiquitin-dependent proteolysis regulates protein abundance and serves as a central regulatory control in many biological processes; it is mediated by the ubiquitin ligases, which represent a diverse family of proteins and protein complexes (2). A broad range of proteins involved in cell cycle progression, signal transduction, transcription, and development are ubiquitinated by the largest family of ubiquitin ligases, the SCF (Skp1, Cdc53/Cullin1, F-box protein) and SCF-like complexes. Among them, the F-box protein serves as the receptor for the target protein. Mice deficient in Atrogin-1/MAFbx were found to be resistant to atrophy, whereas overexpression of Atrogin-1/MAFbx suppressed MyoD-induced differentiation and inhibited myotube formation (3).
MyoD is required for myogenic stem cell function in adult skeletal muscle and it controls the identity and differentiation of myotube (4). Atrogin-1/MAFbx (Atr1) interacts with MyoD via an inverted LXXLL motif located in a series of helical leucine-charged residue-rich domains. Mutation in the LXXLL core motif represses ubiquitination and degradation of MyoD induced by Atrogin-1/MAFbx. This Atrogin-1 and MyoD interface mediates the binding to liganded nuclear receptors and promotes its degradation (5). Hence, up-regulation of Atrogin-1 in proliferating myoblasts antagonizes differentiation inducing MyoD degradation and preventing muscle-specific gene activation. Atrogin-1/MAFbx is approximately a 43kDa protein (355 amino acids).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO32 antibody

TA305697 (0.1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBXO32 antibody

Western blot analysis of Fbx32 using rat kidney tissue lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBXO32 antibody

Predicted band size: 25 kDa . Positive control: HT-29 and K562 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: K562 cell lysate Lysates: 40 ug per lane Primary antibody: 1/100dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FBXO32 antibody

Predicted band size: 25 kDa . Positive control: HT-29 cell and human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: HT-29 cell lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/200dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FBXO32 antibody

WB Suggested Anti-FBXO32 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBXO32 antibody

WB Suggested Anti-Fbxo32 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBXO32 Control Peptide

FBXO32
0.25 mg / €250.00
  Acris Antibodies GmbH

FBXO32 (242-264) antibody

Western Blot of FBXO32 antibody (AP09904PU-N at 1/500 dilution). Apparent MW = 43 kDa Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

FBXO32 antibody

Western Blot analysis of FBXO32 expression in transfected 293T cell line (H00114907-T01) by FBXO32 MaxPab polyclonal antibody.

Lane 1: FBXO32 transfected lysate(39.05 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

FBXO32 antibody

FBXO32 Mouse IgG2a 1D6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

FBXO32 (C-term) antibody

Mouse Heart; WB Suggested Anti-Fbxo32 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Fbxo32 antibody - C-terminal region (ARP52866_P050) in Mouse Heart cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

FBXO32 antibody

Western Blot: Fbx32 Antibody [NBP1-36955] - Fbx32 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FBXO32 antibody

FBXO32 Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

FBXO32 293T Cell Transient Overexpression Lysate(Denatured)

FBXO32 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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