FBXO4 antibody

Principal name

FBXO4 antibody

Alternative names for FBXO4 antibody

F-box only protein 4, FBX4

SwissProt ID

Q3T0J1 (Bovin), Q8CHQ0 (Mouse), Q9UKT5 (Human)

Gene ID

26272 (FBXO4), 106052

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of FBXO4 antibody

FBXO4 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO4 belongs to the Fbxs class.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.

General readings

Zeng,Z., et.al., Dev. Cell 18 (2), 214-225 (2010)
Barbash,O., et.al., Cancer Cell 14 (1), 68-78 (2008)
Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO4 (N-term) antibody

Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 was detected using Rockland’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA. Rabbit IgG Serum Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FBXO4 antibody

TA326314 (0.5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBXO4 antibody

WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBXO4 antibody

WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: OVCAR-3 cell lysateFBXO4 is supported by BioGPS gene expression data to be expressed in OVCAR3 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBXO4 (Center) antibody

Western blot analysis of FBXO4 Antibody (Center) Cat.-No AP51631PU-N in Y79 cell line lysates (35ug/lane). FBXO4 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FBXO4 antibody

Western Blot analysis of FBXO4 expression in transfected 293T cell line (H00026272-T01) by FBXO4 MaxPab polyclonal antibody.

Lane 1: FBXO4 transfected lysate(42.57 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

FBXO4 antibody

Western Blot analysis of FBXO4 expression in transfected 293T cell line (H00026272-T01) by FBXO4 MaxPab polyclonal antibody.

Lane 1: FBXO4 transfected lysate(42.57 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

FBXO4 antibody

FBXO4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FBXO4 expression in human pancreas. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

FBXO4 antibody

FBXO4 MaxPab rabbit polyclonal antibody. Western Blot analysis of FBXO4 expression in human pancreas. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FBXO4 antibody

FBXO4 Mouse IgG2a 2F2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

FBXO4 antibody

Immunofluorescence staining of NIH/3T3 cells with FBX4 polyclonal antibody ( Cat # PAB10205 ) . Cells were fixed in methanol/acetone followed by incubation with FBX4 polyclonal antibody at a 1 : 300 dilution.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA. Rabbit IgG Hu, Ms ICC/IF, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

FBXO4 antibody

Human 293T; WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; FBXO4 antibody - middle region (ARP43122_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FBXO4 antibody

Human OVCAR-3; WB Suggested Anti-FBXO4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: OVCAR-3 cell lysate. FBXO4 is supported by BioGPS gene expression data to be expressed in OVCAR3.; FBXO4 antibody - middle region (ARP43123_P050) in Human OVCAR-3 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

FBXO4 antibody

Immunocytochemistry: FBXO4 Antibody [NBP1-78036] - 3T3 cells with Rockland’s anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, Rabbit Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBXO4 (transcript variant 1)

FBXO4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FBXO4

FBXO4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FBXO4

FBXO4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

FBXO4 (Human) IP-WB Antibody Pair

FBXO4 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

FBXO4 293T Cell Transient Overexpression Lysate(Denatured)

FBXO4 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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