FBXO5 antibody

Principal name

FBXO5 antibody

Alternative names for FBXO5 antibody

F-box only protein 5, EMI1, FBX5, Early mitotic inhibitor 1

SwissProt ID

Q7TSG3 (Mouse), Q9UKT4 (Human)

Gene ID

26271 (FBXO5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FBXO5 antibody

FBXO5 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class and is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Lei, S.F., et al. J. Bone Miner. Res. (2010) In press.
Ma, H.T., et al. Mol. Cell. Biol. 29(24):6500-6514(2009).
Lee, J., et al. Mol. Biol. Cell 20(7):1891-1902(2009)
Gutgemann, I., et al. Mod. Pathol. 21(4):445-454(2008)
Iwai, H., et al. Cell 130(4):611-623(2007).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO5 antibody

WB Suggested Anti-FBXO5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FBXO5 antibody

Hum. Adult Placenta; Host:Rabbit. Target Name:FBXO5. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; FBXO5 antibody - C-terminal region (ARP43125_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

FBXO5 antibody

WB using Anti-Fbp5A antibody shows detection of a major band corresponding to Fbp5A protein in a human HeLa whole cell lysate (lane 1 arrowhead). The identity of cross-reactive minor bands at ~72 kDa and 150 kDa is unknown. Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Primary antibody was used at 1:500. Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FBXO5 (Center) antibody

Western blot analysis of FBXO5 Antibody (Center) Cat.-No AP51634PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the FBXO5 antibody detected the FBXO5 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FBXO5 antibody

FBXO5 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FBXO5 antibody

FBXO5 Mouse IgG1 5H7 Purified Hu E
50 µg / €370.00
  Abnova Taiwan Corp.

FBXO5 antibody

FBXO5 Mouse IgG1 5H7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

FBXO5 antibody

Immunohistochemistry of FBXO5 polyclonal antibody ( Cat # PAB10207 ) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. Chimp, Hu
0.1 mg / €560.00
  Abnova Taiwan Corp.

FBXO5 antibody

Western Blot: Fbxo31 Antibody [NBP1-55050] - This Anti-FBXO5 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

FBXO5 antibody

Western Blot: HUS1B Antibody [NBP1-77749] anti-Hus1B antibody shows detection of a 36kDa band corresponding to Hus1B in a HeLa cell lysate (arrowhead). The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Rabbit Aff - Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

FBXO5 antibody

Immunohistochemistry-Paraffin: EMI1 Antibody [NBP1-84850] - Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FBXO5

FBXO5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FBXO5

FBXO5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

FBXO5 overexpression lysate

FBXO5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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