FBXO9 antibody

Principal name

FBXO9 antibody

Alternative names for FBXO9 antibody

F-box only protein 9, NY-REN-57, FBX9, VCIA1, Cross-immune reaction antigen 1

SwissProt ID

Q3ZBT2 (Bovin), Q5U2X1 (Rat), Q8BK06 (Mouse), Q9UK97 (Human)

Gene ID

26268 (FBXO9), 71538 (Fbxo9), 300849

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Frog, Mky (Monkey), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Chimp (Chimpanzee), Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FBXO9 antibody

Kit Component:
- KN305839G1, Fbxo9 gRNA vector 1 in pCas-Guide vector
- KN305839G2, Fbxo9 gRNA vector 2 in pCas-Guide vector
- KN305839D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FBXO9 (431-447) antibody

Western blot using affinity purified anti-FBOX9 antibody shows detection of a band at ~100 kDa (arrowhead) believed to correspond to FBOX9 present in a MCF7 whole cell lysate (lane 1). Specific band reactivity is greatly diminished when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red). IRDye (TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Bov, Can, Chimp, Chk, Hu, Ms, Rt, Xen, Ze E, WB
0.1 mg / €460.00
  OriGene Technologies GmbH

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