FEN1 / RAD2 antibody

Principal name

FEN1 / RAD2 antibody

Alternative names for FEN1 / RAD2 antibody

Flap endonuclease 1, FEN-1, Maturation factor 1, MF1, Flap structure-specific endonuclease 1, DNase IV

SwissProt ID

P39748 (Human), P39749 (Mouse), Q58DH8 (Bovin)

Gene ID

2237 (FEN1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Sh (Sheep), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Hst (Hamster)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Dot blot (Dot)

Background of FEN1 / RAD2 antibody

FEN1 removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

General readings

Hakoyama T., et al. (2009) Nature. 462(7272):514-7.
Lal A., et al. (2009) Mol Cell. 35(5):610-25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FEN1 / RAD2 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762) Mouse IgG1 OTI1F3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using FEN1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

Western blot analysis of anti-FEN1 Antibody (Center) (Cat.#TA324746) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

Western blot analysis of extracts of various cell lines, using FEN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

WB Suggested Anti-FEN1 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate.FEN1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG1 7H8-F4-C11 Purified Hst WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

Western blot detection of FEN-1 in Hela,Jurkat,3T3,C6,CEM and Ramos cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG1 1E7-A6-H6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Mouse IgG2a OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802764) Mouse IgG1 OTI3A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

Immunohistochemistry (IHC) analyzes of FEN-1 antibody (Cat.-No.: AP06687PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FEN1 / RAD2 (N-term) antibody

Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 mcg/lane) by Western blotting with Rabbit anti Human Fen1 (SP2106P). Immunoprecipitation was with SP2106P (3mcg/mg lysate) or another anti Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used SP2106P Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

FEN1 / RAD2 antibody

FEN1 polyclonal antibody (A01), Lot # 051011JC01 Western Blot analysis of FEN1 expression in Hela NE ( Cat # L013V3 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FEN1 / RAD2 antibody

FEN1 / RAD2 Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

FEN1 / RAD2 antibody

FEN1 MaxPab rabbit polyclonal antibody. Western Blot analysis of FEN1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FEN1 / RAD2 antibody

FEN1 monoclonal antibody (M01A), clone 1E2 Western Blot analysis of FEN1 expression in HeLa NE ( Cat # L013V3 ). Mouse IgM 1E2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

FEN1 / RAD2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

FEN1 / RAD2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

FEN1 / RAD2 antibody

Human HepG2; WB Suggested Anti-FEN1 Antibody Titration: 1.25ug/ml. Positive Control: HepG2 cell lysate. FEN1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; FEN1 antibody - C-terminal region (ARP46097_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

FEN1 / RAD2 antibody

Immunohistochemical analysis of FEN1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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