FEN1 / RAD2 antibody

Principal name

FEN1 / RAD2 antibody

Alternative names for FEN1 / RAD2 antibody

Flap endonuclease 1, FEN-1, Maturation factor 1, MF1, Flap structure-specific endonuclease 1, DNase IV

SwissProt ID

P39748 (Human), P39749 (Mouse), Q58DH8 (Bovin)

Gene ID

2237 (FEN1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Hst (Hamster)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of FEN1 / RAD2 antibody

FEN1 removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5\' overhanging flaps in DNA repair and processes the 5\' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5\' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

General readings

Hakoyama T., et al. (2009) Nature. 462(7272):514-7.
Lal A., et al. (2009) Mol Cell. 35(5):610-25.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FEN1 / RAD2 antibody

  FEN1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762) Mouse IgG1 OTI1F3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

  Rabbit polyclonal anti-FEN1 antibody  
Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using FEN1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  Rabbit polyclonal FEN1 Antibody (Center)  
Western blot analysis of anti-FEN1 Antibody (Center) (Cat.#TA324746) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  Rabbit anti-FEN1 Polyclonal Antibody  
Western blot analysis of extracts from 293 cells, treated with serum (20%, 15mins), using PDCD4 (Phospho-Ser457) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  Rabbit Polyclonal Anti-FEN1 Antibody  
WB Suggested Anti-FEN1 Antibody Titration: 1.25 ug/ml; Positive Control: HepG2 cell lysate.FEN1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  Mouse Monoclonal FEN-1 Antibody  
Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG1 7H8-F4-C11 Purified Hst WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  Mouse Monoclonal FEN-1 Antibody  
Western blot detection of FEN-1 in Hela,Jurkat,3T3,C6,CEM and Ramos cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG1 1E7-A6-H6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FEN1 / RAD2 antibody

  FEN1 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Mouse IgG2a OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

  FEN1 mouse monoclonal antibody, clone OTI3A8 (formerly 3A8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA802764) Mouse IgG1 OTI3A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

  FEN1 mouse monoclonal antibody, clone OTI2B2 (formerly 2B2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1. Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FEN1 / RAD2 antibody

Immunohistochemistry (IHC) analyzes of FEN-1 antibody (Cat.-No.: AP06687PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FEN1 / RAD2 (N-term) antibody

Detection of human Fen1 in whole cell lysates of HeLa and 293T cells (5-50 mcg/lane) by Western blotting with Rabbit anti Human Fen1 (SP2106P). Immunoprecipitation was with SP2106P (3mcg/mg lysate) or another anti Fen1 polyclonal antibody, subsequent detection of immunoprecipitated Fen1 by Western blotting used SP2106P Rabbit IgG Aff - Purified Hu, Ms IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

FEN1 / RAD2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

FEN1 / RAD2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FEN1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

FEN1 - human gene knockout kit via CRISPR

FEN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FEN1 - human gene knockout kit via CRISPR

FEN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FEN1 - human gene knockout kit via CRISPR

FEN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FEN1 - human gene knockout kit via CRISPR

FEN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FEN1 / RAD2

  Recombinant protein of human flap structure-specific endonuclease 1 (FEN1)  
FEN1 / RAD2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

FEN1 / RAD2

  Recombinant protein of human flap structure-specific endonuclease 1 (FEN1)  
FEN1 / RAD2 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

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