FERMT2 / PLEKHC1 antibody
Principal name
FERMT2 / PLEKHC1 antibody
Alternative names for FERMT2 / PLEKHC1 antibody
Kindlin 2, Kindlin-2, KIND2, MIG2, MIG-2, Fermitin family homolog 2, Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, PH domain-containing family C member 1
Gene ID
10979 (FERMT2)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F)
Background of FERMT2 / PLEKHC1 antibody
Mig-2 recruits migfilin to cell-matrix adhesions, while the interaction with filamin mediates the association of migfilin with actin filaments. Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation. Mig-2 expression is transcriptionally elevated in leiomyomas and could be involved in its hormone-mediated growth of leiomyomas of the uterus. Expression of Mig-2 is ubiquitous, and it is found in numerous tumor tissues.
Western Blot analysis of PLEKHC1 expression in transfected 293T cell line by PLEKHC1 monoclonal antibody (M09), clone 2G11.Lane 1: PLEKHC1 transfected lysate(77.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical of paraffin-embedded human ovary tumor using 11453-1-AP(FERMT2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using FERMT2 antibody 11453-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP(FERMT2 antibody) at dilution of 1:500
Human placenta tissue were subjected to SDS PAGE followed by western blot with 11453-1-AP(FERMT2 antibody) at dilution of 1:1500
IP Result of anti-FERMT2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.
Western Blot: Kindlin-2 Lysate [NBL1-10677] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for FERMT2
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded colon tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FERMT2 mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)
Western Blot: Kindlin-2 Antibody (9E4) [NBP1-47745] - Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Western Blot: Kindlin-2 Antibody (9E4) [NBP1-47745] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemistry-Paraffin: Kindlin-2 Antibody [NBP1-87884] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunofluorescence: Kindlin-2 Antibody [NBP1-87884] - Immunofluorescent staining of human cell line U-2 OS shows positivity in focal adhesions.
Western Blot: Kindlin-2 Antibody [NBP1-87884] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE GTX118358 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Hepatoma, using Mig-2(GTX118358) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: PC-12 7.5% SDS PAGE GTX118359 diluted at 1:3000
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX118359 diluted at 1:3000
Immunohistochemical analysis of paraffin-embedded Hepatoma, using Mig-2(GTX118359) antibody at 1:500 dilution.
Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (GTX84504).
Anti-FERMT2 mouse monoclonal antibody (GTX84504) immunofluorescent staining of COS7 cells transiently transfected with FERMT2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. (GTX84505, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. (GTX84505, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (GTX84505).
Immunoprecipitation (IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. (GTX84506, Dilution 1:50)
AntiFERMT2 mouse monoclonal antibody (GTX84506) immunofluorescent staining of COS7 cells transiently transfected with FERMT2
Anti-FERMT2 mouse monoclonal antibody (GTX84506) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (GTX84506).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemical staining of paraffin-embedded colon tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-FERMT2 mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (GTX84507).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-FERMT2mouse monoclonal antibody. (GTX84507, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500504).
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500505)
Anti-FERMT2 mouse monoclonal antibody (TA500505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500505).
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500507).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (TA500509).
Anti-FERMT2 mouse monoclonal antibody (TA500509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
Immunoprecipitation of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FERMT2 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
- Quick-Filters:
- Highly Validated (5)
- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500505 | FERMT2 / PLEKHC1 antibody |
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Mouse | IgG2a | OTI14A11 | Purified | Can, Hu, Mky, Rt | ICC/IF, IP, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Top Selling product! Highly validated antibody!
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| TA500504 | FERMT2 / PLEKHC1 antibody |
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Mouse | IgG2a | OTI9E4 | Purified | Can, Hu, Mky, Rt | ICC/IF, IP, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
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| TA500507 | FERMT2 / PLEKHC1 antibody |
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 |
Mouse | IgG2a | OTI25A4 | Purified | Hu, Mky | ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA500509 | FERMT2 / PLEKHC1 antibody |
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 |
Mouse | IgG2a | OTI22E7 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| NBP1-47745 | FERMT2 / PLEKHC1 antibody |
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![Immunohistochemistry-Paraffin: Kindlin-2 Antibody (9E4) [NBP1-47745] - Immunohistochemical staining of paraffin-embedded colon tissue using anti-FERMT2mouse monoclonal antibody. (Dilution 1:50)](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Kindlin-2 Antibody (9E4)-Immunohistochemistry-Paraffin-NBP1-47745-img0004.jpg) |
Mouse | 9E4 | Aff - Purified | Can, Hu, Mky | ICC/IF, IP, P, WB |
0.1 ml /
€620.00
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| Novus Biologicals Inc. | |||||||
| +9 additional images |
Highly validated antibody! |
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| TA332020 | FERMT2 / PLEKHC1 antibody |
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| H00010979-M09 | FERMT2 / PLEKHC1 antibody |
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Mouse | IgG2a | 2G11 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| NBP1-87884 | FERMT2 / PLEKHC1 antibody |
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![Immunohistochemistry-Paraffin: Kindlin-2 Antibody [NBP1-87884] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Kindlin-2 Antibody-Immunohistochemistry-Paraffin-NBP1-87884-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
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| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305727 | FERMT2 / PLEKHC1 (transcript variant 1) |
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
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| OriGene Technologies, Inc. | |||||
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| H00010979-P01-10 | FERMT2 / PLEKHC1 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
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| Abnova Taiwan Corp. | |||||
| H00010979-P01-25 | FERMT2 / PLEKHC1 |
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Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
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| Abnova Taiwan Corp. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY427519 | FERMT2 overexpression lysate |
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0.1 mg /
€480.00
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| OriGene Technologies, Inc. |
