FERMT2 / PLEKHC1 antibody
Principal name
FERMT2 / PLEKHC1 antibody
Alternative names for FERMT2 / PLEKHC1 antibody
Kindlin 2, Kindlin-2, KIND2, MIG2, MIG-2, Fermitin family homolog 2, Mitogen-inducible gene 2 protein, Pleckstrin homology domain-containing family C member 1, PH domain-containing family C member 1
Ncbi ID
9606, NP_001128471.1, NM_001134999, NP_006823, NM_146054, NM_006832, NM_001135000, NP_666166.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of FERMT2 / PLEKHC1 antibody
- Kit Component:
- - KN305898G1, Fermt2 gRNA vector 1 in pCas-Guide vector
- KN305898G2, Fermt2 gRNA vector 2 in pCas-Guide vector
- KN305898D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Western Blot analysis of PLEKHC1 expression in transfected 293T cell line by PLEKHC1 monoclonal antibody (M09), clone 2G11.Lane 1: PLEKHC1 transfected lysate(77.9 KDa).Lane 2: Non-transfected lysate.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500504)
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500504).
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500505)
Anti-FERMT2 mouse monoclonal antibody (TA500505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500505).
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500507)
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500507).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (TA500509).
Anti-FERMT2 mouse monoclonal antibody (TA500509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
Immunoprecipitation of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FERMT2 monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
Host: Rabbit; Target Name: FERMT2; Sample Tissue: HT1080 Whole Cell lysates; Antibody Dilution: 1.0ug/ml. FERMT2 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500505 | FERMT2 / PLEKHC1 antibody |
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| anti-FERMT2 mouse monoclonal antibody, clone OTI14A11 (formerly 14A11) | |||||||
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Mouse | IgG2a | OTI14A11 | Purified | Can, Hu, Mky, Rt | ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Top Selling product! Highly validated antibody!
|
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| TA500504 | FERMT2 / PLEKHC1 antibody |
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| anti-FERMT2 mouse monoclonal antibody, clone OTI9E4 (formerly 9E4) | |||||||
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Mouse | IgG2a | OTI9E4 | Purified | Can, Hu, Mky, Rt | ICC/IF, IP, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +11 additional images |
Highly validated antibody!
|
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| TA500507 | FERMT2 / PLEKHC1 antibody |
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| anti-FERMT2 mouse monoclonal antibody, clone OTI25A4 (formerly 25A4) | |||||||
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Mouse | IgG2a | OTI25A4 | Purified | Hu, Mky | ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA500509 | FERMT2 / PLEKHC1 antibody |
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| anti-FERMT2 mouse monoclonal antibody, clone OTI22E7 (formerly 22E7) | |||||||
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Mouse | IgG2a | OTI22E7 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA332020 | FERMT2 / PLEKHC1 antibody |
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| Rabbit Polyclonal Anti-FERMT2 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN305898LP | Fermt2 - mouse gene knockout kit via CRISPR |
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Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN305898RB | Fermt2 - mouse gene knockout kit via CRISPR |
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Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305727 | FERMT2 / PLEKHC1 (transcript variant 1) |
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| Recombinant protein of human fermitin family homolog 2 (Drosophila) (FERMT2), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
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Lysates
| Catalog No. | ||
|---|---|---|
| LY427519 | FERMT2 overexpression lysate |
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0.1 mg /
€480.00
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| OriGene Technologies, Inc. |
Human Lenti ORF Particles
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209996L2V | Lenti ORF particles, Fermt2 (GFP-tagged) - Mouse fermitin family homolog 2 (Drosophila) (Fermt2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,360.00
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| OriGene Technologies, Inc. | ||
| MR209996L1V | Lenti ORF particles, Fermt2 (Myc-DDK-tagged) - Mouse fermitin family homolog 2 (Drosophila) (Fermt2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,220.00
|
|
| OriGene Technologies, Inc. | ||
