FFAR1 / GPR40 antibody

Principal name

FFAR1 / GPR40 antibody

Alternative names for FFAR1 / GPR40 antibody

Free fatty acid receptor 1, G-protein coupled receptor 40

SwissProt ID

O14842 (Human), Q76JU9 (Mouse), Q8K3T4 (Rat)

Gene ID

2864 (FFAR1), 233081 (Ffar1), 266607

Available reactivities

Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Gorilla, Rb (Rabbit), Rt (Rat), Can (Canine)

Available hosts

Rabbit, Goat, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunofluorescence (IF)

Background of FFAR1 / GPR40 antibody

Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease. Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-termil tail and an intracellular C-terminus. FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids, whereas FFAR2 and FFAR3 are activated by short chain fatty acids. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP. Distribution studies of all three FFA Receptors indicate that FFAR1 mR is quite elevated in the pancreas. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain; although this broad localization pattern is supported only by one study. FFAR2 is expressed in many tissues like liver and colon. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow. As mentioned above, the finding that FFAsâ?? actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control.

General readings

Hardy S, St-Onge GG, Joly E, Langelier Y, Prentki M. Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40. J Biol Chem. 2005 Apr 8;280(14):13285-91. Epub 2005 Feb 4.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FFAR1 / GPR40 (C-term) antibody

TA303026 (0.01µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 (C-term) antibody

Anti-FFAR1 / GPR40 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Gorilla, Hu, Mky, Rb P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 (1st Cytopl. Dom) antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Gorilla, Hst, Hu, Mky, Ms, Por P
50 µg / €345.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

Western blot analysis of rat pancreas lysate: 1. Anti-Free Fatty Acid Receptor 1 (extracellular) antibody, (1:200). 2. Anti-Free Fatty Acid Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Liver Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

Western blot analysis of GPR40 Antibody Cat.-No. AP01314PU-N in extracts from COS-7 cells treated with forskolin 40nM 30'. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FFAR1 / GPR40 antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

FFAR1 / GPR40 (C-term) antibody

FFAR1 Antibody (C-term) (Cat. #AP51649PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FFAR1 / GPR40 antibody

Immunofluorescence analysis of HEK 293T cells overexpressing human FFAR1, using Ffar1 monoclonal antibody, clone G16 ( Cat # MAB1714 ) . Mouse IgG2a G16 Ms F, ICC/IF
25 µg / €750.00
  Abnova Taiwan Corp.

FFAR1 / GPR40 antibody

FFAR1 polyclonal antibody ( Cat # PAB6794 ) ( 0.01 µg/ml ) staining of human breast lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

FFAR1 / GPR40 antibody

Human Liver; WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Liver; FFAR1 antibody - N-terminal region (AVARP08008_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

FFAR1 / GPR40 antibody

FFAR1 / GPR40 Mouse IgG2a G16 Purified Hu IF
25 µg / €760.00
  Funakoshi Co., Ltd.

FFAR1 / GPR40 antibody

Western Blot: GPR40 Antibody [NB100-1537] - NB100-1537 (0.01ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FFAR1 / GPR40 antibody

Western Blot: GPR40 Antibody [NBP1-00860] - Western blot (WB) analysis of GPR40 (W224) antibody in extracts from COS-7 cells treated with forskolin 40nM 30' Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

FFAR1 / GPR40 antibody

Western Blot: GPCR GPR40 Antibody (EP4632) [NBP1-95534] - 3, (C) HeLa, and (D) Neuro-2a cell lysates using anti-GPR40. Rabbit EP4632 Purified Hu, Ms, Rt F, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

FFAR1 overexpression lysate

FFAR1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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