FFAR1 / GPR40 antibody

Principal name

FFAR1 / GPR40 antibody

Alternative names for FFAR1 / GPR40 antibody

Free fatty acid receptor 1, G-protein coupled receptor 40

SwissProt ID

O14842 (Human), Q76JU9 (Mouse), Q8K3T4 (Rat)

Gene ID

2864 (FFAR1), 233081 (Ffar1), 266607

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Mky (Monkey), Rb (Rabbit), Gorilla, Hst (Hamster)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P), Immunofluorescence (IF)

Background of FFAR1 / GPR40 antibody

Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease. Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-termil tail and an intracellular C-terminus. FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids, whereas FFAR2 and FFAR3 are activated by short chain fatty acids. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP. Distribution studies of all three FFA Receptors indicate that FFAR1 mR is quite elevated in the pancreas. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain; although this broad localization pattern is supported only by one study. FFAR2 is expressed in many tissues like liver and colon. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow. As mentioned above, the finding that FFAsâ?? actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control.

General readings

Hardy S, St-Onge GG, Joly E, Langelier Y, Prentki M. Oleate promotes the proliferation of breast cancer cells via the G protein-coupled receptor GPR40. J Biol Chem. 2005 Apr 8;280(14):13285-91.

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FFAR1 / GPR40 (C-term) antibody

  Goat Polyclonal Antibody against GPR40  
TA303026 (0.01µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 (C-term) antibody

  GPR40 Rabbit Polyclonal (C-Terminus) Antibody  
Anti-FFAR1 / GPR40 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Gorilla, Hu, Mky, Rb P, WB
50 µg / €400.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 (1st Cytopl. Dom) antibody

  GPR40 Rabbit Polyclonal (Cytoplasmic Domain) Antibody  
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Gorilla, Hst, Hu, Mky, Ms, Por P
50 µg / €400.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

  Rabbit Polyclonal Anti-Free Fatty Acid Receptor 1 (extracellular)  
Western blot analysis of rat pancreas lysate: 1. Anti-Free Fatty Acid Receptor 1 (extracellular) antibody, (1:200). 2. Anti-Free Fatty Acid Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

  Rabbit Polyclonal Anti-FFAR1 Antibody  
WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human Liver Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FFAR1 / GPR40 antibody

Western blot analysis of GPR40 Antibody Cat.-No. AP01314PU-N in extracts from COS-7 cells treated with forskolin 40nM 30'. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FFAR1 / GPR40 antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

FFAR1 / GPR40 (C-term) antibody

FFAR1 Antibody (C-term) (Cat. #AP51649PU-N) western blot analysis in CEM cell line lysates (35µg/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

FFAR1 - human gene knockout kit via CRISPR

FFAR1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FFAR1 - human gene knockout kit via CRISPR

FFAR1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FFAR1 - human gene knockout kit via CRISPR

FFAR1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FFAR1 - human gene knockout kit via CRISPR

FFAR1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ffar1 - mouse gene knockout kit via CRISPR

Ffar1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ffar1 - mouse gene knockout kit via CRISPR

Ffar1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ffar1 - mouse gene knockout kit via CRISPR

Ffar1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ffar1 - mouse gene knockout kit via CRISPR

Ffar1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

FFAR1 overexpression lysate

FFAR1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ffar1 (GFP-tagged) - Mouse free fatty acid receptor 1 (Ffar1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ffar1 (GFP-tagged) - Mouse free fatty acid receptor 1 (Ffar1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Ffar1 (Myc-DDK-tagged) - Mouse free fatty acid receptor 1 (Ffar1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ffar1 (Myc-DDK-tagged) - Mouse free fatty acid receptor 1 (Ffar1), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

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