FGF acidic / FGF1 antibody

Principal name

FGF acidic / FGF1 antibody

Alternative names for FGF acidic / FGF1 antibody

FGFA, Acidic fibroblast growth factor, ECGF-beta, HBGF-1, HBGF1, Beta-endothelial cell growth factor, Heparin-binding growth factor 1

SwissProt ID

P03968 (Bovin), P05230 (Human), P18651 (Canfa), P19596 (Chick), P20002 (Pig), P61148 (Mouse), P61149 (Rat)

Gene ID

2246 (FGF1), 281160, 25317 (Fgf1), 14164

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Radioimmunoassay (R), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Neutralisation (NEUT)

Background of FGF acidic / FGF1 antibody

Endothelial cell growth factor (ECGF) is an extract of bovine brain containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth factor is prepared using a modification of the method of Maciag, et al. (1979) lyophilized from a sterile solution containing NaCl and streptomycin sulfate.
ECGFpro2 is supplemented with recombinant human VEGF-C (corresponding to 50 ng/ml) a concentration sufficient for the cultivation of lymphatic endothelial cells.
Lymphatic endothelial cells from human skin can be established as primary cultures by traditional methods. The serial propagation of these cells has proved to be difficult. The long-term propagation of these cells in vitro can be achieved with an extract prepared from bovine brain. The introduction of a fibronectin or collagen matrix to the cell culture system allows cultivating endothelial cells at clonal densities. With ECGF, the FCS requirement can be reduced. Heparin potentiates the mitogenic activity of crude preparations of ECGF. ECGF has also been reported to eliminate the need for feeder cells in the clonal growth of hybridomas and other cell types.

General readings

Jaye M. et al., (1986) Science. 233, 541-544.Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129.Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 49-54.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FGF acidic / FGF1 antibody

FGF acidic / FGF1 Mouse IgG1 OTI4D2 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

FGF acidic / FGF1 (46-155) antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2E12 Purified Hu E, ICC/IF, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FGF acidic / FGF1 (N-term) antibody

Western blot analysis of FGF1 (arrow) using rabbit polyclonal  FGF1 Antibody (N-term) (Cat.#TA302125). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGF1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using FGF1 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

Predicted cell location: Cytoplasm . Positive control: Human esophagus and gastric cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using FGF1 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

To detect hFGF-acidic by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-acidic. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

To detect Human FGF-acidic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-acidic (TA328308) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-acidic. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

WB Suggested Anti-Fgf1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Rat Kidney Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

WB Suggested Anti-Fgf1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Mouse Heart Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF acidic / FGF1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1F9 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

Immunohistochemistry of FGF-1 staining in crysections of Bovine oviduct. The sections were treated with 0.5% H2O2 in PBS to reduce endogenous peroxidase activity. (A) The section was incubated with AP02008SU (1/5000). Positive staining appears red, the nuclei are stained blue. Scale bar = 12.6 µm. (C) Control section incubated with Rabbit IgG. Scale bar = 20 µm. Gabler C et al. J Reprod Fertil. 1997 Mar;109(2):213-21. Rabbit Serum Bov C, R, WB
0.1 ml / €530.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

Immunohistochemistry of FGF-1 staining in crysections of Bovine oviduct. The sections were treated with 0.5% H2O2 in PBS to reduce endogenous peroxidase activity. (A) The section was incubated with AP02008SU (1/5000). Positive staining appears red, the nuclei are stained blue. Scale bar = 12.6 µm. (C) Control section incubated with Rabbit IgG. Scale bar = 20 µm. Gabler C et al. J Reprod Fertil. 1997 Mar;109(2):213-21. Rabbit Serum Bov C, R, WB
20 µl / €180.00
  Acris Antibodies GmbH

FGF acidic / FGF1 (C-term) antibody

FGF acidic / FGF1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

FGF acidic / FGF1 Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

FGF acidic / FGF1 Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

FGF acidic / FGF1 antibody

FGF acidic / FGF1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FGF acidic / FGF1 antibody

Immunofluorescence of purified MaxPab antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

FGF acidic / FGF1 antibody

Immunofluorescence of purified MaxPab antibody to FGF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

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