FGF basic / FGF2 antibody

Principal name

FGF basic / FGF2 antibody

Alternative names for FGF basic / FGF2 antibody

FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2

SwissProt ID

P03969 (Bovin), P09038 (Human), P12226 (Xenla), P13109 (Rat), P15655 (Mouse), P20003 (Sheep), P48799 (Rabit), P48800 (Chick), Q60487 (Cavpo)

Gene ID

2247 (FGF2), 14173 (Fgf2), 281161, 54250

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Ms (Mouse), Can (Canine), Chk (Chicken), Rb (Rabbit), Ze (Zebrafish), GP (Guinea Pig)

Available hosts

Mouse, Rabbit, Chicken, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Functional assay (FN), Radioimmunoassay (R), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of FGF basic / FGF2 antibody

Fibroblast growth factors (FGFs) are members of a family of polypeptides that are potent regulators of cell proliferation, differentiation and function. The FGF family consists of seven members with 30-50% sequence identity at the amino acid level and with conservation of the positions of two cysteine residues. The factors are designated FGF-1 through FGF-7; the names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). Comparisons of the primary structure have shown that aFGF and bFGF are closely related mitogens with 55% homology. When isolated from natural sources, bFGF usually has an apparent mass of about 18 kDa, but a variety of larger forms, up to 24 kDa, can exist. Natural bFGF exists in three forms, the 154 amino-acid form and two truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues, respectively. Depending on the organ from which it is purified, either the complete or truncated forms of bFGF may be present. In pituitary and brain the complete form predominates, in kidney and corpus luteum only the truncated form can be detected, and in adrenal glands, both forms coexist. Human and bovine bFGF are nearly identical in sequence, differing at only two positions. The largest (154 aa) form of both human and bovine aFGF share all but 12 amino acids. FGFs play crucial roles in normal development, in the maintenance of tissues, and in wound healing and repair. They have been implicated in a wide range of pathological conditions, including tumorigenesis and metastasis. Both aFGF and bFGF exert their mitogenic influence via saturable high-affinity receptors on a variety of cells of mesodermal and neuroectodermal origin, including endothelial cells, smooth muscle cells, fibroblasts, gliomas, chondrocytes, hepatocytes, epithelial cells, and myoblasts. As expected from the sequence homology between the acidic and basic forms of FGF and from their ability to support the proliferation in vitro of the same spectrum of target cells, both aFGF and bFGF interact with the same cell surface receptor.

General readings

General / background references:Gospodarowicz D et al. Structural characterization and biological functions of fibroblast growth factor. Endocr Rev 8:95-114 (1987). PubMed PMID: 2440668
Esch F et al. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci U S A 82:6507-11 (1985). PubMed PMID: 3863109
Gospodarowicz D Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature 249:123-7 (1974). PubMed PMID: 4364816

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FGF basic / FGF2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500012) Mouse IgG2b OTI3D9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 OTI2H11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

Western blot analysis using recombinant rat FGF-2. Lane 1: 10 ng, Lane 2: 50 ng, Lane 3: 100 ng, Lane 4: 250 ng. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using FGF2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

Predicted band size: 31kDa. Positive control: Mouse lung tissue lysate . Recommended dilution: 1/1000-5000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

To detect hFGF-basic by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-basic. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (TA328310) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-basic. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF. Mouse IgG2b OTI3D10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 AS24 Purified Hu C, E
0.5 mg / €400.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 AS25 Biotin Hu C, E
0.2 mg / €400.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using FGF2 mouse mAb with DAB staining. Mouse IgG 2H5G2C11 Ascites Hu E, P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG1 F-343 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 F-216 Aff - Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG1 F-3 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG1 F-198 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG2a F-74 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG2a F-474 Aff - Purified Hu E, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
1 mg / €910.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
0.2 mg / €450.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
0.1 mg / €340.00
  Acris Antibodies GmbH

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