FGF basic / FGF2 antibody

Principal name

FGF basic / FGF2 antibody

Alternative names for FGF basic / FGF2 antibody

FGFB, Heparin-binding growth factor 2, Fibroblast growth factor 2 (basic), BFGF, HBGF-2, HBGF2

SwissProt ID

P03969 (Bovin), P09038 (Human), P12226 (Xenla), P13109 (Rat), P15655 (Mouse), P20003 (Sheep), P48799 (Rabit), P48800 (Chick), Q60487 (Cavpo)

Gene ID

2247 (FGF2), 281161, 14173 (Fgf2), 54250

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Rb (Rabbit), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit, Chicken, Sheep

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Frozen Sections (C), Radioimmunoassay (R), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Neutralisation (NEUT), Flow Cytometry (F)

Background of FGF basic / FGF2 antibody

The FGF family is composed of at least 22 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HBGF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms was used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor. The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thougth to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF's induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis.

General readings

Nakayama T., et al. (2007) Blood. 109(2):1363-72.A E Eriksson., et al. (1991) PNAS. 88: 3441-3445

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FGF basic / FGF2 antibody

  bFGF (FGF2) mouse monoclonal antibody, clone OTI3D9 (formerly 3D9)  
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BFGF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500012) Mouse IgG2b OTI3D9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

  bFGF (FGF2) mouse monoclonal antibody, clone OTI2H11 (formerly 2H11)  
FGF basic / FGF2 Mouse IgG1 OTI2H11 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

  Rabbit polyclonal anti-FGF-2 antibody  
Western blot analysis using recombinant rat FGF-2. Lane 1: 10 ng, Lane 2: 50 ng, Lane 3: 100 ng, Lane 4: 250 ng. Rabbit Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  Anti-FGF2 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm . Positive control: Human breast cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using FGF2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  Anti-FGF2 Rabbit Polyclonal Antibody  
Predicted band size: 31kDa. Positive control: Mouse lung tissue lysate . Recommended dilution: 1/1000-5000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  Biotinylated Anti-Human FGF-basic Rabbit Polyclonal Antibody  
To detect hFGF-basic by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-basic. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  Anti-Human FGF-basic Rabbit Polyclonal Antibody  
To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (TA328310) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-basic. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  Rabbit Polyclonal Anti-FGF2 Antibody  
WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

FGF basic / FGF2 antibody

  bFGF (FGF2) mouse monoclonal antibody, clone OTI3D10 (formerly 3D10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF. Mouse IgG2b OTI3D10 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 AS24 Purified Hu C, E
0.5 mg / €410.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 AS25 Biotin Hu C, E
0.2 mg / €410.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Immunohistochemical analysis of paraffin-embedded human recturn adenocarcinoma tissue showing cytoplasmic localization using FGF2 mouse mAb with DAB staining. Mouse IgG 2H5G2C11 Ascites Hu E, P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG1 F-343 Aff - Purified Hu E, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG1 F-3 Aff - Purified Hu E, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG2a F-74 Aff - Purified Hu E, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Western blot analysis of Human bFGF using Monoclonal anti-Human bFGF antibody Mouse IgG2a F-474 Aff - Purified Hu E, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
1 mg / €910.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
0.2 mg / €450.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

FGF basic / FGF2 Mouse IgG1 KT1 Aff - Purified Hu E
0.1 mg / €340.00
  Acris Antibodies GmbH

FGF basic / FGF2 antibody

Immunohistochemistry of FGF-2 staining in Paraffin sections of Bovine follicule tissue. Endogenous peroxidase activity was blocked by incubating the section in 1% H2O2 in Methanol  for 30 minutes. The section was incubated with AP02009SU (1/1200) and detected using Biotinylated secondary antibody with ABC Kit. DAB was used as the Chromogen. (A) AP02009SU stains the larger vessels in the theca externa, but not lymphatic vessels (arrow). Scale bar = 100 µm. Berisha B et al (2000) J Endocrinol167(3):371-82. Rabbit Serum Bov C, P, R, WB
0.1 ml / €530.00
  Acris Antibodies GmbH

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