FGFR1 antibody

Principal name

FGFR1 antibody

Alternative names for FGFR1 antibody

BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, BFGFR, bFGF-R-1, FLT-2, N-sam, Proto-oncogene c-Fgr, Basic fibroblast growth factor receptor 1, Fms-like tyrosine kinase 2, Fibroblast growth factor receptor 1

SwissProt ID

A4IFL5 (Bovin), P11362 (Human), P16092 (Mouse), P21804 (Chick), P22182 (Xenla), Q04589 (Rat)

Gene ID

2260 (FGFR1), 8622608 (abcC1), 2263 (FGFR2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chimp (Chimpanzee), Bov (Bovine), Mky (Monkey), Chk (Chicken), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F), Dot blot (Dot), Neutralisation (NEUT)

Background of FGFR1 antibody

FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38 kDa) that are potent physiological regulators of growth and differentiation of a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They also have been implicated in pathological conditions such as tumorigenesis and metastasis. To date, the FGF family consists of at least 23 members designated FGF1 through FGF23. Four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg-1(fms-like gene 1)]; FGFR2 [bek (bacterial expressed kinase gene product)]; FGFR3 (cek-2), and FGFR4. Multiple additional variants (isoforms) arising by alternative splicing have been reported: soluble, secreted, or possibly cleaved forms of FGFR1 and FGFR2 have also been found in body fluids or were artificially constructed, [e.g. a soluble FGF-binding protein containing the extracellular region of FGFR1 and the secreted form of placental alkaline phosphatase (FRAP1)].
FGFRs are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110- 150 kDa proteins that are constructed of an extracellular ligand binding region with either two (alpha type) or typically three (alpha type) immunoglbulin (Ig)-like domains and an eight amino acid acidic box, a transmembrane region, and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of FGFRs is confined to the extracellular Ig-like domains 2 and 3.
FGFRs exhibit overlapping recognition and redundant specificity. One receptor type may bind with a similar affinity several of the FGFs. Also one FGF type may bind similarly to several distinct receptors. This accounts for the rather identical effects of different FGF ligands on common cell types. FGF’s binding to cellular FGFRs depend on or is markedly facilitated by the low-affinity interaction of FGF with the polysaccharide component of the cell surface or extracellular matrix heparan sulfate proteoglycans (HSPG). For example, perlecan, a basement membrane HSPG, promotes high affinity binding of FGF2 in vitro and angiogenesis in vivo. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ and are dictated by the intracellular domain.
At the mRNA level, FGFR1 is highly expressed in developing human tissues including the brain (preferentially in neurons), vascular basement membranes, skin, and bone growth plates. It may be found in most anchorage dependent cells on their membrane and also may be localized around and in nuclei. Pfeiffer syndrome, as well as other disorders of human skeletal development, is the result of a mutation in the extracellular domain of FGFR1.

General readings

This product has been used in:Morrison RS et al. Fibroblast growth factor receptor gene expression and immunoreactivity are elevated in human glioblastoma multiforme. Cancer Res 54:2794-9 (1994). PubMed PMID: 8168112
Zhao XM et al. Modification of alternative messenger RNA splicing of fibroblast growth factor receptors in human cardiac allografts during rejection. J Clin Invest 94:992-1003 (1994). PubMed PMID: 7521891
General / background references:Feng S et al. Nuclear localization of a complex of fibroblast growth factor(FGF)-1 and an NH2-terminal fragment of FGF receptor isoforms R4 and R1alpha in human liver cells. Biochim Biophys Acta 1310:67-73 (1996). PubMed PMID: 9244177
Xu J et al. Expression and immunochemical analysis of rat and human fibroblast growth factor receptor (flg) isoforms. J Biol Chem 267:17792-803 (1992). PubMed PMID: 1325449

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FGFR1 (360-373) antibody

AP15549PU FGFR1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

FGFR1 (360-373) antibody

AP15549PU FGFR1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

FGFR1 (360-373) antibody

AP15549PU FGFR1 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

FGFR1 antibody

Western blot analysis of FGFR1 (arrow) using rabbit polyclonal FGFR1 Antibody (Y766) (RB11274). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of FGFR1 (arrow) using rabbit polyclonal FGFR1 Antibody (Y653) (RB11303). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of FGFR1 (arrow) using rabbit polyclonal FGFR1 Antibody (Y463) (RB11175). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 (C-term) antibody

TA302994 (0.3µg/ml) staining of human breast lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Chimp, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

TA309547 (1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of extracts from HepG2 cells, using FGFR1 (Ab-766) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGFR1 (beta isoform, N-term) antibody

Anti-FGFR1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG2b M17A3 Purified Hu IP, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

FGFR1 antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical and liver cancer tissue. Recommended dilution: 1/50-300  The image on the left is immunohistochemistry of paraffin-embedded Human cervical and liver cancer tissue using FGFR1 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of extracts from JurKat cells, using FGFR1/2 (Ab-463) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of extracts of Jurkat cell lines, using FGFR1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

Western blot analysis of FGFR1 expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FGFR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR1 (RC202080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR1. Mouse IgG1 OTI3C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGFR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR1 (RC202080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR1. Mouse IgG2b OTI6F8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGFR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR1 (RC202080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR1. Mouse IgG2b OTI1A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGFR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR1 (RC202080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR1. Mouse IgG1 OTI5D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FGFR1 antibody

Western blot analysis using FGFR1 antibody Cat.-No AM06350SU-N against extracellular domain of human FGFR1 (aa22-376). Mouse IgG2b 3D4F7 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

FGFR1 antibody

Western blot (WB) analysis of FGFR1antibody (Cat.-No.: AP06428PU-N) in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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