FHL1 antibody

Principal name

FHL1 antibody

Alternative names for FHL1 antibody

SLIM1, FHL1, FHL-1, SLIM-1, SLIM, Four and a half LIM domains protein 1, Skeletal Muscle LIM-protein 1

SwissProt ID

P97447 (Mouse), Q13642 (Human), Q9WUH4 (Rat)

Gene ID

2273 (FHL1), 14199 (Fhl1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), African clawed frog, Bov (Bovine), Eq (Equine), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FHL1 antibody

Kit Component:
- KN305960G1, Fhl1 gRNA vector 1 in pCas-Guide vector
- KN305960G2, Fhl1 gRNA vector 2 in pCas-Guide vector
- KN305960D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA. Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication. J Biol Chem. 1999 Sep 17;274(38):27083-91.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FHL1 antibody

  FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)  
Anti-FHL1 mouse monoclonal antibody (TA501279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478). Mouse IgG1 OTI2E11 Purified Can, Hu, Rt F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FHL1 antibody

  anti-FHL1 mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody. Mouse IgG1 OTI1D6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FHL1 antibody

  FHL1 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)  
Anti-FHL1 mouse monoclonal antibody (TA501296) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478). Mouse IgG2b OTI3G7 Purified Hu, Rt F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

FHL1 antibody

  anti-FHL1 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)  
Anti-FHL1 mouse monoclonal antibody (TA501375) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478). Mouse IgG1 OTI3H3 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FHL1 antibody

  Goat Polyclonal Antibody against FHL1 / SLIM1  
TA302486 staining (0.05µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL1 antibody

  Goat Polyclonal Antibody against SLIMMER / FHL1 isoform B  
TA302612 (0.01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL1 antibody

  Rabbit Polyclonal anti-FHL1 antibody  
WB Suggested Anti-FHL1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

FHL1 antibody

  Rabbit Polyclonal Anti-FHL1 Antibody  
WB Suggested Anti-FHL1 Antibody Titration: 0.1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL1 antibody

  Rabbit Polyclonal Anti-FHL1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human adrenal pheochromocytoma tissue, Human fetal muscle tissue, Human skeletal muscle tissue, Primary antibody: (FHL1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FHL1 antibody

  anti-FHL1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)  
Anti-FHL1 mouse monoclonal antibody (TA501280) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478). Mouse IgG1 OTI2E1 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

FHL1 antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

FHL1 (C-term) antibody

Western blot analysis of FHL1 Antibody (C-term) (AP17369PU-N) in NIH-3T3 cell line lysates (35 µg/lane). FHL1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

FHL1 - human gene knockout kit via CRISPR

FHL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FHL1 - human gene knockout kit via CRISPR

FHL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FHL1 - human gene knockout kit via CRISPR

FHL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FHL1 - human gene knockout kit via CRISPR

FHL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fhl1 - mouse gene knockout kit via CRISPR

Fhl1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Fhl1 - mouse gene knockout kit via CRISPR

Fhl1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fhl1 - mouse gene knockout kit via CRISPR

Fhl1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fhl1 - mouse gene knockout kit via CRISPR

Fhl1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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