FHL2 / SLIM3 antibody

Principal name

FHL2 / SLIM3 antibody

Alternative names for FHL2 / SLIM3 antibody

FHL-2, SLIM 3, DRAL, Four and a half LIM domains protein 2, Skeletal muscle LIM-protein 3, LIM domain protein DRAL

SwissProt ID

O35115 (Rat), O70433 (Mouse), Q14192 (Human), Q2KI95 (Bovin)

Gene ID

2274 (FHL2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C)

Background of FHL2 / SLIM3 antibody

The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.

General readings

1: Nair SS, Guo Z, Mueller JM, Koochekpour S, Qiu Y, Tekmal RR, Schule R, Kung HJ, Kumar R, Vadlamudi RK. PELP1/MNAR enhances androgen receptor functions through LIM-only coactivator FHL2. Mol Endocrinol. 2006 Dec 27.
2:Kudo LC, Karsten SL, Chen J, Levitt P, Geschwind DH. Genetic Analysis of Anterior-Posterior Expression Gradients in the Developing Mammalian Forebrain. Cereb Cortex. 2006 Dec 5.
3: Kahl P, Gullotti L, Heukamp LC, Wolf S, Friedrichs N, Vorreuther R, Solleder G, Bastian PJ, Ellinger J, Metzger E, Schule R, Buettner R. Androgen receptor coactivators lysine-specific histone demethylase 1 and four and a half LIM domain protein 2 predict risk of prostate cancer recurrence.Cancer Res. 2006 Dec 1;66(23):11341-7.
4: Campo-Fernandez B, Morandell D, Santer FR, Zwerschke W, Jansen-Durr P. Identification of the FHL2 Transcriptional Coactivator as a New Functional Target of the E7 Oncoprotein of Human Papillomavirus Type 16.J Virol. 2007 Jan;81(2):1027-32. Epub 2006 Nov 8.
5: Govoni KE, Baylink DJ, Chen J, Mohan S. Disruption of four-and-a-half LIM 2 decreases bone mineral content and bone mineral density in femur and tibia bones of female mice. Calcif Tissue Int. 2006 Aug;79(2):112-7. Epub 2006 Aug 15.
6: Sun J, Yan G, Ren A, You B, Liao JK. FHL2/SLIM3 decreases cardiomyocyte survival by inhibitory interaction with sphingosine kinase-1. Circ Res. 2006 Sep 1;99(5):468-76. Epub 2006 Aug 3.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FHL2 / SLIM3 antibody

Immunohistochemistry: FHL2 Antibody [NBP1-33603] - Immunohistochemical analysis of FHL2 on paraffin-embedded SAS xenograft, using NBP1-33603. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

FHL2 / SLIM3 (C-term) antibody

TA305700 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL2 / SLIM3 antibody

WB Suggested Anti-FHL2 Antibody Titration: 0.6 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL2 / SLIM3 antibody

Figure 3. Immunohistochemical detection of FHL2 on Paraffin Embedded section of a Human prostate cancer with FHL2 antibody (AM20016AF-N). Mouse IgG2a 11-134 Azide Free Hu, Ms IP, P, WB
0.1 mg / €410.00
  Acris Antibodies GmbH

FHL2 / SLIM3 antibody

FHL2 / SLIM3 Mouse IgG1 F4B2-B11 Purified Hu, Ms, Rt C, ICC/IF, P, WB
0.1 mg / €520.00
  Acris Antibodies GmbH

FHL2 / SLIM3 control peptide

FHL2 / SLIM3 Purified
50 µg / €210.00
  Acris Antibodies GmbH

FHL2 / SLIM3 antibody

Western blot using AP05258PU-N, rabbit polyclonal at 0.5 µg/ml on HeLa cell extract (10 µg/lane). Blots were developed with goat anti-rabbit Ig (1:75k) and Pierce's Supersignal West Femto system. Rabbit IgG Aff - Purified Hu WB
50 µg / €480.00
  Acris Antibodies GmbH

FHL2 / SLIM3 antibody

Legend: Western blot using AP05258SU-N, rabbit polyclonal at 0.4 µg/ml on HeLa cell extract (10 µg/lane). Blots were developed with goat anti rabbit Ig (1:75k) and Pierce's Supersignal West Femto system. Lane: 1- AP05258SU-N at 0.4 µg/ml. 2- AP05258SU-N plus 16 µg blocking peptide. 3- Goat anti Rabbit HRP control. Rabbit IgG Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

FHL2 / SLIM3 antibody

FHL2 / SLIM3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

Western Blot analysis of FHL2 expression in transfected 293T cell line (H00002274-T01) by FHL2 MaxPab polyclonal antibody.

Lane 1: FHL2 transfected lysate(30.69 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

Western Blot analysis of FHL2 expression in transfected 293T cell line (H00002274-T01) by FHL2 MaxPab polyclonal antibody.

Lane 1: FHL2 transfected lysate(32.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

Immunofluorescence of monoclonal antibody to FHL2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2G3-1A5 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

FHL2 / SLIM3 Goat Hu, Ms E
0.1 mg / €400.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

Western blot using FHL2 polyclonal antibody on HeLa cell extract ( 10 µg/lane ). Lane 1: 0.4 µg/ml, lane 2: plus 16 µg blocking peptide. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

FHL2 / SLIM3 antibody

Human Heart; FHL2 antibody - C-terminal region (ARP34506_T100) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

FHL2 / SLIM3 antibody

Western blot analysis of FHL2 expression in HeLa (A); HepG2 (B); mouse brain (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FHL2 / SLIM3 antibody

Western blot analysis of FHL2 expression in HeLa (A); HepG2 (B); mouse brain (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FHL2 / SLIM3 antibody

Western blot analysis of FHL2 expression in HeLa (A); HepG2 (B); mouse brain (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

FHL2 / SLIM3 antibody

Western Blot: FHL2 Antibody [NB300-804] - 1ug/ml staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FHL2 / SLIM3 antibody

Immunofluorescence: FHL2 Antibody [NBP1-31262] - Immunofluorescence analysis of HeLa, using FHL2 antibody at 1:200 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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