FHL3 antibody

Principal name

FHL3 antibody

Alternative names for FHL3 antibody

FHL-3, SLIM2, Four and a half LIM domains protein 3, Skeletal muscle LIM-protein 2

SwissProt ID

Q13643 (Human), Q3ZBI6 (Bovin), Q9R059 (Mouse)

Gene ID

2275 (FHL3)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine), Chk (Chicken), Dros (Drosophila), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FHL3 antibody

FHL3 is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene.

General readings

1. Samson T, Smyth N, Janetzky S, Wendler O, Muller JM, Schule R, von der Mark H, von der Mark K, Wixler V.
The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor. J Biol Chem. 2004 Jul 2;279(27):28641-52. Epub 2004 Apr 26.
PMID: 15117962

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FHL3 (C-term) antibody

TA305761 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL3 antibody

WB Suggested Anti-FHL3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

FHL3 antibody

FHL3 Mouse Hu ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

FHL3 antibody

Detection limit for recombinant GST tagged FHL3 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 2C10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FHL3 antibody

Human HepG2; WB Suggested Anti-FHL3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; FHL3 antibody - middle region (ARP50498_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

FHL3 antibody

Western Blot: TRC8 Antibody [NBP1-26395]-(1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Goat IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

FHL3 antibody

FHL3 Rabbit IgG Hu WB
0.1 ml / €340.00
  Novus Biologicals Inc.

FHL3 antibody

Immunohistochemistry-Paraffin: FHL3 Antibody [NBP1-91899] Immunohistochemical staining of human skeletal muscle shows moderate nuclear and cytoplasmic positivity in myocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FHL3

FHL3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FHL3 (1-280, His-tag)

FHL3 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

FHL3 (1-280, His-tag)

FHL3 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

FHL3

FHL3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

FHL3 293T Cell Transient Overexpression Lysate(Denatured)

FHL3 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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