FHL3 antibody

Principal name

FHL3 antibody

Alternative names for FHL3 antibody

FHL-3, SLIM2, Four and a half LIM domains protein 3, Skeletal muscle LIM-protein 2

SwissProt ID

Q13643 (Human), Q3ZBI6 (Bovin), Q9R059 (Mouse)

Gene ID

2275 (FHL3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of FHL3 antibody

Kit Component:
- KN203991G1, FHL3 gRNA vector 1 in pCas-Guide vector
- KN203991G2, FHL3 gRNA vector 2 in pCas-Guide vector
- KN203991D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Samson T, Smyth N, Janetzky S, Wendler O, Muller JM, Schule R, von der Mark H, von der Mark K, Wixler V.
The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor. J Biol Chem. 2004 Jul 2;279(27):28641-52. Epub 2004 Apr 26.
PMID: 15117962

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FHL3 (C-term) antibody

  Goat Anti-FHL3 / SLIM2 Antibody  
TA305761 (1ug/ml) staining of Rat Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FHL3 antibody

  Rabbit Polyclonal Anti-FHL3 Antibody  
WB Suggested Anti-FHL3 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

FHL3 antibody

  Rabbit Polyclonal Anti-FHL3 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Mouse skeletal muscle tissue, Primary antibody: (FHL3 Antibody) at dilution 1/1600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FHL3 antibody

FHL3 Mouse Hu ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

FHL3 antibody

Detection limit for recombinant GST tagged FHL3 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 2C10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

FHL3 antibody

Human HepG2; WB Suggested Anti-FHL3 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; FHL3 antibody - middle region (ARP50498_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FHL3 antibody

Western blot analysis of FHL3 expression in K562 (A); HeLa (B); Raw264.7 (C); PC12 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

FHL3 - human gene knockout kit via CRISPR

FHL3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FHL3 - human gene knockout kit via CRISPR

FHL3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FHL3 - human gene knockout kit via CRISPR

FHL3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FHL3 - human gene knockout kit via CRISPR

FHL3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FHL3

  Recombinant protein of human four and a half LIM domains 3 (FHL3)  
FHL3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

FHL3 (1-280, His-tag)

FHL3 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

FHL3 (1-280, His-tag)

FHL3 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

FHL3

FHL3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

FHL3

FHL3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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