Fibulin-1 antibody

Principal name

Fibulin-1 antibody

Alternative names for Fibulin-1 antibody

FIBL-1, FBLN1, PP213

SwissProt ID

O73775 (Chick), P23142 (Human), Q08879 (Mouse)

Gene ID

2192 (FBLN1)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Fibulin-1 antibody

Fibulin-1 is an extracellular matrix protein often associated with fibronectin (FN) in vivo. Fibulin-1 was found to have pronounced inhibitory effects on the cell attachment and spreading promoted by FN. Fibulin-1 was also found to inhibit the motility of a variety of cell types on FN substrata. For example, the FN-dependent haptotactic motility of breast carcinoma (MDA MB231) cells, epidermal carcinoma (A431), melanoma (A375 SM), rat pulmonary aortic smooth muscle cells (PAC1) and Chinese hamster ovary (CHO) cells was inhibited by the presence of fibulin-1 bound to FN-coated Boyden chamber membranes. Cells transfected to overproduce fibulin-1 displayed reduced velocity, distance of movement and persistence time on FN substrata. Similarly, the incorporation of fibulin-1 into FN-containing type I collagen gels inhibited the invasion of endocardial cushion mesenchymal cells migrating from cultured embryonic heart explants. By contrast, incorporation of fibulin-1 into collagen gels lacking FN had no effect on the migration of endocardial cushion cells.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Li, C., et al. Mol. Vis. 16, 689-697 (2010)
Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010)
Argraves, W.S., et al. Histochem. Cell Biol. 132(5):559-565(2009)
Piscaglia, F., et al. Cell Tissue Res. 337(3):449-462(2009).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fibulin-1 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and liver cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus and liver cancer tissue using FBLN1 antibody at dilution 1/47, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fibulin-1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer and brain tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer and brain tissue using FBLN1 antibody at dilution 1/12, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fibulin-1 (C-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Fibulin-1 Antibody (C-term) Cat.-No AP51615PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Fibulin-1 antibody

FBLN1 MaxPab polyclonal antibody. Western Blot analysis of FBLN1 expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

FBLN1 MaxPab polyclonal antibody. Western Blot analysis of FBLN1 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

Fibulin-1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

Western Blot analysis of FBLN1 expression in transfected 293T cell line (H00002192-T01) by FBLN1 MaxPab polyclonal antibody.

Lane 1: FBLN1 transfected lysate(74.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

FBLN1 monoclonal antibody (M01), clone 4C9 Western Blot analysis of FBLN1 expression in A-431 ( Cat # L015V1 ). Mouse IgG2a 4C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

Fibulin-1 Mouse IgG1 2G9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

Fibulin-1 Mouse IgG2a 3E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fibulin-1 antibody

Human OVCAR-3; WB Suggested Anti-FBLN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: OVCAR-3 cell lysate; FBLN1 antibody - N-terminal region (ARP42000_P050) in Human OVCAR-3 cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Fibulin-1 antibody

Western blot analysis of Fibulin 1 expression in A431 (A); A549 (B); HeLa (C); NIH3T3 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Fibulin-1 antibody

Western blot analysis of Fibulin 1 expression in A431 (A); A549 (B); HeLa (C); NIH3T3 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Fibulin-1 antibody

Western blot analysis of Fibulin 1 expression in A431 (A); A549 (B); HeLa (C); NIH3T3 (D); H9C2 (E) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Fibulin-1 antibody

Western Blot: fibulin 1 Antibody [NBP1-57911] - This Anti-FBLN1 antibody was used in Western Blot of OVCAR-3     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Fibulin-1 antibody

Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] Immunohistochemical staining of human placenta shows cytoplasmic and membranous positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Fibulin-1 (transcript variant D)

Fibulin-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Fibulin-1

Fibulin-1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Fibulin-1

Fibulin-1 Human
10 µg / €380.00
  Abnova Taiwan Corp.

Fibulin-1

Fibulin-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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