Fibulin-3 antibody

Principal name

Fibulin-3 antibody

Alternative names for Fibulin-3 antibody

DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, Fibulin 3, EFEMP1, FBLN3, EGF-containing fibulin-like extracellular matrix protein 1, Fibrillin-like protein, Extracellular protein S1-5

SwissProt ID

O35568 (Rat), Q12805 (Human), Q8BPB5 (Mouse)

Gene ID

2202 (EFEMP1), 216616 (Efemp1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Macaque, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of Fibulin-3 antibody

Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target. At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.

General readings

Okada,Y. et.al., Hum. Mol. Genet. 19(11): 2303-2312 (2010)
Wakabayashi,T. et.al., Biochem. Biophys. Res. Commun. 391(1): 1116-1121 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fibulin-3 antibody

  Rabbit Polyclonal Fibulin 3 Antibody  
Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

  Rabbit polyclonal anti-EFEMP1 antibody  
Western blot analysis of extracts from rat lung cells, using EFEMP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

  Rabbit Polyclonal Anti-EFEMP1 Antibody  
WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysateEFEMP1 is supported by BioGPS gene expression data to be expressed in HT1080 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

  Rabbit Polyclonal Anti-EFEMP1 Antibody  
WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

  Rabbit Polyclonal Anti-EFEMP1 Antibody  
WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

  EFEMP1 mouse monoclonal antibody, clone OTI1D9 (formerly 1D9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFEMP1 (RC214953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFEMP1. Mouse IgG2a OTI1D9 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Fibulin-3 antibody

Western blot (WB) analysis of Fibulin-3 antibody (Cat.-No.: AP21171PU-N) in extracts from rat lung cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Fibulin-3 (C-term) antibody

Human Small Intestine, Capillary: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Fibulin-3 (N-term) antibody

Immunohistochemistry analysis in human lung carcinoma (Formalin-fixed, Paraffin-embedded) using Fibulin-3 antibody Cat.-No. AP51374PU-N (N-term), followed by peroxidase-conjugated  secondary antibodyand DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Fibulin-3 antibody

Western blot analysis of extracts from rat lung, using EFEMP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Fibulin-3 antibody

Western blot analysis of extracts from rat lung, using EFEMP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

EFEMP1 - human gene knockout kit via CRISPR

EFEMP1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EFEMP1 - human gene knockout kit via CRISPR

EFEMP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EFEMP1 - human gene knockout kit via CRISPR

EFEMP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EFEMP1 - human gene knockout kit via CRISPR

EFEMP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Efemp1 - mouse gene knockout kit via CRISPR

Efemp1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Efemp1 - mouse gene knockout kit via CRISPR

Efemp1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Efemp1 - mouse gene knockout kit via CRISPR

Efemp1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Efemp1 - mouse gene knockout kit via CRISPR

Efemp1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Fibulin-3 (transcript variant 3)

  Recombinant protein of human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1), transcript variant 3  
Fibulin-3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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