Fibulin-3 antibody

Principal name

Fibulin-3 antibody

Alternative names for Fibulin-3 antibody

DHRD, DRAD, FBNL, MLVT, MTLV, S1-5, Fibulin 3, EFEMP1, FBLN3, EGF-containing fibulin-like extracellular matrix protein 1, Fibrillin-like protein, Extracellular protein S1-5

SwissProt ID

O35568 (Rat), Q12805 (Human), Q8BPB5 (Mouse)

Gene ID

2202 (EFEMP1), 3522020 (BAA-681)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), Mky (Monkey), Macaque, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Fibulin-3 antibody

Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target. At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.

General readings

General / background references:Stone EM et al. A single EFEMP1 mutation associated with both Malattia Leventinese and Doyne honeycomb retinal dystrophy. Nat Genet 22:199-202 (1999). PubMed PMID: 10369267
Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). PubMed PMID: 1832709

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fibulin-3 antibody

Western blot analysis of Fibulin 3 in HeLa cell lysate with Fibulin 3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

Western blot analysis of extracts from rat lung cells, using EFEMP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HT1080 cell lysateEFEMP1 is supported by BioGPS gene expression data to be expressed in HT1080 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Fibulin-3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFEMP1 (RC214953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFEMP1. Mouse IgG2a OTI1D9 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Fibulin-3 Control Peptide

Fibulin-3
0.25 mg / €250.00
  Acris Antibodies GmbH

Fibulin-3 antibody

Fibulin-3 Rabbit Aff - Purified Hu, Mky, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Fibulin-3 antibody

Western blot (WB) analysis of Fibulin-3 antibody (Cat.-No.: AP21171PU-N) in extracts from rat lung cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Fibulin-3 (C-term) antibody

Human Small Intestine, Capillary: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Fibulin-3 Control Peptide

Fibulin-3
50 µg / €160.00
  Acris Antibodies GmbH

Fibulin-3 (N-term) antibody

Immunohistochemistry analysis in human lung carcinoma (Formalin-fixed, Paraffin-embedded) using Fibulin-3 antibody Cat.-No. AP51374PU-N (N-term), followed by peroxidase-conjugated  secondary antibodyand DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Fibulin-3 antibody

Western Blot analysis of EFEMP1 expression in transfected 293T cell line (H00002202-T02) by EFEMP1 MaxPab polyclonal antibody.

Lane 1: EFEMP1 transfected lysate(54.23 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

Fibulin-3 antibody

Western blot analysis of extracts from rat lung, using EFEMP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Fibulin-3 antibody

Western blot analysis of extracts from rat lung, using EFEMP1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Fibulin-3 antibody

Human HT1080; WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HT1080 cell lysate. EFEMP1 is supported by BioGPS gene expression data to be expressed in HT1080.; EFEMP1 antibody - middle region (ARP41341_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Fibulin-3 antibody

Human 721_B; WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate; EFEMP1 antibody - middle region (ARP41342_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Fibulin-3 antibody

Human Placenta; WB Suggested Anti-EFEMP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; EFEMP1 antibody - middle region (ARP41450_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Fibulin-3 antibody

Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human
RPE cells. Antibody EFP-
100P (1:500) Rabbit Aff - Purified Hu, Rt IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Fibulin-3 antibody

Fibulin-3 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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