FITC antibody

Principal name

FITC antibody

Alternative names for FITC antibody

Fluorescein Isothiocyanate

Available reactivities

All Species, Gt (Goat)

Available hosts

Rabbit, Mouse, Goat, Sheep, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunodiffusion (ID)

Background of FITC antibody

Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.

General readings

General / background references:Titus JA et al. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods 50:193-204 (1982). PubMed PMID: 6806389

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FITC antibody

FITC Goat IgG HRP C, Dot, E, WB
1 mg / €580.00
  OriGene Technologies GmbH

FITC antibody

FITC Goat Serum Dot, E, IP, WB
2 ml / €460.00
  OriGene Technologies GmbH

FITC F(ab)2 Fragment antibody

FITC Goat IgG AP C, Dot, E, WB
1 mg / €550.00
  OriGene Technologies GmbH

FITC F(ab)2 Fragment antibody

FITC Goat IgG HRP C, Dot, E, WB
1 mg / €550.00
  OriGene Technologies GmbH

4 Item(s)

per page
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